(Linear) (Six-base) MAPSORT of: KIBB5989.seq Check: 5169 from: 1 to: 1719 KIBB5989.orf With 182 enzymes: SgfI * July 3, 2012 16:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 331 1719 Size: 331 1388 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1641 1719 Size: 1641 78 AfeI AGC'GCT Cuts at: 0 955 1719 Size: 955 764 AleI CACnn'nnGTG Cuts at: 0 40 1719 Size: 40 1679 AlwNI CAG_nnn'CTG Cuts at: 0 176 575 597 890 933 1429 1512 1719 Size: 176 399 22 293 43 496 83 207 Fragments arranged by size: 496 399 293 207 176 83 43 22 ApaI G_GGCC'C Cuts at: 0 824 1719 Size: 824 895 AvaI C'yCGr_G Cuts at: 0 287 1719 Size: 287 1432 BanI G'GyrC_C Cuts at: 0 1144 1161 1719 Size: 1144 17 558 Fragments arranged by size: 1144 558 17 BanII G_rGCy'C Cuts at: 0 146 294 385 824 856 1172 1448 1719 Size: 146 148 91 439 32 316 276 271 Fragments arranged by size: 439 316 276 271 148 146 91 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 1460 1598 1719 Size: 1460 138 121 Fragments arranged by size: 1460 138 121 BbvCI CC'TCA_GC Cuts at: 0 1438 1637 1691 1719 Size: 1438 199 54 28 Fragments arranged by size: 1438 199 54 28 BglI GCCn_nnn'nGGC Cuts at: 0 688 1321 1719 Size: 688 633 398 Fragments arranged by size: 688 633 398 BglII A'GATC_T Cuts at: 0 996 1719 Size: 996 723 Bme1580I G_kGCm'C Cuts at: 0 216 824 1719 Size: 216 608 895 Fragments arranged by size: 895 608 216 BmrI ACTGGGnnnn_n' Cuts at: 0 124 870 900 1339 1719 Size: 124 746 30 439 380 Fragments arranged by size: 746 439 380 124 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 359 597 1719 Size: 110 249 238 1122 Fragments arranged by size: 1122 249 238 110 Bpu10I CC'TnA_GC Cuts at: 0 602 1438 1637 1691 1719 Size: 602 836 199 54 28 Fragments arranged by size: 836 602 199 54 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1517 1719 Size: 1517 202 BsaI GGTCTCn'nnnn_ Cuts at: 0 1043 1719 Size: 1043 676 BsaAI yAC'GTr Cuts at: 0 1567 1719 Size: 1567 152 BsaHI Gr'CG_yC Cuts at: 0 1386 1719 Size: 1386 333 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1695 1719 Size: 1695 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 362 457 460 964 1395 1719 Size: 362 95 3 504 431 324 Fragments arranged by size: 504 431 362 324 95 3 BseYI C'CCAG_C Cuts at: 0 298 453 483 1122 1719 Size: 298 155 30 639 597 Fragments arranged by size: 639 597 298 155 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1393 1719 Size: 1393 326 BsiHKAI G_wGCw'C Cuts at: 0 146 1019 1073 1172 1448 1719 Size: 146 873 54 99 276 271 Fragments arranged by size: 873 276 271 146 99 54 Bsp1286I G_dGCh'C Cuts at: 0 146 216 294 385 824 856 1019 1073 Size: 146 70 78 91 439 32 163 54 Cuts at: 1073 1172 1448 1719 Size: 99 276 271 Fragments arranged by size: 439 276 271 163 146 99 91 78 70 54 32 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 331 1719 Size: 331 1388 BsrDI GCAATG_nn' Cuts at: 0 354 1719 Size: 354 1365 BsrGI T'GTAC_A Cuts at: 0 1500 1719 Size: 1500 219 BstAPI GCAn_nnn'nTGC Cuts at: 0 597 1719 Size: 597 1122 BstXI CCAn_nnnn'nTGG Cuts at: 0 42 438 1406 1719 Size: 42 396 968 313 Fragments arranged by size: 968 396 313 42 BstYI r'GATC_y Cuts at: 0 188 996 1719 Size: 188 808 723 Fragments arranged by size: 808 723 188 BtsI GCAGTG_nn' Cuts at: 0 650 782 1376 1719 Size: 650 132 594 343 Fragments arranged by size: 650 594 343 132 DrdI GACnn_nn'nnGTC Cuts at: 0 769 1719 Size: 769 950 EaeI y'GGCC_r Cuts at: 0 804 966 1719 Size: 804 162 753 Fragments arranged by size: 804 753 162 EarI CTCTTCn'nnn_ Cuts at: 0 322 1719 Size: 322 1397 EcoICRI GAG'CTC Cuts at: 0 144 1170 1446 1719 Size: 144 1026 276 273 Fragments arranged by size: 1026 276 273 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 359 597 1641 1719 Size: 110 249 238 1044 78 Fragments arranged by size: 1044 249 238 110 78 EcoNI CCTnn'n_nnAGG Cuts at: 0 374 925 1719 Size: 374 551 794 Fragments arranged by size: 794 551 374 EcoO109I rG'GnC_Cy Cuts at: 0 84 406 799 821 930 1672 1719 Size: 84 322 393 22 109 742 47 Fragments arranged by size: 742 393 322 109 84 47 22 HaeII r_GCGC'y Cuts at: 0 957 1413 1512 1719 Size: 957 456 99 207 Fragments arranged by size: 957 456 207 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1092 1124 1216 1248 1371 1403 1719 Size: 1092 32 92 32 123 32 316 Fragments arranged by size: 1092 316 123 92 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1239 1332 1719 Size: 1239 93 387 Fragments arranged by size: 1239 387 93 MscI TGG'CCA Cuts at: 0 968 1719 Size: 968 751 MslI CAynn'nnrTG Cuts at: 0 40 436 1719 Size: 40 396 1283 Fragments arranged by size: 1283 396 40 MspA1I CmG'CkG Cuts at: 0 40 161 176 663 1126 1268 1492 1641 Size: 40 121 15 487 463 142 224 149 Cuts at: 1641 1719 Size: 78 Fragments arranged by size: 487 463 224 149 142 121 78 40 15 PasI CC'CwG_GG Cuts at: 0 385 402 927 1719 Size: 385 17 525 792 Fragments arranged by size: 792 525 385 17 PflMI CCAn_nnn'nTGG Cuts at: 0 890 933 1719 Size: 890 43 786 Fragments arranged by size: 890 786 43 PfoI T'CCnGG_A Cuts at: 0 626 1681 1719 Size: 626 1055 38 Fragments arranged by size: 1055 626 38 PmlI CAC'GTG Cuts at: 0 1567 1719 Size: 1567 152 PpuMI rG'GwC_Cy Cuts at: 0 406 799 1719 Size: 406 393 920 Fragments arranged by size: 920 406 393 PshAI GACnn'nnGTC Cuts at: 0 281 1292 1719 Size: 281 1011 427 Fragments arranged by size: 1011 427 281 PspOMI G'GGCC_C Cuts at: 0 820 1719 Size: 820 899 PstI C_TGCA'G Cuts at: 0 593 1719 Size: 593 1126 PvuII CAG'CTG Cuts at: 0 40 663 1126 1492 1719 Size: 40 623 463 366 227 Fragments arranged by size: 623 463 366 227 40 SacI G_AGCT'C Cuts at: 0 146 1172 1448 1719 Size: 146 1026 276 271 Fragments arranged by size: 1026 276 271 146 SanDI GG'GwC_CC Cuts at: 0 406 1719 Size: 406 1313 SexAI A'CCwGG_T Cuts at: 0 978 1719 Size: 978 741 SfcI C'TryA_G Cuts at: 0 168 508 522 589 754 1192 1629 1719 Size: 168 340 14 67 165 438 437 90 Fragments arranged by size: 438 437 340 168 165 90 67 14 SmlI C'TyrA_G Cuts at: 0 1532 1719 Size: 1532 187 StuI AGG'CCT Cuts at: 0 691 752 1284 1635 1719 Size: 691 61 532 351 84 Fragments arranged by size: 691 532 351 84 61 StyI C'CwwG_G Cuts at: 0 553 848 1064 1364 1520 1719 Size: 553 295 216 300 156 199 Fragments arranged by size: 553 300 295 216 199 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1162 1719 Size: 1162 557 TatI w'GTAC_w Cuts at: 0 878 1500 1597 1719 Size: 878 622 97 122 Fragments arranged by size: 878 622 122 97 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1289 1321 1719 Size: 1289 32 398 Fragments arranged by size: 1289 398 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 301 1050 1719 Size: 301 749 669 Fragments arranged by size: 749 669 301 Enzymes that do cut: AarI AcuI AfeI AleI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstAPI BstXI BstYI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SexAI SfcI SmlI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI