################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I BalI BbeI BbrPI BciVI BclI BfrI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoT22I FbaI FseI HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcyI 126 GACGCC G[GA]^CG[CT]C AflIII 2369 ACATGT A^C[GA][CT]GT Alw44I 537 GTGCAC G^TGCAC ApaLI 537 GTGCAC G^TGCAC AviII 77 TGCGCA TGC^GCA AvrII 1737 CCTAGG C^CTAGG BamHI 1890 GGATCC G^GATCC BanI 1400 GGTGCC G^G[CT][GA]CC BcgI 232 CGACTGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 752 AGATCT A^GATCT BlnI 1737 CCTAGG C^CTAGG BlpI 1940 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1940 GCTCAGC GC^T[ACGT]AGC BsaHI 126 GACGCC G[GA]^CG[CT]C Bsp1407I 2111 TGTACA T^GTACA BspHI 1794 TCATGA T^CATGA BspLU11I 2369 ACATGT A^CATGT BsrGI 2111 TGTACA T^GTACA BstEII 188 GGTCACC G^GT[ACGT]ACC BstPI 188 GGTCACC G^GT[ACGT]ACC BstXI 1704 CCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1940 GCTCAGC GC^T[ACGT]AGC DraIII 1928 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 188 GGTCACC G^GT[ACGT]ACC EcoRV 1519 GATATC GAT^ATC FspI 77 TGCGCA TGC^GCA HgaI 126 GACGC GACGC(5/10) Hin1I 126 GACGCC G[GA]^CG[CT]C NcoI 650 CCATGG C^CATGG RcaI 1794 TCATGA T^CATGA SacI 1252 GAGCTC GAGCT^C SalI 871 GTCGAC G^TCGAC SspBI 2111 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AhdI 874 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1834 GACCTGCTGTC AspEI 874 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1834 GACCTGCTGTC AspI 1530 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1613 GACGGAGTC BsaI 16 GAGACC GGTCTC(1/5) 464 GAGACC BsrDI 1095 CATTGC GCAATG(2/0) 848 GCAATG EaeI 612 TGGCCG [CT]^GGCC[GA] 2347 CGGCCA Eam1105I 874 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1834 GACCTGCTGTC HaeII 425 GGCGCT [GA]GCGC^[CT] 1651 AGCGCC HincII 640 GTTGAC GT[CT]^[GA]AC 871 GTCGAC HindII 640 GTTGAC GT[CT]^[GA]AC 871 GTCGAC HindIII 1385 AAGCTT A^AGCTT 1462 AAGCTT MaeII 935 ACGT A^CGT 2142 ACGT MslI 561 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 795 CACCAAGATG PflFI 1530 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1613 GACGGAGTC PflMI 950 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2266 CCATCAGATGG StuI 1407 AGGCCT AGG^CCT 1763 AGGCCT TaqI 216 TCGA T^CGA 872 TCGA Tsp509I 2491 AATT ^AATT 2522 AATT Tth111I 1530 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1613 GACGGAGTC TthHB8I 216 TCGA T^CGA 872 TCGA Van91I 950 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2266 CCATCAGATGG XcmI 74 CCATGCGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 243 CCAGAGTGGCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI XhoII XmaCI XmaI