(Circular) (Six-base) MAPSORT of: pFN21AE3147.seq Check: 6191 from: 1 to: 5785 pFN21AE3147 5785 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2409 3595 278 Size: 53 83 186 936 873 1186 2468 Fragments arranged by size: 2468 1186 936 873 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 2675 3106 2675 Size: 431 5354 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3848 5232 5500 1632 Size: 2216 1384 268 1917 Fragments arranged by size: 2216 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 2072 4514 2072 Size: 2442 3343 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 3528 5100 3528 Size: 1572 4213 ApaI G_GGCC'C Cuts at: 2122 2122 Size: 5785 ApaLI G'TGCA_C Cuts at: 2656 3842 4998 2656 Size: 1186 1156 3443 Fragments arranged by size: 3443 1186 1156 ApoI r'AATT_y Cuts at: 1493 2941 3094 3320 3472 5581 1493 Size: 1448 153 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 2205 4339 160 Size: 872 1173 2134 1606 Fragments arranged by size: 2134 1606 1173 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3110 1870 Size: 73 1167 4545 Fragments arranged by size: 4545 1167 73 AvrII C'CTAG_G Cuts at: 2425 2425 Size: 5785 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 3106 4561 618 Size: 325 217 627 888 431 1455 1842 Fragments arranged by size: 1842 1455 888 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 2032 2051 2122 3104 1391 Size: 641 19 71 982 4072 Fragments arranged by size: 4072 982 641 71 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 2458 2840 928 Size: 971 222 337 382 3873 Fragments arranged by size: 3873 971 382 337 222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2078 2112 3976 4010 1903 Size: 34 141 34 1864 34 3678 Fragments arranged by size: 3678 1864 141 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 2502 1548 Size: 954 4831 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 4396 136 Size: 107 122 71 631 605 637 2087 1525 Fragments arranged by size: 2087 1525 637 631 605 122 107 71 BglII A'GATC_T Cuts at: 2650 5780 2650 Size: 3130 2655 BlpI GC'TnA_GC Cuts at: 3021 3202 3021 Size: 181 5604 Bme1580I G_kGCm'C Cuts at: 1790 2122 2660 3846 5002 1790 Size: 332 538 1186 1156 2573 Fragments arranged by size: 2573 1186 1156 538 332 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2737 2737 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2735 4445 1239 Size: 760 736 1710 2579 Fragments arranged by size: 2579 1710 760 736 Bpu10I CC'TnA_GC Cuts at: 2904 2904 Size: 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2794 3910 4775 5073 2794 Size: 1116 865 298 3506 Fragments arranged by size: 3506 1116 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4448 882 Size: 617 2949 2219 Fragments arranged by size: 2949 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 3576 493 Size: 711 1560 812 2702 Fragments arranged by size: 2702 1560 812 711 BsaBI GATnn'nnATC Cuts at: 2550 5779 2550 Size: 3229 2556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2406 2641 3592 3974 Size: 53 83 186 936 873 235 951 382 Cuts at: 3974 4609 275 Size: 635 1451 Fragments arranged by size: 1451 951 936 873 635 382 235 186 83 53 BsaWI w'CCGG_w Cuts at: 2562 2634 4218 4616 4890 5037 5448 2562 Size: 72 1584 398 274 147 411 2899 Fragments arranged by size: 2899 1584 411 398 274 147 72 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2799 1186 Size: 1613 4172 BseYI C'CCAG_C Cuts at: 2025 2683 2881 2980 4988 5484 2025 Size: 658 198 99 2008 496 2326 Fragments arranged by size: 2326 2008 658 496 198 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3023 1163 Size: 573 1287 3925 Fragments arranged by size: 3925 1287 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2036 3235 3996 5024 1540 Size: 275 186 35 1199 761 1028 2301 Fragments arranged by size: 2301 1199 1028 761 275 186 35 BsiHKAI G_wGCw'C Cuts at: 2660 3846 3931 5002 2660 Size: 1186 85 1071 3443 Fragments arranged by size: 3443 1186 1071 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2051 2122 2660 3104 3846 3931 Size: 399 242 19 71 538 444 742 85 Cuts at: 3931 5002 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 538 444 399 242 85 71 19 BspEI T'CCGG_A Cuts at: 2562 5448 2562 Size: 2886 2899 BspHI T'CATG_A Cuts at: 2271 3674 5404 2271 Size: 1403 1730 2652 Fragments arranged by size: 2652 1730 1403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2811 3140 844 Size: 1967 329 3489 Fragments arranged by size: 3489 1967 329 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 2259 96 Size: 2163 3622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 2185 1184 Size: 1001 4784 BstXI CCAn_nnnn'nTGG Cuts at: 2461 2461 Size: 5785 BstYI r'GATC_y Cuts at: 1972 2650 3115 3482 3867 3884 5336 5422 5434 Size: 678 465 367 385 17 1452 86 12 Cuts at: 5434 5780 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 678 465 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2932 513 Size: 552 939 928 3366 Fragments arranged by size: 3366 939 928 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 3301 4095 4115 3301 Size: 794 20 4971 Fragments arranged by size: 4971 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DraIII CAC_nnn'GTG Cuts at: 2415 3046 2415 Size: 631 5154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2890 3232 4121 Size: 54 1425 50 275 18 1060 342 889 Cuts at: 4121 5750 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1060 889 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3715 1353 Size: 2362 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2387 4372 4746 4892 387 Size: 1064 138 798 1985 374 146 1280 Fragments arranged by size: 1985 1280 1064 798 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2735 3848 4445 5232 5500 1239 Size: 393 367 736 1113 597 787 268 1524 Fragments arranged by size: 1524 1113 787 736 597 393 367 268 EcoO109I rG'GnC_Cy Cuts at: 2118 2119 2118 Size: 1 5784 EcoRI G'AATT_C Cuts at: 2941 3094 3472 2941 Size: 153 378 5254 Fragments arranged by size: 5254 378 153 EcoRV GAT'ATC Cuts at: 2548 2698 2548 Size: 150 5635 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2012 4932 1678 Size: 334 2920 2531 Fragments arranged by size: 2920 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 4426 4458 4500 Size: 32 135 32 486 32 2336 32 42 Cuts at: 4500 4532 1373 Size: 32 2626 Fragments arranged by size: 2626 2336 486 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3129 3375 669 Size: 988 281 1191 246 3079 Fragments arranged by size: 3079 1191 988 281 246 HindIII A'AGCT_T Cuts at: 748 2135 748 Size: 1387 4398 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 2679 3110 2679 Size: 431 5354 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4899 5083 1305 Size: 3594 184 2007 Fragments arranged by size: 3594 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2892 10 Size: 54 1425 343 1060 2903 Fragments arranged by size: 2903 1425 1060 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2955 3743 4102 4261 518 Size: 648 1789 788 359 159 2042 Fragments arranged by size: 2042 1789 788 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2896 3201 3878 5026 5271 1606 Size: 1290 305 677 1148 245 2120 Fragments arranged by size: 2120 1290 1148 677 305 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 4159 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PmlI CAC'GTG Cuts at: 2764 2764 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PspOMI G'GGCC_C Cuts at: 2118 2118 Size: 5785 PstI C_TGCA'G Cuts at: 830 2997 3008 3137 830 Size: 2167 11 129 3478 Fragments arranged by size: 3478 2167 129 11 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2896 2896 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SexAI A'CCwGG_T Cuts at: 2460 2460 Size: 5785 SfcI C'TryA_G Cuts at: 826 1045 2993 3004 3133 4268 4949 5140 826 Size: 219 1948 11 129 1135 681 191 1471 Fragments arranged by size: 1948 1471 1135 681 219 191 129 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2773 3889 4790 5052 820 Size: 197 926 830 1116 901 262 1553 Fragments arranged by size: 1553 1116 926 901 830 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 2425 3070 3224 513 Size: 552 939 421 645 154 3074 Fragments arranged by size: 3074 939 645 552 421 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2022 2115 2592 2697 3812 3829 3982 Size: 1255 442 93 477 105 1115 17 153 Cuts at: 3982 4167 4612 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1115 477 445 442 185 153 105 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 4031 5743 Size: 274 80 33 51 1443 282 1772 1712 Cuts at: 5743 96 Size: 138 Fragments arranged by size: 1772 1712 1443 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2407 3593 276 Size: 53 83 186 936 873 1186 2468 Fragments arranged by size: 2468 1186 936 873 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI