Restriction map for
pFN21AE1742
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 472 CGCG CG^CG
AfaI 57 GTAC GT^AC
Aor51HI 359 AGCGCT AGC^GCT
ApaI 198 GGGCCC GGGCC^C
Asp700I 549 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 29 TGGCCA TGG^CCA
BamHI 573 GGATCC G^GATCC
BanI 584 GGCACC G^G[CT][GA]CC
BclI 320 TGATCA T^GATCA
BpmI 634 CTGGAG CTGGAG(16/14)
BsaI 492 GAGACC GGTCTC(1/5)
BsrDI 330 CATTGC GCAATG(2/0)
BsrI 27 ACTGG ACTGG(1/-1)
BstEII 279 GGTGACC G^GT[ACGT]ACC
BstPI 279 GGTGACC G^GT[ACGT]ACC
BstUI 472 CGCG CG^CG
BstXI 682 CCACTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 573 GGATCC [GA]^GATC[CT]
DraIII 391 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 29 TGGCCA [CT]^GGCC[GA]
EarI 172 CTCTTC CTCTTC(1/4)
Eco47III 359 AGCGCT AGC^GCT
EcoO65I 279 GGTGACC G^GT[ACGT]ACC
FbaI 320 TGATCA T^GATCA
HaeII 359 AGCGCT [GA]GCGC^[CT]
HinfI 465 GATTC G^A[ACGT]TC
Ksp632I 172 CTCTTC CTCTTC(1/4)
MflI 573 GGATCC [GA]^GATC[CT]
MluNI 29 TGGCCA TGG^CCA
MscI 29 TGGCCA TGG^CCA
MspA1I 530 CAGCTG C[AC]G^C[GT]G
MvnI 472 CGCG CG^CG
PvuII 530 CAGCTG CAG^CTG
RsaI 57 GTAC GT^AC
SacI 169 GAGCTC GAGCT^C
SapI 171 GCTCTTC GCTCTTC(1/4)
SbfI 183 CCTGCAGG CCTGCA^GG
SexAI 392 ACCAGGT A^CC[AT]GGT
Sse8387I 183 CCTGCAGG CCTGCA^GG
TaqI 541 TCGA T^CGA
TfiI 465 GATTC G^A[AT]TC
Tsp45I 280 GTGAC ^GT[GC]AC
Tsp509I 550 AATT ^AATT
TthHB8I 541 TCGA T^CGA
XcmI 393 CCAGGTGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 573 GGATCC [GA]^GATC[CT]
XmnI 549 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 574 GATCC GGATC(4/5)
573 GGATC
AspHI 169 GAGCTC G[AT]GC[AT]^C
441 GTGCTC
BanII 169 GAGCTC G[GA]GC[CT]^C
198 GGGCCC
BsiHKAI 169 GAGCTC G[AT]GC[AT]^C
441 GTGCTC
BsmAI 24 GAGAC GTCTC(1/5)
492 GAGAC
BsrBI 460 GAGCGG CCGCTC(-3/-3)
637 GAGCGG
CfoI 360 GCGC GCG^C
702 GCGC
DpnII 321 GATC ^GATC
574 GATC
HhaI 360 GCGC GCG^C
702 GCGC
HinP1I 360 GCGC G^CGC
702 GCGC
HphI 120 GGTGA GGTGA(8/7)
279 GGTGA
MaeIII 179 GTAAC ^GT[ACGT]AC
280 GTGAC
MboI 321 GATC ^GATC
574 GATC
MboII 450 GAAGA GAAGA(8/7)
173 TCTTC
NdeII 321 GATC ^GATC
574 GATC
NspI 386 ACATGC [GA]CATG^[CT]
522 GCATGT
Sau3AI 321 GATC ^GATC
574 GATC
TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^
683 CACTG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BspMI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI MnlI MslI MspI
MvaI MwoI NciI NlaIII NlaIV
PstI Sau96I ScrFI SfcI TseI