################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI DraI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 217 GTATAC GT^[AC][GT]AC AccIII 794 TCCGGA T^CCGGA AcsI 775 GAATTT [GA]^AATT[CT] AcyI 233 GGCGCC G[GA]^CG[CT]C AlwNI 526 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1471 CCTGAGG CC^T[ACGT]AGG ApoI 775 GAATTT [GA]^AATT[CT] Asp700I 1347 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 233 GGCGCC G^G[CT][GA]CC BbeI 233 GGCGCC GGCGC^C BbvCI 558 GCTGAGG CCTCAGC(-5/-2) BcgI 600 GCAAGAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 83 AGATCT A^GATCT BsaHI 233 GGCGCC G[GA]^CG[CT]C BsaWI 794 TCCGGA [AT]^CCGG[AT] BseAI 794 TCCGGA T^CCGGA BsgI 986 GTGCAG GTGCAG(16/14) BsmI 7 GCATTC GAATGC(1/-1) BspEI 794 TCCGGA T^CCGGA BsrBI 1000 GAGCGG CCGCTC(-3/-3) BsrDI 948 CATTGC GCAATG(2/0) BssSI 1375 CTCGTG CACGAG(-5/-1) Bst1107I 217 GTATAC GTA^TAC BstAPI 525 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 217 GTATAC GTA^TAC Bsu36I 1471 CCTGAGG CC^T[ACGT]AGG DraIII 317 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 426 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1471 CCTGAGG CC^T[ACGT]AGG EcoT14I 237 CCAAGG C^C[AT][AT]GG EcoT22I 5 ATGCAT ATGCA^T Hin1I 233 GGCGCC G[GA]^CG[CT]C KasI 233 GGCGCC G^GCGCC MroI 794 TCCGGA T^CCGGA MslI 813 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 233 GGCGCC GG^CGCC NsiI 5 ATGCAT ATGCA^T NspI 716 GCATGT [GA]CATG^[CT] PaeR7I 1050 CTCGAG C^TCGAG SapI 1105 GAAGAGC GCTCTTC(1/4) SexAI 206 ACCAGGT A^CC[AT]GGT SfoI 233 GGCGCC GGC^GCC SmaI 740 CCCGGG CCC^GGG StuI 1119 AGGCCT AGG^CCT StyI 237 CCAAGG C^C[AT][AT]GG Tsp509I 776 AATT ^AATT XcmI 815 CCATTATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1050 CTCGAG C^TCGAG XmaCI 740 CCCGGG C^CCGGG XmaI 740 CCCGGG C^CCGGG XmnI 1347 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 551 GGGCCC GGGCC^C 1243 GGGCCC AspHI 370 GAGCAC G[AT]GC[AT]^C 911 GAGCAC BlpI 1147 GCTCAGC GC^T[ACGT]AGC 1316 GCTCAGC Bpu1102I 1147 GCTCAGC GC^T[ACGT]AGC 1316 GCTCAGC BsaI 925 GAGACC GGTCTC(1/5) 22 GGTCTC BsiHKAI 370 GAGCAC G[AT]GC[AT]^C 911 GAGCAC BspMI 977 ACCTGC ACCTGC(4/8) 846 GCAGGT BstYI 83 AGATCT [GA]^GATC[CT] 1341 GGATCT CelII 1147 GCTCAGC GC^T[ACGT]AGC 1316 GCTCAGC CfoI 234 GCGC GCG^C 291 GCGC DraII 551 GGGCCCT [GA]G^G[ACGT]CC[CT] 1243 GGGCCCC EcoO109I 551 GGGCCCT [GA]G^G[ACGT]CC[CT] 1243 GGGCCCC HaeII 233 GGCGCC [GA]GCGC^[CT] 290 AGCGCC HgaI 456 GACGC GACGC(5/10) 767 GACGC HhaI 234 GCGC GCG^C 291 GCGC HinP1I 234 GCGC G^CGC 291 GCGC MflI 83 AGATCT [GA]^GATC[CT] 1341 GGATCT MseI 698 TTAA T^TAA 779 TTAA Tru9I 698 TTAA T^TAA 779 TTAA XhoII 83 AGATCT [GA]^GATC[CT] 1341 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp45I TspRI TthHB8I