(Circular) (Six-base) MAPSORT of: pFN21AE5668.seq Check: 9909 from: 1 to: 6289 pFN21AE5668 6289 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2879 2879 Size: 6289 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2723 3477 4099 278 Size: 53 83 186 936 1187 754 622 2468 Fragments arranged by size: 2468 1187 936 754 622 186 83 53 AccI GT'mk_AC Cuts at: 1937 3632 1937 Size: 1695 4594 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2147 2568 2645 4352 5736 6004 1632 Size: 401 114 421 77 1707 1384 268 1917 Fragments arranged by size: 1917 1707 1384 421 401 268 114 77 AfeI AGC'GCT Cuts at: 2545 2545 Size: 6289 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 2589 5188 6165 1203 Size: 1386 2599 977 1327 Fragments arranged by size: 2599 1386 1327 977 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 2719 5018 2719 Size: 2299 3990 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6289 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2759 1184 Size: 1575 4714 AlwNI CAG_nnn'CTG Cuts at: 3047 4032 5604 3047 Size: 985 1572 3732 Fragments arranged by size: 3732 1572 985 ApaI G_GGCC'C Cuts at: 2146 2146 Size: 6289 ApaLI G'TGCA_C Cuts at: 2185 2987 4346 5502 2185 Size: 802 1359 1156 2972 Fragments arranged by size: 2972 1359 1156 802 ApoI r'AATT_y Cuts at: 1493 3598 3824 3976 6085 1493 Size: 2105 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 2599 3561 3614 1870 Size: 73 656 962 53 4545 Fragments arranged by size: 4545 962 656 73 53 BamHI G'GATC_C Cuts at: 3619 3986 3619 Size: 367 5922 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2867 3610 5065 618 Size: 325 217 627 1080 743 1455 1842 Fragments arranged by size: 1842 1455 1080 743 627 325 217 BanII G_rGCy'C Cuts at: 1391 2146 2162 3297 3363 3608 1391 Size: 755 16 1135 66 245 4072 Fragments arranged by size: 4072 1135 755 245 66 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2556 2557 2633 928 Size: 971 657 1 76 4584 Fragments arranged by size: 4584 971 657 76 1 BbvCI CC'TCA_GC Cuts at: 3160 3461 3160 Size: 301 5988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2228 2262 2480 2514 3396 3430 4480 Size: 34 291 34 218 34 882 34 1050 Cuts at: 4480 4514 1903 Size: 34 3678 Fragments arranged by size: 3678 1050 882 291 218 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4181 5391 4181 Size: 1210 5079 BclI T'GATC_A Cuts at: 1548 1548 Size: 6289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3507 4900 136 Size: 107 122 71 631 605 1835 1393 1525 Fragments arranged by size: 1835 1525 1393 631 605 122 107 71 BglII A'GATC_T Cuts at: 6284 6284 Size: 6289 BlpI GC'TnA_GC Cuts at: 3706 3706 Size: 6289 Bme1580I G_kGCm'C Cuts at: 1790 2137 2146 2189 2493 2870 2991 4350 5506 Size: 347 9 43 304 377 121 1359 1156 Cuts at: 5506 1790 Size: 2573 Fragments arranged by size: 2573 1359 1156 377 347 304 121 43 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6289 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4020 4978 442 Size: 416 53 83 399 24 2603 958 1753 Fragments arranged by size: 2603 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2429 3003 3377 4949 1239 Size: 1190 574 374 1572 2579 Fragments arranged by size: 2579 1572 1190 574 374 Bpu10I CC'TnA_GC Cuts at: 3160 3461 3160 Size: 301 5988 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4414 5279 5577 4414 Size: 865 298 5126 Fragments arranged by size: 5126 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3251 4952 882 Size: 617 1752 1701 2219 Fragments arranged by size: 2219 1752 1701 617 BsaAI yAC'GTr Cuts at: 493 1204 4080 493 Size: 711 2876 2702 Fragments arranged by size: 2876 2702 711 BsaBI GATnn'nnATC Cuts at: 6283 6283 Size: 6289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2720 3474 4096 Size: 53 83 186 936 507 680 754 622 Cuts at: 4096 4478 5113 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 754 680 635 622 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4722 5120 5394 5541 5952 4722 Size: 398 274 147 411 5059 Fragments arranged by size: 5059 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 1178 Size: 30 165 30 687 30 5347 Fragments arranged by size: 5347 687 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2039 2042 1186 Size: 850 3 3 5433 Fragments arranged by size: 5433 850 3 3 BseYI C'CCAG_C Cuts at: 2336 2486 2785 3149 5492 5988 2336 Size: 150 299 364 2343 496 2637 Fragments arranged by size: 2637 2343 496 364 299 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2219 2765 3060 1163 Size: 573 333 150 546 295 4392 Fragments arranged by size: 4392 573 546 333 295 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2482 2618 3739 4500 5528 1540 Size: 275 186 481 136 1121 761 1028 2301 Fragments arranged by size: 2301 1121 1028 761 481 275 186 136 BsiHKAI G_wGCw'C Cuts at: 2189 2991 3363 4350 4435 5506 2189 Size: 802 372 987 85 1071 2972 Fragments arranged by size: 2972 1071 987 802 372 85 BsmI GAATG_Cn' Cuts at: 3800 3893 3800 Size: 93 6196 BsmBI CGTCTCn'nnnn_ Cuts at: 2711 2942 2711 Size: 231 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2146 2162 2189 2493 2870 2991 Size: 399 347 9 16 27 304 377 121 Cuts at: 2991 3297 3363 3608 4350 4435 5506 1391 Size: 306 66 245 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 377 347 306 304 245 121 85 66 27 16 9 BspEI T'CCGG_A Cuts at: 5952 5952 Size: 6289 BspHI T'CATG_A Cuts at: 4178 5908 4178 Size: 1730 4559 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2700 2859 2879 3593 3644 844 Size: 1856 159 20 714 51 3489 Fragments arranged by size: 3489 1856 714 159 51 20 BsrBI CCG'CTC Cuts at: 1394 1823 2116 4176 1394 Size: 429 293 2060 3507 Fragments arranged by size: 3507 2060 429 293 BsrDI GCAATG_nn' Cuts at: 65 1248 2656 4784 4958 65 Size: 1183 1408 2128 174 1396 Fragments arranged by size: 2128 1408 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2047 4933 5120 1700 Size: 110 237 2886 187 2869 Fragments arranged by size: 2886 2869 237 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6289 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 4349 5361 4349 Size: 1012 5277 BstAPI GCAn_nnn'nTGC Cuts at: 794 3047 794 Size: 2253 4036 BstBI TT'CG_AA Cuts at: 2727 5093 2727 Size: 2366 3923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6289 BstXI CCAn_nnnn'nTGG Cuts at: 3172 3172 Size: 6289 BstYI r'GATC_y Cuts at: 1972 2748 3043 3144 3619 3986 4371 4388 5840 Size: 776 295 101 475 367 385 17 1452 Cuts at: 5840 5926 5938 6284 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 776 475 385 367 346 295 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2618 2886 3135 513 Size: 552 939 614 268 249 3667 Fragments arranged by size: 3667 939 614 552 268 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5550 Fragments arranged by size: 5550 683 56 BtsI GCAGTG_nn' Cuts at: 2081 2925 3445 3805 4599 4619 2081 Size: 844 520 360 794 20 3751 Fragments arranged by size: 3751 844 794 520 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6254 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DraIII CAC_nnn'GTG Cuts at: 2280 2280 Size: 6289 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5296 809 Size: 507 3980 1802 Fragments arranged by size: 3980 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2615 3174 3736 Size: 54 1425 50 275 18 785 559 562 Cuts at: 3736 4625 6254 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 785 562 559 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2615 3736 1537 Size: 275 803 1121 4090 Fragments arranged by size: 4090 1121 803 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4219 1353 Size: 2866 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4876 5250 5396 387 Size: 1064 138 3287 374 146 1280 Fragments arranged by size: 3287 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3361 3361 Size: 6289 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2147 2429 2568 2645 3003 3377 Size: 393 401 114 282 139 77 358 374 Cuts at: 3377 4352 4949 5736 6004 1239 Size: 975 597 787 268 1524 Fragments arranged by size: 1524 975 787 597 401 393 374 358 282 268 139 114 77 EcoO109I rG'GnC_Cy Cuts at: 2143 2143 Size: 6289 EcoRI G'AATT_C Cuts at: 3598 3976 3598 Size: 378 5911 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 6289 FspI TGC'GCA Cuts at: 4795 4795 Size: 6289 HaeII r_GCGC'y Cuts at: 1678 2547 5436 1678 Size: 869 2889 2531 Fragments arranged by size: 2889 2531 869 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 2705 2737 2740 Size: 32 135 32 533 32 568 32 3 Cuts at: 2740 2772 4930 4962 5004 5036 1373 Size: 32 2158 32 42 32 2626 Fragments arranged by size: 2626 2158 568 533 135 42 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 3633 3879 669 Size: 988 281 1695 246 3079 Fragments arranged by size: 3079 1695 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5403 5587 1305 Size: 4098 184 2007 Fragments arranged by size: 4098 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3176 10 Size: 54 1425 343 1344 3123 Fragments arranged by size: 3123 1425 1344 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 2741 3221 4247 4606 4765 518 Size: 648 1221 354 480 1026 359 159 2042 Fragments arranged by size: 2042 1221 1026 648 480 359 354 159 MspA1I CmG'CkG Cuts at: 1606 2407 2620 2785 2839 3705 4382 5530 5775 Size: 801 213 165 54 866 677 1148 245 Cuts at: 5775 1606 Size: 2120 Fragments arranged by size: 2120 1148 866 801 677 245 213 165 54 NcoI C'CATG_G Cuts at: 513 1065 2004 2886 3135 513 Size: 552 939 882 249 3667 Fragments arranged by size: 3667 939 882 552 249 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 2615 3736 2615 Size: 1121 5168 NspI r_CATG'y Cuts at: 1132 1229 2467 2509 2995 3647 5192 1132 Size: 97 1238 42 486 652 1545 2229 Fragments arranged by size: 2229 1545 1238 652 486 97 42 PasI CC'CwG_GG Cuts at: 1348 2862 1348 Size: 1514 4775 PciI A'CATG_T Cuts at: 5188 5188 Size: 6289 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6289 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4358 4390 5871 5903 4358 Size: 32 1481 32 4744 Fragments arranged by size: 4744 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6289 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PspOMI G'GGCC_C Cuts at: 2142 2142 Size: 6289 PstI C_TGCA'G Cuts at: 830 2421 2543 3641 830 Size: 1591 122 1098 3478 Fragments arranged by size: 3478 1591 1098 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 2407 2785 2407 Size: 378 5911 SacI G_AGCT'C Cuts at: 3363 3363 Size: 6289 SacII CC_GC'GG Cuts at: 2621 2621 Size: 6289 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 2204 3083 4537 6249 2204 Size: 879 1454 1712 2244 Fragments arranged by size: 2244 1712 1454 879 SexAI A'CCwGG_T Cuts at: 2958 2958 Size: 6289 SfcI C'TryA_G Cuts at: 826 1045 2417 2539 2917 3637 4772 5453 5644 Size: 219 1372 122 378 720 1135 681 191 Cuts at: 5644 826 Size: 1471 Fragments arranged by size: 1471 1372 1135 720 681 378 219 191 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 3616 1872 Size: 1744 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4393 5294 5556 820 Size: 197 926 2450 901 262 1553 Fragments arranged by size: 2450 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4080 493 Size: 3587 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 3647 1132 Size: 2515 3774 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 2063 3278 1517 Size: 546 1215 4528 Fragments arranged by size: 4528 1215 546 StyI C'CwwG_G Cuts at: 513 1065 2004 2886 3135 3728 513 Size: 552 939 882 249 593 3074 Fragments arranged by size: 3074 939 882 593 552 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2496 3290 4316 4333 4486 4671 Size: 1255 444 472 794 1026 17 153 185 Cuts at: 4671 5116 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1026 794 472 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 3014 3027 Size: 274 80 33 51 1443 225 812 13 Cuts at: 3027 3081 4535 6247 96 Size: 54 1454 1712 138 Fragments arranged by size: 1712 1454 1443 812 274 225 138 80 54 51 33 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 6249 6281 2090 Size: 32 4127 32 2098 Fragments arranged by size: 4127 2098 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3473 1316 Size: 2157 4132 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2789 2789 Size: 6289 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6289 XmaI C'CCGG_G Cuts at: 1870 3614 1870 Size: 1744 4545 XmnI GAAnn'nnTTC Cuts at: 4418 4418 Size: 6289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2721 3475 4097 276 Size: 53 83 186 936 1187 754 622 2468 Fragments arranged by size: 2468 1187 936 754 622 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI