(Linear) (Six-base) MAPSORT of: KIEE2118.seq Check: 2924 from: 1 to: 2577 KIEE2118.orf With 182 enzymes: SgfI * March 22, 2012 15:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 692 2577 Size: 692 1885 AccI GT'mk_AC Cuts at: 0 55 872 2177 2577 Size: 55 817 1305 400 Fragments arranged by size: 1305 817 400 55 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 1435 2235 2339 2396 2577 Size: 523 912 800 104 57 181 Fragments arranged by size: 912 800 523 181 104 57 AflIII A'CryG_T Cuts at: 0 2369 2577 Size: 2369 208 AhdI GACnn_n'nnGTC Cuts at: 0 879 1839 2577 Size: 879 960 738 Fragments arranged by size: 960 879 738 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 15 47 2577 Size: 15 32 2530 Fragments arranged by size: 2530 32 15 AlwNI CAG_nnn'CTG Cuts at: 0 1080 1494 1948 2223 2577 Size: 1080 414 454 275 354 Fragments arranged by size: 1080 454 414 354 275 ApaI G_GGCC'C Cuts at: 0 172 405 1600 2485 2577 Size: 172 233 1195 885 92 Fragments arranged by size: 1195 885 233 172 92 ApaLI G'TGCA_C Cuts at: 0 537 2577 Size: 537 2040 AvaI C'yCGr_G Cuts at: 0 112 1430 1859 1913 2577 Size: 112 1318 429 54 664 Fragments arranged by size: 1318 664 429 112 54 AvrII C'CTAG_G Cuts at: 0 1737 2577 Size: 1737 840 BamHI G'GATC_C Cuts at: 0 1890 2577 Size: 1890 687 BanI G'GyrC_C Cuts at: 0 1400 2577 Size: 1400 1177 BanII G_rGCy'C Cuts at: 0 172 397 405 1256 1600 1942 2386 2485 Size: 172 225 8 851 344 342 444 99 Cuts at: 2485 2577 Size: 92 Fragments arranged by size: 851 444 344 342 225 172 99 92 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 350 408 1811 2577 Size: 350 58 1403 766 Fragments arranged by size: 1403 766 350 58 BbvCI CC'TCA_GC Cuts at: 0 945 1101 1752 2577 Size: 945 156 651 825 Fragments arranged by size: 945 825 651 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 221 255 2577 Size: 221 34 2322 Fragments arranged by size: 2322 221 34 BglI GCCn_nnn'nGGC Cuts at: 0 248 275 829 1733 1762 2577 Size: 248 27 554 904 29 815 Fragments arranged by size: 904 815 554 248 29 27 BglII A'GATC_T Cuts at: 0 752 2577 Size: 752 1825 BlpI GC'TnA_GC Cuts at: 0 1941 2577 Size: 1941 636 Bme1580I G_kGCm'C Cuts at: 0 172 405 541 995 1405 1600 2485 2577 Size: 172 233 136 454 410 195 885 92 Fragments arranged by size: 885 454 410 233 195 172 136 92 BmrI ACTGGGnnnn_n' Cuts at: 0 2300 2577 Size: 2300 277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2231 2577 Size: 2231 346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 981 1318 1733 1985 2577 Size: 981 337 415 252 592 Fragments arranged by size: 981 592 415 337 252 Bpu10I CC'TnA_GC Cuts at: 0 396 945 1101 1372 1752 2577 Size: 396 549 156 271 380 825 Fragments arranged by size: 825 549 396 380 271 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 694 1339 1510 2577 Size: 283 411 645 171 1067 Fragments arranged by size: 1067 645 411 283 171 BsaI GGTCTCn'nnnn_ Cuts at: 0 10 458 2577 Size: 10 448 2119 Fragments arranged by size: 2119 448 10 BsaHI Gr'CG_yC Cuts at: 0 127 2577 Size: 127 2450 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 15 45 531 561 1608 1638 2359 2389 Size: 15 30 486 30 1047 30 721 30 Cuts at: 2389 2577 Size: 188 Fragments arranged by size: 1047 721 486 188 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 454 1254 1257 1264 1988 2176 2577 Size: 454 800 3 7 724 188 401 Fragments arranged by size: 800 724 454 401 188 7 3 BseYI C'CCAG_C Cuts at: 0 184 1885 2077 2084 2097 2437 2559 2577 Size: 184 1701 192 7 13 340 122 18 Fragments arranged by size: 1701 340 192 184 122 18 13 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 90 664 670 1159 1195 2087 2577 Size: 90 574 6 489 36 892 490 Fragments arranged by size: 892 574 490 489 90 36 6 BsiHKAI G_wGCw'C Cuts at: 0 329 541 796 1256 1283 2377 2577 Size: 329 212 255 460 27 1094 200 Fragments arranged by size: 1094 460 329 255 212 200 27 Bsp1286I G_dGCh'C Cuts at: 0 172 329 397 405 541 796 995 1256 Size: 172 157 68 8 136 255 199 261 Cuts at: 1256 1283 1405 1600 1942 2377 2386 2485 2577 Size: 27 122 195 342 435 9 99 92 Fragments arranged by size: 435 342 261 255 199 195 172 157 136 122 99 92 68 27 9 8 BspHI T'CATG_A Cuts at: 0 1794 2577 Size: 1794 783 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 389 686 692 1682 1844 2577 Size: 389 297 6 990 162 733 Fragments arranged by size: 990 733 389 297 162 6 BsrBI CCG'CTC Cuts at: 0 1395 2346 2358 2478 2577 Size: 1395 951 12 120 99 Fragments arranged by size: 1395 951 120 99 12 BsrDI GCAATG_nn' Cuts at: 0 855 1094 2577 Size: 855 239 1483 Fragments arranged by size: 1483 855 239 BsrGI T'GTAC_A Cuts at: 0 2111 2577 Size: 2111 466 BstEII G'GTnAC_C Cuts at: 0 188 2577 Size: 188 2389 BstXI CCAn_nnnn'nTGG Cuts at: 0 1711 2577 Size: 1711 866 BstYI r'GATC_y Cuts at: 0 705 752 920 1019 1076 1890 2577 Size: 705 47 168 99 57 814 687 Fragments arranged by size: 814 705 687 168 99 57 47 Bsu36I CC'TnA_GG Cuts at: 0 836 1592 2055 2577 Size: 836 756 463 522 Fragments arranged by size: 836 756 522 463 BtgI C'CryG_G Cuts at: 0 432 650 1623 1782 2577 Size: 432 218 973 159 795 Fragments arranged by size: 973 795 432 218 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 642 2577 Size: 642 1935 BtsI GCAGTG_nn' Cuts at: 0 244 258 391 2104 2577 Size: 244 14 133 1713 473 Fragments arranged by size: 1713 473 244 133 14 DraIII CAC_nnn'GTG Cuts at: 0 1933 2577 Size: 1933 644 EaeI y'GGCC_r Cuts at: 0 612 2347 2577 Size: 612 1735 230 Fragments arranged by size: 1735 612 230 EarI CTCTTCn'nnn_ Cuts at: 0 1217 1468 2153 2577 Size: 1217 251 685 424 Fragments arranged by size: 1217 685 424 251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1895 2577 Size: 1895 682 EcoICRI GAG'CTC Cuts at: 0 1254 2577 Size: 1254 1323 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 981 1318 1435 1733 1985 2235 2339 Size: 523 458 337 117 298 252 250 104 Cuts at: 2339 2396 2577 Size: 57 181 Fragments arranged by size: 523 458 337 298 252 250 181 117 104 57 EcoNI CCTnn'n_nnAGG Cuts at: 0 64 352 1222 2003 2015 2577 Size: 64 288 870 781 12 562 Fragments arranged by size: 870 781 562 288 64 12 EcoO109I rG'GnC_Cy Cuts at: 0 120 168 267 357 401 1202 1434 1596 Size: 120 48 99 90 44 801 232 162 Cuts at: 1596 1597 1671 1734 1747 1856 2008 2051 2081 Size: 1 74 63 13 109 152 43 30 Cuts at: 2081 2481 2482 2577 Size: 400 1 95 Fragments arranged by size: 801 400 232 162 152 120 109 99 95 90 74 63 48 44 43 30 13 1 1 EcoRV GAT'ATC Cuts at: 0 1521 2577 Size: 1521 1056 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 822 2577 Size: 822 1755 FspI TGC'GCA Cuts at: 0 79 2577 Size: 79 2498 HaeII r_GCGC'y Cuts at: 0 429 1655 2577 Size: 429 1226 922 Fragments arranged by size: 1226 922 429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 697 729 865 897 1084 1099 1116 1131 Size: 697 32 136 32 187 15 17 15 Cuts at: 1131 1825 1857 1882 1914 2182 2214 2251 2283 Size: 694 32 25 32 268 32 37 32 Cuts at: 2283 2344 2359 2376 2391 2577 Size: 61 15 17 15 186 Fragments arranged by size: 697 694 268 187 186 136 61 37 32 32 32 32 32 32 25 17 17 15 15 15 15 HincII GTy'rAC Cuts at: 0 642 873 2577 Size: 642 231 1704 Fragments arranged by size: 1704 642 231 HindIII A'AGCT_T Cuts at: 0 1385 1462 2577 Size: 1385 77 1115 Fragments arranged by size: 1385 1115 77 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1095 2558 2577 Size: 1095 1463 19 Fragments arranged by size: 1463 1095 19 MslI CAynn'nnrTG Cuts at: 0 565 799 2577 Size: 565 234 1778 Fragments arranged by size: 1778 565 234 MspA1I CmG'CkG Cuts at: 0 184 591 1105 1347 1693 2101 2577 Size: 184 407 514 242 346 408 476 Fragments arranged by size: 514 476 408 407 346 242 184 NcoI C'CATG_G Cuts at: 0 650 2577 Size: 650 1927 NspI r_CATG'y Cuts at: 0 726 2232 2373 2577 Size: 726 1506 141 204 Fragments arranged by size: 1506 726 204 141 PasI CC'CwG_GG Cuts at: 0 172 405 582 1729 2379 2577 Size: 172 233 177 1147 650 198 Fragments arranged by size: 1147 650 233 198 177 172 PciI A'CATG_T Cuts at: 0 2369 2577 Size: 2369 208 PflMI CCAn_nnn'nTGG Cuts at: 0 956 2272 2577 Size: 956 1316 305 Fragments arranged by size: 1316 956 305 PfoI T'CCnGG_A Cuts at: 0 374 2577 Size: 374 2203 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 15 47 2577 Size: 15 32 2530 Fragments arranged by size: 2530 32 15 PpuMI rG'GwC_Cy Cuts at: 0 120 357 1202 1671 1747 1856 2051 2081 Size: 120 237 845 469 76 109 195 30 Cuts at: 2081 2577 Size: 496 Fragments arranged by size: 845 496 469 237 195 120 109 76 30 PspOMI G'GGCC_C Cuts at: 0 168 401 1596 2481 2577 Size: 168 233 1195 885 96 Fragments arranged by size: 1195 885 233 168 96 PstI C_TGCA'G Cuts at: 0 146 239 386 2577 Size: 146 93 147 2191 Fragments arranged by size: 2191 147 146 93 PvuII CAG'CTG Cuts at: 0 184 591 1105 1347 1693 2101 2577 Size: 184 407 514 242 346 408 476 Fragments arranged by size: 514 476 408 407 346 242 184 SacI G_AGCT'C Cuts at: 0 1256 2577 Size: 1256 1321 SalI G'TCGA_C Cuts at: 0 871 2577 Size: 871 1706 SanDI GG'GwC_CC Cuts at: 0 1747 1856 2081 2577 Size: 1747 109 225 496 Fragments arranged by size: 1747 496 225 109 SfcI C'TryA_G Cuts at: 0 142 235 382 2577 Size: 142 93 147 2195 Fragments arranged by size: 2195 147 142 93 SmaI CCC'GGG Cuts at: 0 1432 1861 1915 2577 Size: 1432 429 54 662 Fragments arranged by size: 1432 662 429 54 SmlI C'TyrA_G Cuts at: 0 262 709 1354 1525 2577 Size: 262 447 645 171 1052 Fragments arranged by size: 1052 645 447 262 171 StuI AGG'CCT Cuts at: 0 1409 1765 2577 Size: 1409 356 812 Fragments arranged by size: 1409 812 356 StyI C'CwwG_G Cuts at: 0 650 773 1655 1737 2577 Size: 650 123 882 82 840 Fragments arranged by size: 882 840 650 123 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 378 556 732 1574 2577 Size: 378 178 176 842 1003 Fragments arranged by size: 1003 842 378 178 176 TatI w'GTAC_w Cuts at: 0 1110 2111 2577 Size: 1110 1001 466 Fragments arranged by size: 1110 1001 466 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 370 402 531 563 1608 1640 2577 Size: 370 32 129 32 1045 32 937 Fragments arranged by size: 1045 937 370 129 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1533 1616 2577 Size: 1533 83 961 Fragments arranged by size: 1533 961 83 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 81 250 2577 Size: 81 169 2327 Fragments arranged by size: 2327 169 81 XmaI C'CCGG_G Cuts at: 0 1430 1859 1913 2577 Size: 1430 429 54 664 Fragments arranged by size: 1430 664 429 54 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AloI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII MmeI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SalI SanDI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI AlfI ApoI AscI AseI AsiSI BaeI BbeI BciVI BclI BfrBI BmgBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI DrdI EagI EcoRI FseI FspAI HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI