################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AocI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbrPI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BsrI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflIII 584 ACACGT A^C[GA][CT]GT AhdI 709 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1037 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 538 AGCGCT AGC^GCT ApaI 137 GGGCCC GGGCC^C AspEI 709 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1465 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1068 GGACC G^G[AT]CC BalI 1169 TGGCCA TGG^CCA BanI 862 GGCACC G^G[CT][GA]CC BglI 1496 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 1252 GAGACC GGTCTC(1/5) BsmFI 637 GGGAC GGGAC(10/14) BsrBI 109 GAGCGG CCGCTC(-3/-3) BsrDI 644 GCAATG GCAATG(2/0) BsrFI 42 GCCGGT [GA]^CCGG[CT] BstAPI 1036 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 721 TTCGAA TT^CGAA BstXI 1160 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 42 GCCGGT [GA]^CCGG[CT] DraII 137 GGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 270 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 610 CGGCCG C^GGCCG Eam1105I 709 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 610 CGGCCG C^GGCCG Eco47III 538 AGCGCT AGC^GCT Eco52I 610 CGGCCG C^GGCCG EcoO109I 137 GGGCCCT [GA]G^G[ACGT]CC[CT] HaeII 538 AGCGCT [GA]GCGC^[CT] KspI 613 CCGCGG CCGC^GG MluNI 1169 TGGCCA TGG^CCA MscI 1169 TGGCCA TGG^CCA MseI 1382 TTAA T^TAA NotI 609 GCGGCCGC GC^GGCCGC NspV 721 TTCGAA TT^CGAA PflFI 1465 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 1354 GAGCTC GAGCT^C SacII 613 CCGCGG CCGC^GG SexAI 953 ACCTGGT A^CC[AT]GGT SfuI 721 TTCGAA TT^CGAA Tru9I 1382 TTAA T^TAA Tth111I 1465 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 777 CCAGCTGGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 714 GACGTC GACGT^C 1468 GACGTC AccII 614 CGCG CG^CG 947 CGCG Alw44I 180 GTGCAC G^TGCAC 982 GTGCAC ApaLI 180 GTGCAC G^TGCAC 982 GTGCAC AvaI 594 CTCGGG C^[CT]CG[GA]G 1556 CCCGAG BbvCI 1154 CCTCAGC CCTCAGC(-5/-2) 1455 CCTCAGC BcnI 522 CCGGG CC^[GC]GG 1514 CCGGG BsiEI 474 CGACCG CG[GA][CT]^CG 610 CGGCCG BsmBI 712 GAGACG CGTCTC(1/5) 943 GAGACG BsoBI 594 CTCGGG C^[CT]CG[GA]G 1556 CCCGAG BstUI 614 CGCG CG^CG 947 CGCG CfoI 539 GCGC GCG^C 948 GCGC EaeI 610 CGGCCG [CT]^GGCC[GA] 1169 TGGCCA EcoT14I 881 CCATGG C^C[AT][AT]GG 1130 CCATGG FauI 1109 CCCGC CCCGC(4/6) 126 GCGGG HgaI 945 GACGC GACGC(5/10) 35 GCGTC HhaI 539 GCGC GCG^C 948 GCGC HinP1I 539 GCGC G^CGC 948 GCGC HinfI 886 GACTC G^A[ACGT]TC 907 GACTC MvnI 614 CGCG CG^CG 947 CGCG NciI 522 CCGGG CC^[GC]GG 1514 CCGGG NcoI 881 CCATGG C^CATGG 1130 CCATGG PleI 886 GACTC GAGTC(4/5) 907 GACTC PstI 412 CTGCAG CTGCA^G 534 CTGCAG PvuII 400 CAGCTG CAG^CTG 778 CAGCTG ScaI 197 AGTACT AGT^ACT 1076 AGTACT StuI 56 AGGCCT AGG^CCT 1271 AGGCCT StyI 881 CCATGG C^C[AT][AT]GG 1130 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI BanII BbsI BbvI BcgI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HapII Hin1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XhoII