(Circular) (Six-base) MAPSORT of: pFN21AE3233.seq Check: 7889 from: 1 to: 6334 pFN21AE3233 6334 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2842 2991 2842 Size: 149 6185 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2223 3677 1937 Size: 286 1454 4594 Fragments arranged by size: 4594 1454 286 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2402 2616 3621 4397 5781 6049 1632 Size: 557 213 214 1005 776 1384 268 1917 Fragments arranged by size: 1917 1384 1005 776 557 268 214 213 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3215 3247 3215 Size: 32 6302 AlwNI CAG_nnn'CTG Cuts at: 2536 4077 5649 2536 Size: 1541 1572 3221 Fragments arranged by size: 3221 1572 1541 ApaI G_GGCC'C Cuts at: 2560 3252 2560 Size: 692 5642 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 2780 3643 3869 4021 6130 1493 Size: 1287 863 226 152 2109 1697 Fragments arranged by size: 2109 1697 1287 863 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2745 3055 3659 1870 Size: 73 80 722 310 604 4545 Fragments arranged by size: 4545 722 604 310 80 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 3655 5110 618 Size: 325 217 627 451 1417 1455 1842 Fragments arranged by size: 1842 1455 1417 627 451 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2063 2560 2886 2927 3252 3653 1391 Size: 633 39 497 326 41 325 401 4072 Fragments arranged by size: 4072 633 497 401 326 325 41 39 BbeI G_GCGC'C Cuts at: 2242 2242 Size: 6334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3349 3501 3575 928 Size: 971 1450 152 74 3687 Fragments arranged by size: 3687 1450 971 152 74 BbvCI CC'TCA_GC Cuts at: 2564 2564 Size: 6334 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2594 2628 4525 4559 1903 Size: 34 657 34 1897 34 3678 Fragments arranged by size: 3678 1897 657 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BfrBI ATG'CAT Cuts at: 2012 2012 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4945 136 Size: 107 122 71 631 605 3273 1525 Fragments arranged by size: 3273 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2088 6329 2088 Size: 4241 2093 BlpI GC'TnA_GC Cuts at: 3153 3322 3751 3153 Size: 169 429 5736 Fragments arranged by size: 5736 429 169 Bme1580I G_kGCm'C Cuts at: 1790 2262 2560 3122 3252 4395 5551 1790 Size: 472 298 562 130 1143 1156 2573 Fragments arranged by size: 2573 1156 1143 562 472 298 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4065 5023 442 Size: 416 53 83 399 24 2648 958 1753 Fragments arranged by size: 2648 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2902 2902 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2852 2858 3023 4994 1239 Size: 838 775 6 165 1971 2579 Fragments arranged by size: 2579 1971 838 775 165 6 Bpu10I CC'TnA_GC Cuts at: 2564 2564 Size: 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 2996 3505 4459 5324 5622 2015 Size: 981 509 954 865 298 2727 Fragments arranged by size: 2727 981 954 865 509 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2033 2924 4997 882 Size: 617 534 891 2073 2219 Fragments arranged by size: 2219 2073 891 617 534 BsaAI yAC'GTr Cuts at: 493 1204 4125 493 Size: 711 2921 2702 Fragments arranged by size: 2921 2702 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2239 4141 4523 5158 Size: 53 83 186 936 706 1902 382 635 Cuts at: 5158 275 Size: 1451 Fragments arranged by size: 1902 1451 936 706 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2799 4767 5165 5439 5586 5997 2799 Size: 1968 398 274 147 411 3136 Fragments arranged by size: 3136 1968 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6109 Fragments arranged by size: 6109 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2894 3104 3160 3494 1186 Size: 1708 210 56 334 4026 Fragments arranged by size: 4026 1708 334 210 56 BseYI C'CCAG_C Cuts at: 2261 2292 2696 2863 3403 3576 5537 6033 2261 Size: 31 404 167 540 173 1961 496 2562 Fragments arranged by size: 2562 1961 540 496 404 173 167 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3012 1163 Size: 573 1276 4485 Fragments arranged by size: 4485 1276 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2379 2920 4395 4480 5551 2379 Size: 541 1475 85 1071 3162 Fragments arranged by size: 3162 1475 1071 541 85 BsmI GAATG_Cn' Cuts at: 2012 3845 3938 2012 Size: 1833 93 4408 Fragments arranged by size: 4408 1833 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2063 2262 2379 2560 2886 2920 Size: 399 234 39 199 117 181 326 34 Cuts at: 2920 2927 3122 3252 3653 4395 4480 5551 1391 Size: 7 195 130 401 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 401 399 326 234 199 195 181 130 117 85 39 34 7 BspEI T'CCGG_A Cuts at: 2799 5997 2799 Size: 3198 3136 BspHI T'CATG_A Cuts at: 4223 5953 4223 Size: 1730 4604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2842 2991 3689 844 Size: 1998 149 698 3489 Fragments arranged by size: 3489 1998 698 149 BsrBI CCG'CTC Cuts at: 1394 1823 3007 4221 1394 Size: 429 1184 1214 3507 Fragments arranged by size: 3507 1214 1184 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2952 4829 5003 65 Size: 1183 1704 1877 174 1396 Fragments arranged by size: 1877 1704 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4978 5165 1700 Size: 110 3168 187 2869 Fragments arranged by size: 3168 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 3380 4394 5406 3380 Size: 1014 1012 4308 Fragments arranged by size: 4308 1014 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2536 794 Size: 1742 4592 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2088 3346 3664 4031 4416 4433 5885 5971 Size: 116 1258 318 367 385 17 1452 86 Cuts at: 5971 5983 6329 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1258 385 367 346 318 116 86 17 12 BstZ17I GTA'TAC Cuts at: 2224 2224 Size: 6334 Bsu36I CC'TnA_GG Cuts at: 3477 3477 Size: 6334 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2104 2139 2979 3636 3850 4644 4664 2104 Size: 35 840 657 214 794 20 3774 Fragments arranged by size: 3774 840 794 657 214 35 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DraIII CAC_nnn'GTG Cuts at: 2327 2327 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2437 5341 809 Size: 507 1121 2904 1802 Fragments arranged by size: 2904 1802 1121 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3781 4670 6299 Size: 54 1425 50 275 18 1951 889 1629 Cuts at: 6299 8 Size: 43 Fragments arranged by size: 1951 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2382 2592 3105 3543 4264 1353 Size: 1029 210 513 438 721 3423 Fragments arranged by size: 3423 1029 721 513 438 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2612 4921 5295 5441 387 Size: 1064 138 1023 2309 374 146 1280 Fragments arranged by size: 2309 1280 1064 1023 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2189 2402 2616 2852 2858 3023 Size: 393 445 112 213 214 236 6 165 Cuts at: 3023 3621 4397 4994 5781 6049 1239 Size: 598 776 597 787 268 1524 Fragments arranged by size: 1524 787 776 598 597 445 393 268 236 214 213 165 112 6 EcoO109I rG'GnC_Cy Cuts at: 2557 3249 2557 Size: 692 5642 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 2242 2299 5481 1678 Size: 564 57 3182 2531 Fragments arranged by size: 3182 2531 564 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2075 2077 2107 2459 Size: 32 135 32 473 30 2 30 352 Cuts at: 2459 2491 2708 2740 4975 5007 5049 5081 1373 Size: 32 217 32 2235 32 42 32 2626 Fragments arranged by size: 2626 2235 473 352 217 135 42 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3678 3924 669 Size: 988 281 1740 246 3079 Fragments arranged by size: 3079 1740 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 2238 2238 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 5448 5632 1305 Size: 792 3351 184 2007 Fragments arranged by size: 3351 2007 792 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4512 Fragments arranged by size: 4512 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 4292 4651 4810 518 Size: 648 1656 1470 359 159 2042 Fragments arranged by size: 2042 1656 1470 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2115 2185 3033 3157 3407 3750 4427 5575 Size: 509 70 848 124 250 343 677 1148 Cuts at: 5575 5820 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 848 677 509 343 250 245 124 70 NarI GG'CG_CC Cuts at: 2239 2239 Size: 6334 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NsiI A_TGCA'T Cuts at: 2014 2014 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2725 3692 5237 1132 Size: 97 1496 967 1545 2229 Fragments arranged by size: 2229 1545 1496 967 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PfoI T'CCnGG_A Cuts at: 2943 2943 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PspOMI G'GGCC_C Cuts at: 2556 3248 2556 Size: 692 5642 PspXI vC'TCGA_Gb Cuts at: 3055 3055 Size: 6334 PstI C_TGCA'G Cuts at: 830 2532 2690 2988 3686 830 Size: 1702 158 298 698 3478 Fragments arranged by size: 3478 1702 698 298 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2115 2185 3157 3407 2115 Size: 70 972 250 5042 Fragments arranged by size: 5042 972 250 70 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SapI GCTCTTCn'nnn_ Cuts at: 3105 3105 Size: 6334 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SexAI A'CCwGG_T Cuts at: 2211 2211 Size: 6334 SfcI C'TryA_G Cuts at: 826 1045 2528 2686 2984 3682 4817 5498 5689 Size: 219 1483 158 298 698 1135 681 191 Cuts at: 5689 826 Size: 1471 Fragments arranged by size: 1483 1471 1135 698 681 298 219 191 158 SfoI GGC'GCC Cuts at: 2240 2240 Size: 6334 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 2747 3661 1872 Size: 875 914 4545 Fragments arranged by size: 4545 914 875 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 3011 3055 3520 4438 5339 Size: 197 926 87 981 44 465 918 901 Cuts at: 5339 5601 820 Size: 262 1553 Fragments arranged by size: 1553 981 926 918 901 465 262 197 87 44 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 3126 1517 Size: 1609 4725 StyI C'CwwG_G Cuts at: 513 1065 2004 2242 3773 513 Size: 552 939 238 1531 3074 Fragments arranged by size: 3074 1531 939 552 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 3062 3219 3454 4361 4378 4531 Size: 1255 590 892 157 235 907 17 153 Cuts at: 4531 4716 5161 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 907 892 590 445 235 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4580 6292 96 Size: 274 80 33 51 1443 2603 1712 138 Fragments arranged by size: 2603 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6294 6326 6294 Size: 32 6302 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2827 2827 Size: 6334 XhoI C'TCGA_G Cuts at: 1943 3055 1943 Size: 1112 5222 XmaI C'CCGG_G Cuts at: 1870 2745 3659 1870 Size: 875 914 4545 Fragments arranged by size: 4545 914 875 XmnI GAAnn'nnTTC Cuts at: 3356 4463 3356 Size: 1107 5227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI