(Circular) (Six-base) MAPSORT of: pFN21AE3118.seq Check: 8486 from: 1 to: 5581 pFN21AE3118 5581 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3391 278 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2924 1937 Size: 987 4594 Acc65I G'GTAC_C Cuts at: 2902 2902 Size: 5581 AclI AA'CG_TT Cuts at: 1563 3708 4081 1563 Size: 2145 373 3063 Fragments arranged by size: 3063 2145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2773 3644 5028 5296 1632 Size: 1141 871 1384 268 1917 Fragments arranged by size: 1917 1384 1141 871 268 AflII C'TTAA_G Cuts at: 820 1017 2452 820 Size: 197 1435 3949 Fragments arranged by size: 3949 1435 197 AflIII A'CryG_T Cuts at: 1203 4480 5457 1203 Size: 3277 977 1327 Fragments arranged by size: 3277 1327 977 AgeI A'CCGG_T Cuts at: 4412 4412 Size: 5581 AhdI GACnn_n'nnGTC Cuts at: 4310 4310 Size: 5581 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5581 AlwNI CAG_nnn'CTG Cuts at: 3324 4896 3324 Size: 1572 4009 ApaLI G'TGCA_C Cuts at: 3638 4794 3638 Size: 1156 4425 ApoI r'AATT_y Cuts at: 1493 2101 2312 2890 3116 3268 5377 1493 Size: 608 211 578 226 152 2109 1697 Fragments arranged by size: 2109 1697 608 578 226 211 152 AseI AT'TA_AT Cuts at: 160 1032 2741 4135 160 Size: 872 1709 1394 1606 Fragments arranged by size: 1709 1606 1394 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 AvaI C'yCGr_G Cuts at: 1870 1943 2192 2906 1870 Size: 73 249 714 4545 Fragments arranged by size: 4545 714 249 73 BamHI G'GATC_C Cuts at: 2911 3278 2911 Size: 367 5214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2077 2084 2902 4357 Size: 325 217 627 224 66 7 818 1455 Cuts at: 4357 618 Size: 1842 Fragments arranged by size: 1842 1455 818 627 325 224 217 66 7 BanII G_rGCy'C Cuts at: 1391 2217 2447 2595 2900 1391 Size: 826 230 148 305 4072 Fragments arranged by size: 4072 826 305 230 148 BbeI G_GCGC'C Cuts at: 2088 2088 Size: 5581 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3772 3806 1903 Size: 34 1835 34 3678 Fragments arranged by size: 3678 1835 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3473 4683 3473 Size: 1210 4371 BclI T'GATC_A Cuts at: 1548 1548 Size: 5581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4192 136 Size: 107 122 71 631 605 2520 1525 Fragments arranged by size: 2520 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2712 5576 2712 Size: 2864 2717 BlpI GC'TnA_GC Cuts at: 2998 2998 Size: 5581 Bme1580I G_kGCm'C Cuts at: 1790 2016 3642 4798 1790 Size: 226 1626 1156 2573 Fragments arranged by size: 2573 1626 1156 226 BmgBI CAC'GTC Cuts at: 1318 2221 1318 Size: 903 4678 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3312 4270 442 Size: 416 53 83 399 24 1895 958 1753 Fragments arranged by size: 1895 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5581 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2268 2268 Size: 5581 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4241 1239 Size: 3002 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2290 2461 3706 4571 4869 2290 Size: 171 1245 865 298 3002 Fragments arranged by size: 3002 1245 865 298 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2039 2523 4244 882 Size: 617 540 484 1721 2219 Fragments arranged by size: 2219 1721 617 540 484 BsaAI yAC'GTr Cuts at: 493 1204 3372 493 Size: 711 2168 2702 Fragments arranged by size: 2702 2168 711 BsaBI GATnn'nnATC Cuts at: 5575 5575 Size: 5581 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2629 3388 3770 Size: 53 83 186 936 552 544 759 382 Cuts at: 3770 4405 275 Size: 635 1451 Fragments arranged by size: 1451 936 759 635 552 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2131 4014 4412 4686 4833 5244 2131 Size: 1883 398 274 147 411 2468 Fragments arranged by size: 2468 1883 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 2255 2285 2654 Size: 30 165 30 619 30 203 30 369 Cuts at: 2654 2684 1178 Size: 30 4075 Fragments arranged by size: 4075 619 369 203 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 1186 Size: 1126 4455 BseYI C'CCAG_C Cuts at: 2641 4784 5280 2641 Size: 2143 496 2942 Fragments arranged by size: 2942 2143 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2729 1163 Size: 573 993 4015 Fragments arranged by size: 4015 993 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3031 3792 4820 1540 Size: 275 186 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2447 3642 3727 4798 2447 Size: 1195 85 1071 3230 Fragments arranged by size: 3230 1195 1071 85 BsmI GAATG_Cn' Cuts at: 3092 3185 3092 Size: 93 5488 BsmBI CGTCTCn'nnnn_ Cuts at: 2324 2636 2324 Size: 312 5269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2217 2447 2595 2900 3642 3727 Size: 399 226 201 230 148 305 742 85 Cuts at: 3727 4798 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 305 230 226 201 148 85 BspEI T'CCGG_A Cuts at: 2131 5244 2131 Size: 3113 2468 BspHI T'CATG_A Cuts at: 2412 3470 5200 2412 Size: 1058 1730 2793 Fragments arranged by size: 2793 1730 1058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2300 2869 2936 844 Size: 1456 569 67 3489 Fragments arranged by size: 3489 1456 569 67 BsrBI CCG'CTC Cuts at: 1394 1823 3468 1394 Size: 429 1645 3507 Fragments arranged by size: 3507 1645 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4076 4250 65 Size: 1183 2828 174 1396 Fragments arranged by size: 2828 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4225 4412 1700 Size: 110 2415 187 2869 Fragments arranged by size: 2869 2415 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5581 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5581 BssSI C'ACGA_G Cuts at: 3641 4653 3641 Size: 1012 4569 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5581 BstBI TT'CG_AA Cuts at: 4385 4385 Size: 5581 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5581 BstXI CCAn_nnnn'nTGG Cuts at: 2023 2729 2023 Size: 706 4875 BstYI r'GATC_y Cuts at: 1972 2239 2338 2712 2911 3278 3663 3680 5132 Size: 267 99 374 199 367 385 17 1452 Cuts at: 5132 5218 5230 5576 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 374 367 346 267 199 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2533 513 Size: 552 939 12 517 3561 Fragments arranged by size: 3561 939 552 517 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4842 Fragments arranged by size: 4842 683 56 BtsI GCAGTG_nn' Cuts at: 3097 3891 3911 3097 Size: 794 20 4767 Fragments arranged by size: 4767 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5581 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5546 DraI TTT'AAA Cuts at: 2885 2885 Size: 5581 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4588 809 Size: 507 3272 1802 Fragments arranged by size: 3272 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546 Size: 54 1425 50 275 18 1198 889 1629 Cuts at: 5546 8 Size: 43 Fragments arranged by size: 1629 1425 1198 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3028 1537 Size: 275 1216 4090 Fragments arranged by size: 4090 1216 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2792 3511 1353 Size: 699 740 719 3423 Fragments arranged by size: 3423 740 719 699 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 4168 4542 4688 387 Size: 1064 138 546 2033 374 146 1280 Fragments arranged by size: 2033 1280 1064 546 374 146 138 EcoICRI GAG'CTC Cuts at: 2445 2445 Size: 5581 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2773 3644 4241 5028 5296 1239 Size: 393 1141 871 597 787 268 1524 Fragments arranged by size: 1524 1141 871 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2424 2424 Size: 5581 EcoRI G'AATT_C Cuts at: 2312 2890 3268 2312 Size: 578 378 4625 Fragments arranged by size: 4625 578 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 5581 FspI TGC'GCA Cuts at: 4087 4087 Size: 5581 HaeII r_GCGC'y Cuts at: 1678 2088 4728 1678 Size: 410 2640 2531 Fragments arranged by size: 2640 2531 410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2710 2742 4222 4254 4296 Size: 32 135 32 1138 32 1480 32 42 Cuts at: 4296 4328 1373 Size: 32 2626 Fragments arranged by size: 2626 1480 1138 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2925 3171 669 Size: 988 281 987 246 3079 Fragments arranged by size: 3079 988 987 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5581 HpaI GTT'AAC Cuts at: 3171 3171 Size: 5581 KasI G'GCGC_C Cuts at: 2084 2084 Size: 5581 KpnI G_GTAC'C Cuts at: 2906 2906 Size: 5581 MfeI C'AATT_G Cuts at: 3180 3180 Size: 5581 MluI A'CGCG_T Cuts at: 5457 5457 Size: 5581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4695 4879 1305 Size: 3390 184 2007 Fragments arranged by size: 3390 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3759 Fragments arranged by size: 3759 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3539 3898 4057 518 Size: 648 843 1530 359 159 2042 Fragments arranged by size: 2042 1530 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2621 2997 3674 4822 5067 1606 Size: 448 567 376 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 567 448 376 245 NarI GG'CG_CC Cuts at: 2085 2085 Size: 5581 NcoI C'CATG_G Cuts at: 513 1065 2004 2533 513 Size: 552 939 529 3561 Fragments arranged by size: 3561 939 552 529 NdeI CA'TA_TG Cuts at: 387 387 Size: 5581 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5581 NotI GC'GGCC_GC Cuts at: 3028 3028 Size: 5581 NruI TCG'CGA Cuts at: 2068 2068 Size: 5581 NspI r_CATG'y Cuts at: 1132 1229 2497 2695 2939 4484 1132 Size: 97 1268 198 244 1545 2229 Fragments arranged by size: 2229 1545 1268 244 198 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5581 PciI A'CATG_T Cuts at: 4480 4480 Size: 5581 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5581 PmeI GTTT'AAAC Cuts at: 2885 2885 Size: 5581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3650 3682 5163 5195 3650 Size: 32 1481 32 4036 Fragments arranged by size: 4036 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2424 2424 Size: 5581 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5581 PsiI TTA'TAA Cuts at: 3151 3151 Size: 5581 PstI C_TGCA'G Cuts at: 830 2933 830 Size: 2103 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5581 PvuII CAG'CTG Cuts at: 2621 2621 Size: 5581 SacI G_AGCT'C Cuts at: 2447 2447 Size: 5581 SalI G'TCGA_C Cuts at: 1936 2923 1936 Size: 987 4594 SapI GCTCTTCn'nnn_ Cuts at: 2792 2792 Size: 5581 SbfI CC_TGCA'GG Cuts at: 2933 2933 Size: 5581 ScaI AGT'ACT Cuts at: 3829 5541 3829 Size: 1712 3869 SfcI C'TryA_G Cuts at: 826 1045 2929 4064 4745 4936 826 Size: 219 1884 1135 681 191 1471 Fragments arranged by size: 1884 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2086 2086 Size: 5581 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5581 SmaI CCC'GGG Cuts at: 1872 2908 1872 Size: 1036 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2305 2440 2452 3685 4586 4848 Size: 197 926 362 135 12 1233 901 262 Cuts at: 4848 820 Size: 1553 Fragments arranged by size: 1553 1233 926 901 362 262 197 135 12 SnaBI TAC'GTA Cuts at: 493 3372 493 Size: 2879 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5581 SphI G_CATG'C Cuts at: 1132 2939 1132 Size: 1807 3774 SspI AAT'ATT Cuts at: 5 52 3505 5 Size: 47 3453 2081 Fragments arranged by size: 3453 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5581 StyI C'CwwG_G Cuts at: 513 1065 2004 2533 3020 513 Size: 552 939 529 487 3074 Fragments arranged by size: 3074 939 552 529 487 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3608 3625 3778 3963 4408 325 Size: 1255 2028 17 153 185 445 1498 Fragments arranged by size: 2028 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3827 5539 96 Size: 274 80 33 51 1443 1850 1712 138 Fragments arranged by size: 1850 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2255 2287 2654 2686 5541 5573 2255 Size: 32 367 32 2855 32 2263 Fragments arranged by size: 2855 2263 367 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5581 XbaI T'CTAG_A Cuts at: 2917 2917 Size: 5581 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5581 XmaI C'CCGG_G Cuts at: 1870 2906 1870 Size: 1036 4545 XmnI GAAnn'nnTTC Cuts at: 3710 3710 Size: 5581 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3389 276 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI