(Linear) (Six-base) MAPSORT of: KIEE3233.seq Check: 4241 from: 1 to: 1629 KIEE3233.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 837 986 1629 Size: 837 149 643 Fragments arranged by size: 837 643 149 AccI GT'mk_AC Cuts at: 0 218 1629 Size: 218 1411 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 397 611 1616 1629 Size: 184 213 214 1005 13 Fragments arranged by size: 1005 214 213 184 13 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1210 1242 1629 Size: 1210 32 387 Fragments arranged by size: 1210 387 32 AlwNI CAG_nnn'CTG Cuts at: 0 531 1629 Size: 531 1098 ApaI G_GGCC'C Cuts at: 0 555 1247 1629 Size: 555 692 382 Fragments arranged by size: 692 555 382 ApoI r'AATT_y Cuts at: 0 775 1629 Size: 775 854 AvaI C'yCGr_G Cuts at: 0 18 740 1050 1629 Size: 18 722 310 579 Fragments arranged by size: 722 579 310 18 BanI G'GyrC_C Cuts at: 0 233 1629 Size: 233 1396 BanII G_rGCy'C Cuts at: 0 19 58 555 881 922 1247 1629 Size: 19 39 497 326 41 325 382 Fragments arranged by size: 497 382 326 325 41 39 19 BbeI G_GCGC'C Cuts at: 0 237 1629 Size: 237 1392 BbsI GAAGACnn'nnnn_ Cuts at: 0 1344 1496 1570 1629 Size: 1344 152 74 59 Fragments arranged by size: 1344 152 74 59 BbvCI CC'TCA_GC Cuts at: 0 559 1629 Size: 559 1070 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 589 623 1629 Size: 589 34 1006 Fragments arranged by size: 1006 589 34 BfrBI ATG'CAT Cuts at: 0 7 1629 Size: 7 1622 BglII A'GATC_T Cuts at: 0 83 1629 Size: 83 1546 BlpI GC'TnA_GC Cuts at: 0 1148 1317 1629 Size: 1148 169 312 Fragments arranged by size: 1148 312 169 Bme1580I G_kGCm'C Cuts at: 0 257 555 1117 1247 1629 Size: 257 298 562 130 382 Fragments arranged by size: 562 382 298 257 130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 897 1629 Size: 897 732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 847 853 1018 1629 Size: 72 775 6 165 611 Fragments arranged by size: 775 611 165 72 6 Bpu10I CC'TnA_GC Cuts at: 0 559 1629 Size: 559 1070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 991 1500 1629 Size: 10 981 509 129 Fragments arranged by size: 981 509 129 10 BsaI GGTCTCn'nnnn_ Cuts at: 0 28 919 1629 Size: 28 891 710 Fragments arranged by size: 891 710 28 BsaHI Gr'CG_yC Cuts at: 0 234 1629 Size: 234 1395 BsaWI w'CCGG_w Cuts at: 0 794 1629 Size: 794 835 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 889 1099 1155 1489 1629 Size: 889 210 56 334 140 Fragments arranged by size: 889 334 210 140 56 BseYI C'CCAG_C Cuts at: 0 256 287 691 858 1398 1571 1629 Size: 256 31 404 167 540 173 58 Fragments arranged by size: 540 404 256 173 167 58 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1007 1629 Size: 1007 622 BsiHKAI G_wGCw'C Cuts at: 0 374 915 1629 Size: 374 541 714 Fragments arranged by size: 714 541 374 BsmI GAATG_Cn' Cuts at: 0 7 1629 Size: 7 1622 Bsp1286I G_dGCh'C Cuts at: 0 19 58 257 374 555 881 915 922 Size: 19 39 199 117 181 326 34 7 Cuts at: 922 1117 1247 1629 Size: 195 130 382 Fragments arranged by size: 382 326 199 195 181 130 117 39 34 19 7 BspEI T'CCGG_A Cuts at: 0 794 1629 Size: 794 835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 837 986 1629 Size: 837 149 643 Fragments arranged by size: 837 643 149 BsrBI CCG'CTC Cuts at: 0 1002 1629 Size: 1002 627 BsrDI GCAATG_nn' Cuts at: 0 947 1629 Size: 947 682 BssSI C'ACGA_G Cuts at: 0 1375 1629 Size: 1375 254 BstAPI GCAn_nnn'nTGC Cuts at: 0 531 1629 Size: 531 1098 BstYI r'GATC_y Cuts at: 0 83 1341 1629 Size: 83 1258 288 Fragments arranged by size: 1258 288 83 BstZ17I GTA'TAC Cuts at: 0 219 1629 Size: 219 1410 Bsu36I CC'TnA_GG Cuts at: 0 1472 1629 Size: 1472 157 BtsI GCAGTG_nn' Cuts at: 0 99 134 974 1629 Size: 99 35 840 655 Fragments arranged by size: 840 655 99 35 DraIII CAC_nnn'GTG Cuts at: 0 322 1629 Size: 322 1307 DrdI GACnn_nn'nnGTC Cuts at: 0 432 1629 Size: 432 1197 EarI CTCTTCn'nnn_ Cuts at: 0 377 587 1100 1538 1629 Size: 377 210 513 438 91 Fragments arranged by size: 513 438 377 210 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 607 1629 Size: 607 1022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 184 397 611 847 853 1018 1616 Size: 72 112 213 214 236 6 165 598 Cuts at: 1616 1629 Size: 13 Fragments arranged by size: 598 236 214 213 165 112 72 13 6 EcoO109I rG'GnC_Cy Cuts at: 0 552 1244 1629 Size: 552 692 385 Fragments arranged by size: 692 552 385 HaeII r_GCGC'y Cuts at: 0 237 294 1629 Size: 237 57 1335 Fragments arranged by size: 1335 237 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 40 70 72 102 454 486 703 735 Size: 40 30 2 30 352 32 217 32 Cuts at: 735 1629 Size: 894 Fragments arranged by size: 894 352 217 40 32 32 30 30 2 KasI G'GCGC_C Cuts at: 0 233 1629 Size: 233 1396 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 92 1629 Size: 92 1537 MslI CAynn'nnrTG Cuts at: 0 817 1629 Size: 817 812 MspA1I CmG'CkG Cuts at: 0 110 180 1028 1152 1402 1629 Size: 110 70 848 124 250 227 Fragments arranged by size: 848 250 227 124 110 70 NarI GG'CG_CC Cuts at: 0 234 1629 Size: 234 1395 NsiI A_TGCA'T Cuts at: 0 9 1629 Size: 9 1620 NspI r_CATG'y Cuts at: 0 720 1629 Size: 720 909 PfoI T'CCnGG_A Cuts at: 0 938 1629 Size: 938 691 PspOMI G'GGCC_C Cuts at: 0 551 1243 1629 Size: 551 692 386 Fragments arranged by size: 692 551 386 PspXI vC'TCGA_Gb Cuts at: 0 1050 1629 Size: 1050 579 PstI C_TGCA'G Cuts at: 0 527 685 983 1629 Size: 527 158 298 646 Fragments arranged by size: 646 527 298 158 PvuII CAG'CTG Cuts at: 0 110 180 1152 1402 1629 Size: 110 70 972 250 227 Fragments arranged by size: 972 250 227 110 70 SapI GCTCTTCn'nnn_ Cuts at: 0 1100 1629 Size: 1100 529 SexAI A'CCwGG_T Cuts at: 0 206 1629 Size: 206 1423 SfcI C'TryA_G Cuts at: 0 523 681 979 1629 Size: 523 158 298 650 Fragments arranged by size: 650 523 298 158 SfoI GGC'GCC Cuts at: 0 235 1629 Size: 235 1394 SmaI CCC'GGG Cuts at: 0 742 1629 Size: 742 887 SmlI C'TyrA_G Cuts at: 0 25 1006 1050 1515 1629 Size: 25 981 44 465 114 Fragments arranged by size: 981 465 114 44 25 StuI AGG'CCT Cuts at: 0 1121 1629 Size: 1121 508 StyI C'CwwG_G Cuts at: 0 237 1629 Size: 237 1392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 165 1057 1214 1449 1629 Size: 165 892 157 235 180 Fragments arranged by size: 892 235 180 165 157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 822 1629 Size: 822 807 XhoI C'TCGA_G Cuts at: 0 1050 1629 Size: 1050 579 XmaI C'CCGG_G Cuts at: 0 740 1629 Size: 740 889 XmnI GAAnn'nnTTC Cuts at: 0 1351 1629 Size: 1351 278 Enzymes that do cut: AarI AccI AcuI AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI BstYI BstZ17I Bsu36I BtsI DraIII DrdI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NarI NsiI NspI PfoI PspOMI PspXI PstI PvuII SapI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BglI BmgBI BmrI BmtI BsaAI BsaBI BsaXI BsiEI BsiWI BsmBI BspHI BsrFI BsrGI BssHII BstBI BstEII BstXI BtgI BtgZI ClaI CspCI DraI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI ZraI