Restriction map for
pFN21AE1742
(Linear) (Six-base) MAPSORT of: KIEE1742.seq Check: 4589 from: 1 to: 732
KIEE1742.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 356 732
Size: 356 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 679 732
Size: 159 520 53
Fragments arranged by size:
520 159 53
AfeI AGC'GCT
Cuts at: 0 361 732
Size: 361 371
AleI CACnn'nnGTG
Cuts at: 0 270 433 732
Size: 270 163 299
Fragments arranged by size:
299 270 163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 209 451 732
Size: 209 242 281
Fragments arranged by size:
281 242 209
ApaI G_GGCC'C
Cuts at: 0 202 732
Size: 202 530
BamHI G'GATC_C
Cuts at: 0 573 732
Size: 573 159
BanI G'GyrC_C
Cuts at: 0 584 732
Size: 584 148
BanII G_rGCy'C
Cuts at: 0 173 202 732
Size: 173 29 530
Fragments arranged by size:
530 173 29
BclI T'GATC_A
Cuts at: 0 320 732
Size: 320 412
Bme1580I G_kGCm'C
Cuts at: 0 202 732
Size: 202 530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 732
Size: 655 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 732
Size: 486 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 411 501 732
Size: 294 117 90 231
Fragments arranged by size:
294 231 117 90
BsiHKAI G_wGCw'C
Cuts at: 0 173 445 732
Size: 173 272 287
Fragments arranged by size:
287 272 173
Bsp1286I G_dGCh'C
Cuts at: 0 173 202 445 732
Size: 173 29 243 287
Fragments arranged by size:
287 243 173 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 191 356 732
Size: 68 123 165 376
Fragments arranged by size:
376 165 123 68
BsrBI CCG'CTC
Cuts at: 0 462 639 732
Size: 462 177 93
Fragments arranged by size:
462 177 93
BsrDI GCAATG_nn'
Cuts at: 0 329 732
Size: 329 403
BstEII G'GTnAC_C
Cuts at: 0 279 732
Size: 279 453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 689 732
Size: 689 43
BstYI r'GATC_y
Cuts at: 0 573 732
Size: 573 159
DraIII CAC_nnn'GTG
Cuts at: 0 396 732
Size: 396 336
EaeI y'GGCC_r
Cuts at: 0 29 732
Size: 29 703
EarI CTCTTCn'nnn_
Cuts at: 0 178 732
Size: 178 554
EcoICRI GAG'CTC
Cuts at: 0 171 732
Size: 171 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 655 679 732
Size: 159 496 24 53
Fragments arranged by size:
496 159 53 24
EcoO109I rG'GnC_Cy
Cuts at: 0 199 630 678 732
Size: 199 431 48 54
Fragments arranged by size:
431 199 54 48
HaeII r_GCGC'y
Cuts at: 0 363 732
Size: 363 369
MscI TGG'CCA
Cuts at: 0 31 732
Size: 31 701
MslI CAynn'nnrTG
Cuts at: 0 270 433 713 732
Size: 270 163 280 19
Fragments arranged by size:
280 270 163 19
MspA1I CmG'CkG
Cuts at: 0 532 732
Size: 532 200
NspI r_CATG'y
Cuts at: 0 390 526 732
Size: 390 136 206
Fragments arranged by size:
390 206 136
PfoI T'CCnGG_A
Cuts at: 0 205 569 732
Size: 205 364 163
Fragments arranged by size:
364 205 163
PspOMI G'GGCC_C
Cuts at: 0 198 732
Size: 198 534
PstI C_TGCA'G
Cuts at: 0 188 353 668 732
Size: 188 165 315 64
Fragments arranged by size:
315 188 165 64
PvuII CAG'CTG
Cuts at: 0 532 732
Size: 532 200
SacI G_AGCT'C
Cuts at: 0 173 732
Size: 173 559
SapI GCTCTTCn'nnn_
Cuts at: 0 178 732
Size: 178 554
SbfI CC_TGCA'GG
Cuts at: 0 188 732
Size: 188 544
SexAI A'CCwGG_T
Cuts at: 0 392 732
Size: 392 340
SfcI C'TryA_G
Cuts at: 0 184 349 664 732
Size: 184 165 315 68
Fragments arranged by size:
315 184 165 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 260 590 732
Size: 260 330 142
Fragments arranged by size:
330 260 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 710 732
Size: 710 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 400 732
Size: 400 332
XmnI GAAnn'nnTTC
Cuts at: 0 553 732
Size: 553 179
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI ApaI BamHI BanI
BanII BclI Bme1580I BpmI BsaI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BstEII BstXI BstYI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII MscI MslI MspA1I
NspI PfoI PspOMI PstI PvuII SacI SapI SbfI
SexAI SfcI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XhoI XmaI ZraI