(Circular) (Six-base) MAPSORT of: pFN21AE1742.seq Check: 4962 from: 1 to: 5437 pFN21AE1742 5437 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2361 2361 Size: 5437 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 1937 Size: 843 4594 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2164 2684 3500 4884 5152 1632 Size: 532 520 816 1384 268 1917 Fragments arranged by size: 1917 1384 816 532 520 268 AfeI AGC'GCT Cuts at: 2366 2366 Size: 5437 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 2275 2438 1166 Size: 1109 163 4165 Fragments arranged by size: 4165 1109 163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2214 2456 1184 Size: 1030 242 4165 Fragments arranged by size: 4165 1030 242 AlwNI CAG_nnn'CTG Cuts at: 3180 4752 3180 Size: 1572 3865 ApaI G_GGCC'C Cuts at: 2207 2207 Size: 5437 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2746 2972 3124 5233 1493 Size: 1253 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2762 1870 Size: 73 819 4545 Fragments arranged by size: 4545 819 73 BamHI G'GATC_C Cuts at: 2578 2767 3134 2578 Size: 189 367 4881 Fragments arranged by size: 4881 367 189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2589 2758 4213 618 Size: 325 217 627 802 169 1455 1842 Fragments arranged by size: 1842 1455 802 627 325 217 169 BanII G_rGCy'C Cuts at: 1391 2178 2207 2756 1391 Size: 787 29 549 4072 Fragments arranged by size: 4072 787 549 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3628 3662 1903 Size: 34 1691 34 3678 Fragments arranged by size: 3678 1691 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4539 3329 Size: 1210 4227 BclI T'GATC_A Cuts at: 1548 2325 1548 Size: 777 4660 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4048 136 Size: 107 122 71 631 605 2376 1525 Fragments arranged by size: 2376 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5432 5432 Size: 5437 BlpI GC'TnA_GC Cuts at: 2854 2854 Size: 5437 Bme1580I G_kGCm'C Cuts at: 1790 2207 3498 4654 1790 Size: 417 1291 1156 2573 Fragments arranged by size: 2573 1291 1156 417 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 4126 442 Size: 416 53 83 399 24 1751 958 1753 Fragments arranged by size: 1753 1751 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2660 4097 1239 Size: 1421 1437 2579 Fragments arranged by size: 2579 1437 1421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2491 4100 882 Size: 617 992 1609 2219 Fragments arranged by size: 2219 1609 992 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3244 3626 4261 275 Size: 53 83 186 936 1711 382 635 1451 Fragments arranged by size: 1711 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5212 Fragments arranged by size: 5212 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5437 BseYI C'CCAG_C Cuts at: 4640 5136 4640 Size: 496 4941 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2299 2416 2506 1163 Size: 573 563 117 90 4094 Fragments arranged by size: 4094 573 563 117 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2887 3648 4676 1540 Size: 275 186 886 761 1028 2301 Fragments arranged by size: 2301 1028 886 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2178 2450 3498 3583 4654 2178 Size: 272 1048 85 1071 2961 Fragments arranged by size: 2961 1071 1048 272 85 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2207 2450 2756 3498 3583 4654 Size: 399 388 29 243 306 742 85 1071 Cuts at: 4654 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 388 306 243 85 29 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2196 2361 2792 844 Size: 1229 123 165 431 3489 Fragments arranged by size: 3489 1229 431 165 123 BsrBI CCG'CTC Cuts at: 1394 1823 2467 2644 3324 1394 Size: 429 644 177 680 3507 Fragments arranged by size: 3507 680 644 429 177 BsrDI GCAATG_nn' Cuts at: 65 1248 2334 3932 4106 65 Size: 1183 1086 1598 174 1396 Fragments arranged by size: 1598 1396 1183 1086 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4081 4268 1700 Size: 110 2271 187 2869 Fragments arranged by size: 2869 2271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 2284 1184 Size: 1100 4337 BstXI CCAn_nnnn'nTGG Cuts at: 2694 2694 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2578 2767 3134 3519 3536 4988 5074 5086 Size: 606 189 367 385 17 1452 86 12 Cuts at: 5086 5432 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 606 385 367 346 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3747 3767 2953 Size: 794 20 4623 Fragments arranged by size: 4623 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DraIII CAC_nnn'GTG Cuts at: 2401 2401 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2884 3773 Size: 54 1425 50 275 18 204 850 889 Cuts at: 3773 5402 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 850 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2884 1537 Size: 275 1072 4090 Fragments arranged by size: 4090 1072 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 3367 1353 Size: 830 1184 3423 Fragments arranged by size: 3423 1184 830 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2176 2176 Size: 5437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2164 2660 2684 3500 4097 4884 5152 Size: 393 532 496 24 816 597 787 268 Cuts at: 5152 1239 Size: 1524 Fragments arranged by size: 1524 816 787 597 532 496 393 268 24 EcoO109I rG'GnC_Cy Cuts at: 2204 2635 2683 2204 Size: 431 48 4958 Fragments arranged by size: 4958 431 48 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 2368 4584 1678 Size: 690 2216 2531 Fragments arranged by size: 2531 2216 690 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4078 4110 4152 4184 1373 Size: 32 135 32 2506 32 42 32 2626 Fragments arranged by size: 2626 2506 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2781 3027 669 Size: 988 281 843 246 3079 Fragments arranged by size: 3079 988 843 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4551 4735 1305 Size: 3246 184 2007 Fragments arranged by size: 3246 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 10 Size: 54 1425 343 204 3411 Fragments arranged by size: 3411 1425 343 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 2275 2438 2718 3395 3754 3913 518 Size: 648 1109 163 280 677 359 159 2042 Fragments arranged by size: 2042 1109 677 648 359 280 163 159 MspA1I CmG'CkG Cuts at: 1606 2537 2853 3530 4678 4923 1606 Size: 931 316 677 1148 245 2120 Fragments arranged by size: 2120 1148 931 677 316 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2395 2531 2795 4340 1132 Size: 97 1166 136 264 1545 2229 Fragments arranged by size: 2229 1545 1166 264 136 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5437 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PfoI T'CCnGG_A Cuts at: 2210 2574 2210 Size: 364 5073 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PspOMI G'GGCC_C Cuts at: 2203 2203 Size: 5437 PstI C_TGCA'G Cuts at: 830 2193 2358 2673 2789 830 Size: 1363 165 315 116 3478 Fragments arranged by size: 3478 1363 315 165 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 PvuII CAG'CTG Cuts at: 2537 2537 Size: 5437 SacI G_AGCT'C Cuts at: 2178 2178 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SapI GCTCTTCn'nnn_ Cuts at: 2183 2183 Size: 5437 SbfI CC_TGCA'GG Cuts at: 2193 2789 2193 Size: 596 4841 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SexAI A'CCwGG_T Cuts at: 2397 2397 Size: 5437 SfcI C'TryA_G Cuts at: 826 1045 2189 2354 2669 2785 3920 4601 4792 Size: 219 1144 165 315 116 1135 681 191 Cuts at: 4792 826 Size: 1471 Fragments arranged by size: 1471 1144 1135 681 315 219 191 165 116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2764 1872 Size: 892 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3541 4442 4704 820 Size: 197 926 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5437 StyI C'CwwG_G Cuts at: 513 1065 2004 2876 513 Size: 552 939 872 3074 Fragments arranged by size: 3074 939 872 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2265 2595 3464 3481 3634 3819 4264 Size: 1255 685 330 869 17 153 185 445 Cuts at: 4264 325 Size: 1498 Fragments arranged by size: 1498 1255 869 685 445 330 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2715 2747 5397 5429 2715 Size: 32 2650 32 2723 Fragments arranged by size: 2723 2650 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2405 2405 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2762 1870 Size: 892 4545 XmnI GAAnn'nnTTC Cuts at: 2558 3566 2558 Size: 1008 4429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI