(Circular) (Six-base) MAPSORT of: pFN21AE2118.seq Check: 4319 from: 1 to: 7282 pFN21AE2118 7282 bp With 182 enzymes: SgfI * March 28, 2012 12:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2697 2697 Size: 7282 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5092 278 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2060 2877 4182 4625 1937 Size: 123 817 1305 443 4594 Fragments arranged by size: 4594 1305 817 443 123 Acc65I G'GTAC_C Cuts at: 4603 4603 Size: 7282 AclI AA'CG_TT Cuts at: 1563 5409 5782 1563 Size: 3846 373 3063 Fragments arranged by size: 3846 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 3440 4240 4344 4401 5345 6729 6997 Size: 896 912 800 104 57 944 1384 268 Cuts at: 6997 1632 Size: 1917 Fragments arranged by size: 1917 1384 944 912 896 800 268 104 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7085 AflIII A'CryG_T Cuts at: 1203 4374 6181 7158 1203 Size: 3171 1807 977 1327 Fragments arranged by size: 3171 1807 1327 977 AgeI A'CCGG_T Cuts at: 6113 6113 Size: 7282 AhdI GACnn_n'nnGTC Cuts at: 2884 3844 6011 2884 Size: 960 2167 4155 Fragments arranged by size: 4155 2167 960 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7282 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2020 2052 2020 Size: 32 7250 AlwNI CAG_nnn'CTG Cuts at: 3085 3499 3953 4228 5025 6597 3085 Size: 414 454 275 797 1572 3770 Fragments arranged by size: 3770 1572 797 454 414 275 ApaI G_GGCC'C Cuts at: 2177 2410 3605 4490 2177 Size: 233 1195 885 4969 Fragments arranged by size: 4969 1195 885 233 ApaLI G'TGCA_C Cuts at: 2542 5339 6495 2542 Size: 2797 1156 3329 Fragments arranged by size: 3329 2797 1156 ApoI r'AATT_y Cuts at: 1493 4591 4817 4969 7078 1493 Size: 3098 226 152 2109 1697 Fragments arranged by size: 3098 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5836 160 Size: 872 4804 1606 Fragments arranged by size: 4804 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 AvaI C'yCGr_G Cuts at: 1870 1943 2117 3435 3864 3918 4607 1870 Size: 73 174 1318 429 54 689 4545 Fragments arranged by size: 4545 1318 689 429 174 73 54 AvrII C'CTAG_G Cuts at: 3742 3742 Size: 7282 BamHI G'GATC_C Cuts at: 3895 4612 4979 3895 Size: 717 367 6198 Fragments arranged by size: 6198 717 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3405 4603 6058 618 Size: 325 217 627 1618 1198 1455 1842 Fragments arranged by size: 1842 1618 1455 1198 627 325 217 BanII G_rGCy'C Cuts at: 1391 2177 2402 2410 3261 3605 3947 4391 4490 Size: 786 225 8 851 344 342 444 99 Cuts at: 4490 4601 1391 Size: 111 4072 Fragments arranged by size: 4072 851 786 444 344 342 225 111 99 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2355 2413 3816 928 Size: 971 456 58 1403 4394 Fragments arranged by size: 4394 1403 971 456 58 BbvCI CC'TCA_GC Cuts at: 2950 3106 3757 2950 Size: 156 651 6475 Fragments arranged by size: 6475 651 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2226 2260 5473 5507 1903 Size: 34 289 34 3213 34 3678 Fragments arranged by size: 3678 3213 289 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5174 6384 5174 Size: 1210 6072 BclI T'GATC_A Cuts at: 1548 1548 Size: 7282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2253 2280 2834 Size: 107 122 71 631 605 581 27 554 Cuts at: 2834 3738 3767 5893 136 Size: 904 29 2126 1525 Fragments arranged by size: 2126 1525 904 631 605 581 554 122 107 71 29 27 BglII A'GATC_T Cuts at: 2757 7277 2757 Size: 4520 2762 BlpI GC'TnA_GC Cuts at: 3946 4699 3946 Size: 753 6529 Bme1580I G_kGCm'C Cuts at: 1790 2177 2410 2546 3000 3410 3605 4490 5343 Size: 387 233 136 454 410 195 885 853 Cuts at: 5343 6499 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 885 853 454 410 387 233 195 136 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4305 5013 5971 Size: 416 53 83 399 24 2888 708 958 Cuts at: 5971 442 Size: 1753 Fragments arranged by size: 2888 1753 958 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7282 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4236 4236 Size: 7282 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2986 3323 3738 3990 4563 5942 1239 Size: 1747 337 415 252 573 1379 2579 Fragments arranged by size: 2579 1747 1379 573 415 337 252 Bpu10I CC'TnA_GC Cuts at: 2401 2950 3106 3377 3757 2401 Size: 549 156 271 380 5926 Fragments arranged by size: 5926 549 380 271 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 2699 3344 3515 5407 6272 6570 2288 Size: 411 645 171 1892 865 298 3000 Fragments arranged by size: 3000 1892 865 645 411 298 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 2463 5945 882 Size: 617 516 448 3482 2219 Fragments arranged by size: 3482 2219 617 516 448 BsaAI yAC'GTr Cuts at: 493 1204 5073 493 Size: 711 3869 2702 Fragments arranged by size: 3869 2702 711 BsaBI GATnn'nnATC Cuts at: 7276 7276 Size: 7282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2132 5089 5471 6106 Size: 53 83 186 936 599 2957 382 635 Cuts at: 6106 275 Size: 1451 Fragments arranged by size: 2957 1451 936 635 599 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5715 6113 6387 6534 6945 5715 Size: 398 274 147 411 6052 Fragments arranged by size: 6052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2020 2050 2536 2566 3613 Size: 30 165 30 617 30 486 30 1047 Cuts at: 3613 3643 4364 4394 1178 Size: 30 721 30 4066 Fragments arranged by size: 4066 1047 721 617 486 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2459 3259 3262 3269 3993 4181 1186 Size: 1273 800 3 7 724 188 4287 Fragments arranged by size: 4287 1273 800 724 188 7 3 BseYI C'CCAG_C Cuts at: 2189 3890 4082 4089 4102 4442 4564 6485 6981 Size: 1701 192 7 13 340 122 1921 496 Cuts at: 6981 2189 Size: 2490 Fragments arranged by size: 2490 1921 1701 496 340 192 122 13 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2095 2669 2675 3164 3200 4092 1163 Size: 573 359 574 6 489 36 892 4353 Fragments arranged by size: 4353 892 574 573 489 359 36 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4732 5493 6521 1540 Size: 275 186 2731 761 1028 2301 Fragments arranged by size: 2731 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2334 2546 2801 3261 3288 4382 5343 5428 6499 Size: 212 255 460 27 1094 961 85 1071 Cuts at: 6499 2334 Size: 3117 Fragments arranged by size: 3117 1094 1071 961 460 255 212 85 27 BsmI GAATG_Cn' Cuts at: 4793 4886 4793 Size: 93 7189 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2334 2402 2410 2546 2801 3000 Size: 399 387 157 68 8 136 255 199 Cuts at: 3000 3261 3288 3410 3605 3947 4382 4391 4490 Size: 261 27 122 195 342 435 9 99 Cuts at: 4490 4601 5343 5428 6499 1391 Size: 111 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 387 342 261 255 199 195 157 136 122 111 99 85 68 27 9 8 BspEI T'CCGG_A Cuts at: 6945 6945 Size: 7282 BspHI T'CATG_A Cuts at: 3799 5171 6901 3799 Size: 1372 1730 4180 Fragments arranged by size: 4180 1730 1372 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2394 2691 2697 3687 3849 4637 844 Size: 1550 297 6 990 162 788 3489 Fragments arranged by size: 3489 1550 990 788 297 162 6 BsrBI CCG'CTC Cuts at: 1394 1823 3400 4351 4363 4483 5169 1394 Size: 429 1577 951 12 120 686 3507 Fragments arranged by size: 3507 1577 951 686 429 120 12 BsrDI GCAATG_nn' Cuts at: 65 1248 2860 3099 5777 5951 65 Size: 1183 1612 239 2678 174 1396 Fragments arranged by size: 2678 1612 1396 1183 239 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5926 6113 1700 Size: 110 4116 187 2869 Fragments arranged by size: 4116 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4116 96 Size: 4020 3262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7282 BssSI C'ACGA_G Cuts at: 5342 6354 5342 Size: 1012 6270 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7282 BstBI TT'CG_AA Cuts at: 6086 6086 Size: 7282 BstEII G'GTnAC_C Cuts at: 1184 2193 1184 Size: 1009 6273 BstXI CCAn_nnnn'nTGG Cuts at: 3716 3716 Size: 7282 BstYI r'GATC_y Cuts at: 1972 2710 2757 2925 3024 3081 3895 4612 4979 Size: 738 47 168 99 57 814 717 367 Cuts at: 4979 5364 5381 6833 6919 6931 7277 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 814 738 717 385 367 346 168 99 86 57 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2841 3597 4060 2841 Size: 756 463 6063 Fragments arranged by size: 6063 756 463 BtgI C'CryG_G Cuts at: 513 1065 2004 2437 2655 3628 3787 513 Size: 552 939 433 218 973 159 4008 Fragments arranged by size: 4008 973 939 552 433 218 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2647 487 Size: 683 56 1421 5122 Fragments arranged by size: 5122 1421 683 56 BtsI GCAGTG_nn' Cuts at: 2249 2263 2396 4109 4798 5592 5612 2249 Size: 14 133 1713 689 794 20 3919 Fragments arranged by size: 3919 1713 794 689 133 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7247 DraI TTT'AAA Cuts at: 4586 4586 Size: 7282 DraIII CAC_nnn'GTG Cuts at: 3938 3938 Size: 7282 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6289 809 Size: 507 4973 1802 Fragments arranged by size: 4973 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2617 4352 4729 Size: 54 1425 50 275 18 787 1735 377 Cuts at: 4729 5618 7247 8 Size: 889 1629 43 Fragments arranged by size: 1735 1629 1425 889 787 377 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4729 1537 Size: 275 2917 4090 Fragments arranged by size: 4090 2917 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3222 3473 4158 5212 1353 Size: 1869 251 685 1054 3423 Fragments arranged by size: 3423 1869 1054 685 251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3900 5869 6243 6389 387 Size: 1064 138 2311 1969 374 146 1280 Fragments arranged by size: 2311 1969 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3259 3259 Size: 7282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2528 2986 3323 3440 3738 3990 4240 Size: 393 896 458 337 117 298 252 250 Cuts at: 4240 4344 4401 4563 5345 5942 6729 6997 1239 Size: 104 57 162 782 597 787 268 1524 Fragments arranged by size: 1524 896 787 782 597 458 393 337 298 268 252 250 162 117 104 57 EcoNI CCTnn'n_nnAGG Cuts at: 2069 2357 3227 4008 4020 2069 Size: 288 870 781 12 5331 Fragments arranged by size: 5331 870 781 288 12 EcoO109I rG'GnC_Cy Cuts at: 2125 2173 2272 2362 2406 3207 3439 3601 3602 Size: 48 99 90 44 801 232 162 1 Cuts at: 3602 3676 3739 3752 3861 4013 4056 4086 4486 Size: 74 63 13 109 152 43 30 400 Cuts at: 4486 4487 2125 Size: 1 4920 Fragments arranged by size: 4920 801 400 232 162 152 109 99 90 74 63 48 44 43 30 13 1 1 EcoRI G'AATT_C Cuts at: 4591 4969 4591 Size: 378 6904 EcoRV GAT'ATC Cuts at: 3526 3526 Size: 7282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 7282 FspI TGC'GCA Cuts at: 2084 5788 2084 Size: 3704 3578 HaeII r_GCGC'y Cuts at: 1678 2434 3660 6429 1678 Size: 756 1226 2769 2531 Fragments arranged by size: 2769 2531 1226 756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2702 2734 2870 2902 3089 Size: 32 135 32 1130 32 136 32 187 Cuts at: 3089 3104 3121 3136 3830 3862 3887 3919 4187 Size: 15 17 15 694 32 25 32 268 Cuts at: 4187 4219 4256 4288 4349 4364 4381 4396 5923 Size: 32 37 32 61 15 17 15 1527 Cuts at: 5923 5955 5997 6029 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1527 1130 694 268 187 136 135 61 42 37 32 32 32 32 32 32 32 32 32 32 25 17 17 15 15 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2647 2878 4626 4872 669 Size: 988 281 709 231 1748 246 3079 Fragments arranged by size: 3079 1748 988 709 281 246 231 HindIII A'AGCT_T Cuts at: 748 3390 3467 748 Size: 2642 77 4563 Fragments arranged by size: 4563 2642 77 HpaI GTT'AAC Cuts at: 4872 4872 Size: 7282 KpnI G_GTAC'C Cuts at: 4607 4607 Size: 7282 MfeI C'AATT_G Cuts at: 4881 4881 Size: 7282 MluI A'CGCG_T Cuts at: 7158 7158 Size: 7282 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3100 4563 6396 6580 1305 Size: 1795 1463 1833 184 2007 Fragments arranged by size: 2007 1833 1795 1463 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5460 Fragments arranged by size: 5460 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 2804 5240 5599 5758 518 Size: 648 1404 234 2436 359 159 2042 Fragments arranged by size: 2436 2042 1404 648 359 234 159 MspA1I CmG'CkG Cuts at: 1606 2189 2596 3110 3352 3698 4106 4698 5375 Size: 583 407 514 242 346 408 592 677 Cuts at: 5375 6523 6768 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 592 583 514 408 407 346 245 242 NcoI C'CATG_G Cuts at: 513 1065 2004 2655 513 Size: 552 939 651 5140 Fragments arranged by size: 5140 939 651 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7282 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7282 NotI GC'GGCC_GC Cuts at: 4729 4729 Size: 7282 NspI r_CATG'y Cuts at: 1132 1229 2731 4237 4378 4640 6185 1132 Size: 97 1502 1506 141 262 1545 2229 Fragments arranged by size: 2229 1545 1506 1502 262 141 97 PasI CC'CwG_GG Cuts at: 1348 2177 2410 2587 3734 4384 1348 Size: 829 233 177 1147 650 4246 Fragments arranged by size: 4246 1147 829 650 233 177 PciI A'CATG_T Cuts at: 4374 6181 4374 Size: 1807 5475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2961 4277 1108 Size: 1853 1316 4113 Fragments arranged by size: 4113 1853 1316 PfoI T'CCnGG_A Cuts at: 2379 2379 Size: 7282 PmeI GTTT'AAAC Cuts at: 4586 4586 Size: 7282 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2020 2052 5351 5383 6864 6896 2020 Size: 32 3299 32 1481 32 2406 Fragments arranged by size: 3299 2406 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2362 3207 3676 3752 3861 4056 4086 2125 Size: 237 845 469 76 109 195 30 5321 Fragments arranged by size: 5321 845 469 237 195 109 76 30 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7282 PsiI TTA'TAA Cuts at: 4852 4852 Size: 7282 PspOMI G'GGCC_C Cuts at: 2173 2406 3601 4486 2173 Size: 233 1195 885 4969 Fragments arranged by size: 4969 1195 885 233 PstI C_TGCA'G Cuts at: 830 2151 2244 2391 4634 830 Size: 1321 93 147 2243 3478 Fragments arranged by size: 3478 2243 1321 147 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7282 PvuII CAG'CTG Cuts at: 2189 2596 3110 3352 3698 4106 2189 Size: 407 514 242 346 408 5365 Fragments arranged by size: 5365 514 408 407 346 242 SacI G_AGCT'C Cuts at: 3261 3261 Size: 7282 SalI G'TCGA_C Cuts at: 1936 2876 4624 1936 Size: 940 1748 4594 Fragments arranged by size: 4594 1748 940 SanDI GG'GwC_CC Cuts at: 3752 3861 4086 3752 Size: 109 225 6948 Fragments arranged by size: 6948 225 109 SbfI CC_TGCA'GG Cuts at: 4634 4634 Size: 7282 ScaI AGT'ACT Cuts at: 5530 7242 5530 Size: 1712 5570 SfcI C'TryA_G Cuts at: 826 1045 2147 2240 2387 4630 5765 6446 6637 Size: 219 1102 93 147 2243 1135 681 191 Cuts at: 6637 826 Size: 1471 Fragments arranged by size: 2243 1471 1135 1102 681 219 191 147 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7282 SmaI CCC'GGG Cuts at: 1872 3437 3866 3920 4609 1872 Size: 1565 429 54 689 4545 Fragments arranged by size: 4545 1565 689 429 54 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 2714 3359 3530 5386 6287 Size: 197 926 324 447 645 171 1856 901 Cuts at: 6287 6549 820 Size: 262 1553 Fragments arranged by size: 1856 1553 926 901 645 447 324 262 197 171 SnaBI TAC'GTA Cuts at: 493 5073 493 Size: 4580 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7282 SphI G_CATG'C Cuts at: 1132 4640 1132 Size: 3508 3774 SspI AAT'ATT Cuts at: 5 52 5206 5 Size: 47 5154 2081 Fragments arranged by size: 5154 2081 47 StuI AGG'CCT Cuts at: 1517 3414 3770 1517 Size: 1897 356 5029 Fragments arranged by size: 5029 1897 356 StyI C'CwwG_G Cuts at: 513 1065 2004 2655 2778 3660 3742 4721 513 Size: 552 939 651 123 882 82 979 3074 Fragments arranged by size: 3074 979 939 882 651 552 123 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2383 2561 2737 3579 5309 5326 5479 Size: 1255 803 178 176 842 1730 17 153 Cuts at: 5479 5664 6109 325 Size: 185 445 1498 Fragments arranged by size: 1730 1498 1255 842 803 445 185 178 176 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3115 4116 5528 Size: 274 80 33 51 1443 1138 1001 1412 Cuts at: 5528 7240 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1412 1138 1001 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2375 2407 2536 2568 3613 3645 7242 7274 2375 Size: 32 129 32 1045 32 3597 32 2383 Fragments arranged by size: 3597 2383 1045 129 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3538 3621 1316 Size: 2222 83 4977 Fragments arranged by size: 4977 2222 83 XbaI T'CTAG_A Cuts at: 4618 4618 Size: 7282 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2255 2086 Size: 169 7113 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7282 XmaI C'CCGG_G Cuts at: 1870 3435 3864 3918 4607 1870 Size: 1565 429 54 689 4545 Fragments arranged by size: 4545 1565 689 429 54 XmnI GAAnn'nnTTC Cuts at: 5411 5411 Size: 7282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5090 276 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI