(Linear) (Six-base) MAPSORT of: KIEE5668.seq Check: 1982 from: 1 to: 1584 KIEE5668.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 874 1584 Size: 874 710 AatII G_ACGT'C Cuts at: 0 718 1472 1584 Size: 718 754 112 Fragments arranged by size: 754 718 112 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 142 563 640 1584 Size: 28 114 421 77 944 Fragments arranged by size: 944 421 114 77 28 AfeI AGC'GCT Cuts at: 0 540 1584 Size: 540 1044 AflIII A'CryG_T Cuts at: 0 584 1584 Size: 584 1000 AhdI GACnn_n'nnGTC Cuts at: 0 714 1584 Size: 714 870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 754 1584 Size: 754 830 AlwNI CAG_nnn'CTG Cuts at: 0 1042 1584 Size: 1042 542 ApaI G_GGCC'C Cuts at: 0 141 1584 Size: 141 1443 ApaLI G'TGCA_C Cuts at: 0 180 982 1584 Size: 180 802 602 Fragments arranged by size: 802 602 180 AvaI C'yCGr_G Cuts at: 0 594 1556 1584 Size: 594 962 28 Fragments arranged by size: 962 594 28 BanI G'GyrC_C Cuts at: 0 862 1584 Size: 862 722 BanII G_rGCy'C Cuts at: 0 141 157 1292 1358 1584 Size: 141 16 1135 66 226 Fragments arranged by size: 1135 226 141 66 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 551 552 628 1584 Size: 551 1 76 956 Fragments arranged by size: 956 551 76 1 BbvCI CC'TCA_GC Cuts at: 0 1155 1456 1584 Size: 1155 301 128 Fragments arranged by size: 1155 301 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 223 257 475 509 1391 1425 1584 Size: 223 34 218 34 882 34 159 Fragments arranged by size: 882 223 218 159 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1502 1584 Size: 1502 82 Bme1580I G_kGCm'C Cuts at: 0 132 141 184 488 865 986 1584 Size: 132 9 43 304 377 121 598 Fragments arranged by size: 598 377 304 132 121 43 9 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 998 1372 1584 Size: 424 574 374 212 Fragments arranged by size: 574 424 374 212 Bpu10I CC'TnA_GC Cuts at: 0 1155 1456 1584 Size: 1155 301 128 Fragments arranged by size: 1155 301 128 BsaI GGTCTCn'nnnn_ Cuts at: 0 1246 1584 Size: 1246 338 BsaHI Gr'CG_yC Cuts at: 0 35 715 1469 1584 Size: 35 680 754 115 Fragments arranged by size: 754 680 115 35 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 85 115 1584 Size: 85 30 1469 Fragments arranged by size: 1469 85 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 31 34 37 1584 Size: 31 3 3 1547 Fragments arranged by size: 1547 31 3 3 BseYI C'CCAG_C Cuts at: 0 331 481 780 1144 1584 Size: 331 150 299 364 440 Fragments arranged by size: 440 364 331 299 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 214 760 1055 1584 Size: 64 150 546 295 529 Fragments arranged by size: 546 529 295 150 64 BsiEI CG_ry'CG Cuts at: 0 477 613 1584 Size: 477 136 971 Fragments arranged by size: 971 477 136 BsiHKAI G_wGCw'C Cuts at: 0 184 986 1358 1584 Size: 184 802 372 226 Fragments arranged by size: 802 372 226 184 BsmBI CGTCTCn'nnnn_ Cuts at: 0 706 937 1584 Size: 706 231 647 Fragments arranged by size: 706 647 231 Bsp1286I G_dGCh'C Cuts at: 0 132 141 157 184 488 865 986 1292 Size: 132 9 16 27 304 377 121 306 Cuts at: 1292 1358 1584 Size: 66 226 Fragments arranged by size: 377 306 304 226 132 121 66 27 16 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 695 854 874 1584 Size: 695 159 20 710 Fragments arranged by size: 710 695 159 20 BsrBI CCG'CTC Cuts at: 0 111 1584 Size: 111 1473 BsrDI GCAATG_nn' Cuts at: 0 651 1584 Size: 651 933 BsrFI r'CCGG_y Cuts at: 0 42 1584 Size: 42 1542 BstAPI GCAn_nnn'nTGC Cuts at: 0 1042 1584 Size: 1042 542 BstBI TT'CG_AA Cuts at: 0 722 1584 Size: 722 862 BstXI CCAn_nnnn'nTGG Cuts at: 0 1167 1584 Size: 1167 417 BstYI r'GATC_y Cuts at: 0 743 1038 1139 1584 Size: 743 295 101 445 Fragments arranged by size: 743 445 295 101 BtgI C'CryG_G Cuts at: 0 613 881 1130 1584 Size: 613 268 249 454 Fragments arranged by size: 613 454 268 249 BtsI GCAGTG_nn' Cuts at: 0 76 920 1440 1584 Size: 76 844 520 144 Fragments arranged by size: 844 520 144 76 DraIII CAC_nnn'GTG Cuts at: 0 275 1584 Size: 275 1309 EaeI y'GGCC_r Cuts at: 0 610 1169 1584 Size: 610 559 415 Fragments arranged by size: 610 559 415 EagI C'GGCC_G Cuts at: 0 610 1584 Size: 610 974 EcoICRI GAG'CTC Cuts at: 0 1356 1584 Size: 1356 228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 142 424 563 640 998 1372 1584 Size: 28 114 282 139 77 358 374 212 Fragments arranged by size: 374 358 282 212 139 114 77 28 EcoO109I rG'GnC_Cy Cuts at: 0 138 1584 Size: 138 1446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 576 1584 Size: 576 1008 HaeII r_GCGC'y Cuts at: 0 542 1584 Size: 542 1042 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 100 132 700 732 735 767 1584 Size: 100 32 568 32 3 32 817 Fragments arranged by size: 817 568 100 32 32 32 3 MscI TGG'CCA Cuts at: 0 1171 1584 Size: 1171 413 MslI CAynn'nnrTG Cuts at: 0 382 736 1216 1584 Size: 382 354 480 368 Fragments arranged by size: 480 382 368 354 MspA1I CmG'CkG Cuts at: 0 402 615 780 834 1584 Size: 402 213 165 54 750 Fragments arranged by size: 750 402 213 165 54 NcoI C'CATG_G Cuts at: 0 881 1130 1584 Size: 881 249 454 Fragments arranged by size: 881 454 249 NotI GC'GGCC_GC Cuts at: 0 610 1584 Size: 610 974 NspI r_CATG'y Cuts at: 0 462 504 990 1584 Size: 462 42 486 594 Fragments arranged by size: 594 486 462 42 PasI CC'CwG_GG Cuts at: 0 857 1584 Size: 857 727 PspOMI G'GGCC_C Cuts at: 0 137 1584 Size: 137 1447 PstI C_TGCA'G Cuts at: 0 416 538 1584 Size: 416 122 1046 Fragments arranged by size: 1046 416 122 PvuII CAG'CTG Cuts at: 0 402 780 1584 Size: 402 378 804 Fragments arranged by size: 804 402 378 SacI G_AGCT'C Cuts at: 0 1358 1584 Size: 1358 226 SacII CC_GC'GG Cuts at: 0 616 1584 Size: 616 968 ScaI AGT'ACT Cuts at: 0 199 1078 1584 Size: 199 879 506 Fragments arranged by size: 879 506 199 SexAI A'CCwGG_T Cuts at: 0 953 1584 Size: 953 631 SfcI C'TryA_G Cuts at: 0 412 534 912 1584 Size: 412 122 378 672 Fragments arranged by size: 672 412 378 122 StuI AGG'CCT Cuts at: 0 58 1273 1584 Size: 58 1215 311 Fragments arranged by size: 1215 311 58 StyI C'CwwG_G Cuts at: 0 881 1130 1584 Size: 881 249 454 Fragments arranged by size: 881 454 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 19 491 1285 1584 Size: 19 472 794 299 Fragments arranged by size: 794 472 299 19 TatI w'GTAC_w Cuts at: 0 197 1009 1022 1076 1584 Size: 197 812 13 54 508 Fragments arranged by size: 812 508 197 54 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 85 117 1584 Size: 85 32 1467 Fragments arranged by size: 1467 85 32 Tth111I GACn'n_nGTC Cuts at: 0 1468 1584 Size: 1468 116 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 784 1584 Size: 784 800 ZraI GAC'GTC Cuts at: 0 716 1470 1584 Size: 716 754 114 Fragments arranged by size: 754 716 114 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AhdI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I MscI MslI MspA1I NcoI NotI NspI PasI PspOMI PstI PvuII SacI SacII ScaI SexAI SfcI StuI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI