Restriction map for
pFN21AE3118
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsiEI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MslI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 62 CGCG CG^CG
AccIII 126 TCCGGA T^CCGGA
AflII 447 CTTAAG C^TTAAG
AseI 735 ATTAAT AT^TAAT
AsnI 735 ATTAAT AT^TAAT
AspHI 438 GAGCTC G[AT]GC[AT]^C
AvaI 187 CCCGAG C^[CT]CG[GA]G
AvaII 419 GGACC G^G[AT]CC
BbeI 79 GGCGCC GGCGC^C
BfrI 447 CTTAAG C^TTAAG
BglII 707 AGATCT A^GATCT
BsaWI 126 TCCGGA [AT]^CCGG[AT]
BseAI 126 TCCGGA T^CCGGA
BseRI 316 CTCCTC GAGGAG(10/8)
BsgI 703 GTGCAG GTGCAG(16/14)
BsiHKAI 438 GAGCTC G[AT]GC[AT]^C
BsoBI 187 CCCGAG C^[CT]CG[GA]G
BspEI 126 TCCGGA T^CCGGA
BspHI 407 TCATGA T^CATGA
BspMI 286 ACCTGC ACCTGC(4/8)
BstUI 62 CGCG CG^CG
Cac8I 351 GCGAGC GC[ACGT]^[ACGT]GC
CfoI 80 GCGC GCG^C
DraII 418 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 418 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 307 GAATTC G^AATTC
EcoT14I 528 CCATGG C^C[AT][AT]GG
HaeII 79 GGCGCC [GA]GCGC^[CT]
HgaI 624 GCGTC GACGC(5/10)
HhaI 80 GCGC GCG^C
HinP1I 80 GCGC G^CGC
KasI 79 GGCGCC G^GCGCC
MroI 126 TCCGGA T^CCGGA
MvnI 62 CGCG CG^CG
NarI 79 GGCGCC GG^CGCC
NcoI 528 CCATGG C^CATGG
NruI 61 TCGCGA TCG^CGA
PpuMI 418 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 735 ATTAAT AT^TAAT
PvuII 614 CAGCTG CAG^CTG
RcaI 407 TCATGA T^CATGA
SacI 438 GAGCTC GAGCT^C
SapI 780 GCTCTTC GCTCTTC(1/4)
SfoI 79 GGCGCC GGC^GCC
StyI 528 CCATGG C^C[AT][AT]GG
Tsp45I 683 GTGAC ^GT[GC]AC
TspRI 827 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 96 AAATTT [GA]^AATT[CT]
307 GAATTC
AcyI 79 GGCGCC G[GA]^CG[CT]C
623 GGCGTC
ApoI 96 AAATTT [GA]^AATT[CT]
307 GAATTC
BsaHI 79 GGCGCC G[GA]^CG[CT]C
623 GGCGTC
BsaI 40 GAGACC GGTCTC(1/5)
524 GAGACC
BsmBI 313 CGTCTC CGTCTC(1/5)
625 CGTCTC
BstXI 11 CCACGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
717 CCAGGATCATGG
DsaI 11 CCACGG C^C[GA][CT]GG
528 CCATGG
EarI 781 CTCTTC CTCTTC(1/4)
52 GAAGAG
Hin1I 79 GGCGCC G[GA]^CG[CT]C
623 GGCGTC
Ksp632I 781 CTCTTC CTCTTC(1/4)
52 GAAGAG
MaeII 215 ACGT A^CGT
462 ACGT
MboII 52 GAAGA GAAGA(8/7)
782 TCTTC
MspA1I 47 CAGCGG C[AC]G^C[GT]G
614 CAGCTG
NspI 488 GCATGT [GA]CATG^[CT]
686 ACATGC
SfaNI 295 GATGC GCATC(5/9)
379 GATGC
TaqI 165 TCGA T^CGA
212 TCGA
TthHB8I 165 TCGA T^CGA
212 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SmlI TfiI Tru9I
TseI Tsp509I XhoII