################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AccI AccII AclI AflII AflIII AgeI AlwI AlwNI AocI Aor51HI AscI Asp700I AspI AvaI AviII BamHI BbeI BbvCI BciVI BfrI BsaI BsiWI BsmBI BsmI BsoBI BspDI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FseI FspI HincII HindII HpaI KasI Ksp632I KspI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 78 enzymes) # ################################### AatII 400 GACGTC GACGT^C Acc65I 670 GGTACC G^GTACC AccIII 557 TCCGGA T^CCGGA AcsI 936 GAATTC [GA]^AATT[CT] AhdI 62 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 651 GTGCAC G^TGCAC ApaI 113 GGGCCC GGGCC^C ApaLI 651 GTGCAC G^TGCAC ApoI 936 GAATTC [GA]^AATT[CT] AseI 199 ATTAAT AT^TAAT AsnI 199 ATTAAT AT^TAAT Asp718I 670 GGTACC G^GTACC AspEI 62 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 651 GTGCAC G[AT]GC[AT]^C AvrII 420 CCTAGG C^CTAGG BalI 885 TGGCCA TGG^CCA BanI 670 GGTACC G^G[CT][GA]CC BbrPI 757 CACGTG CAC^GTG BcgI 84 GCAACTTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 497 TGATCA T^GATCA BglI 298 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 645 AGATCT A^GATCT BlnI 420 CCTAGG C^CTAGG BlpI 1015 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1015 GCTGAGC GC^T[ACGT]AGC BsaAI 757 CACGTG [CT]AC^GT[GA] BsaBI 541 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 557 TCCGGA T^CCGGA BseRI 803 CTCCTC GAGGAG(10/8) BsgI 1033 CTGCAC GTGCAG(16/14) BsiEI 28 CGGTCG CG[GA][CT]^CG BsiHKAI 651 GTGCAC G[AT]GC[AT]^C Bsp1407I 254 TGTACA T^GTACA BspEI 557 TCCGGA T^CCGGA BspHI 266 TCATGA T^CATGA BspMI 797 ACCTGC ACCTGC(4/8) BsrGI 254 TGTACA T^GTACA BsrI 855 ACTGG ACTGG(1/-1) BstEII 180 GGTAACC G^GT[ACGT]ACC BstPI 180 GGTAACC G^GT[ACGT]ACC BstXI 449 CCAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 645 AGATCT [GA]^GATC[CT] Cac8I 887 GCCAGC GC[ACGT]^[ACGT]GC CelII 1015 GCTGAGC GC^T[ACGT]AGC DsaI 927 CCGTGG C^C[GA][CT]GG EaeI 885 TGGCCA [CT]^GGCC[GA] Eam1105I 62 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 180 GGTAACC G^GT[ACGT]ACC EcoRI 936 GAATTC G^AATTC FauI 239 CCCGC CCCGC(4/6) FbaI 497 TGATCA T^GATCA HaeII 3 GGCGCT [GA]GCGC^[CT] HgaI 636 GCGTC GACGC(5/10) HindIII 130 AAGCTT A^AGCTT KpnI 670 GGTACC GGTAC^C MamI 541 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 645 AGATCT [GA]^GATC[CT] MluNI 885 TGGCCA TGG^CCA MroI 557 TCCGGA T^CCGGA MscI 885 TGGCCA TGG^CCA MseI 200 TTAA T^TAA MslI 946 CATATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 889 CAGCTG C[AC]G^C[GT]G PflMI 723 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 757 CACGTG CAC^GTG PmlI 757 CACGTG CAC^GTG PshBI 199 ATTAAT AT^TAAT PvuII 889 CAGCTG CAG^CTG RcaI 266 TCATGA T^CATGA SexAI 455 ACCTGGT A^CC[AT]GGT SmlI 768 CTTGAG C^T[CT][GA]AG SspBI 254 TGTACA T^GTACA TaqI 966 TCGA T^CGA TfiI 846 GATTC G^A[AT]TC Tru9I 200 TTAA T^TAA TthHB8I 966 TCGA T^CGA Van91I 723 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 645 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AciI 240 CCGC CCGC(-3/-1) 393 CCGC AcyI 400 GACGTC G[GA]^CG[CT]C 635 GGCGTC AvaII 512 GGACC G^G[AT]CC 1044 GGTCC BfaI 308 CTAG C^TAG 421 CTAG BpmI 9 CTCCAG CTGGAG(16/14) 745 CTCCAG BsaHI 400 GACGTC G[GA]^CG[CT]C 635 GGCGTC BsaWI 557 TCCGGA [AT]^CCGG[AT] 629 TCCGGT BsmFI 1069 GGGAC GGGAC(10/14) 567 GTCCC BstF5I 603 CATCC GGATG(2/0) 724 CATCC CfoI 4 GCGC GCG^C 1077 GCGC DpnII 498 GATC ^GATC 646 GATC DraII 112 GGGGCCC [GA]G^G[ACGT]CC[CT] 113 GGGCCCC DraIII 405 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1036 CACAACGTG EcoO109I 112 GGGGCCC [GA]G^G[ACGT]CC[CT] 113 GGGCCCC EcoRV 541 GATATC GAT^ATC 691 GATATC EcoT14I 420 CCTAGG C^C[AT][AT]GG 1065 CCTTGG FokI 603 CATCC GGATG(9/13) 724 CATCC HhaI 4 GCGC GCG^C 1077 GCGC Hin1I 400 GACGTC G[GA]^CG[CT]C 635 GGCGTC HinP1I 4 GCGC G^CGC 1077 GCGC MaeI 308 CTAG C^TAG 421 CTAG MboI 498 GATC ^GATC 646 GATC NdeII 498 GATC ^GATC 646 GATC PleI 626 GACTC GAGTC(4/5) 831 GACTC PstI 988 CTGCAG CTGCA^G 999 CTGCAG Sau3AI 498 GATC ^GATC 646 GATC SfaNI 295 GATGC GCATC(5/9) 619 GATGC SfcI 988 CTGCAG C^T[GA][CT]AG 999 CTGCAG StyI 420 CCTAGG C^C[AT][AT]GG 1065 CCTTGG Tsp509I 579 AATT ^AATT 937 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AfaI AluI BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI TseI Tsp45I