################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 925 GTCTAC GT^[AC][GT]AC AlwI 462 GGATC GGATC(4/5) AspHI 1063 GTGCTC G[AT]GC[AT]^C AvaII 237 GGTCC G^G[AT]CC AviII 188 TGCGCA TGC^GCA BanI 704 GGCGCC G^G[CT][GA]CC BbeI 704 GGCGCC GGCGC^C BbvCI 468 GCTGAGG CCTCAGC(-5/-2) BfaI 175 CTAG C^TAG BseRI 7 GAGGAG GAGGAG(10/8) BsiHKAI 1063 GTGCTC G[AT]GC[AT]^C BsmI 677 GAATGC GAATGC(1/-1) BsrBI 829 GAGCGG CCGCTC(-3/-3) DpnII 463 GATC ^GATC FauI 43 GCGGG CCCGC(4/6) FspI 188 TGCGCA TGC^GCA KasI 704 GGCGCC G^GCGCC KspI 19 CCGCGG CCGC^GG MaeI 175 CTAG C^TAG MboI 463 GATC ^GATC MboII 848 TCTTC GAAGA(8/7) MslI 578 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 704 GGCGCC GG^CGCC NdeII 463 GATC ^GATC NotI 299 GCGGCCGC GC^GGCCGC NspI 778 ACATGC [GA]CATG^[CT] PflMI 600 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 19 CCGCGG CCGC^GG Sau3AI 463 GATC ^GATC SfoI 704 GGCGCC GGC^GCC SmaI 659 CCCGGG CCC^GGG StuI 177 AGGCCT AGG^CCT Tsp45I 202 GTGAC ^GT[GC]AC Tsp509I 871 AATT ^AATT Van91I 600 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1010 CCAGCCGCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 659 CCCGGG C^CCGGG XmaI 659 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 207 GGCGTC G[GA]^CG[CT]C 704 GGCGCC AlwNI 220 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 493 CAGGCACTG Aor51HI 269 AGCGCT AGC^GCT 1085 AGCGCT AspI 147 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 204 GACGGCGTC BsaHI 207 GGCGTC G[GA]^CG[CT]C 704 GGCGCC BsaI 959 GAGACC GGTCTC(1/5) 46 GGTCTC BsgI 226 CTGCAC GTGCAG(16/14) 66 GTGCAG BsiEI 16 CGGCCG CG[GA][CT]^CG 300 CGGCCG BsrDI 1093 CATTGC GCAATG(2/0) 83 GCAATG BsrI 1106 ACTGG ACTGG(1/-1) 768 CCAGT BssSI 241 CACGAG CACGAG(-5/-1) 1076 CACGAG DraIII 135 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1057 CACAACGTG EagI 16 CGGCCG C^GGCCG 300 CGGCCG EclXI 16 CGGCCG C^GGCCG 300 CGGCCG Eco47III 269 AGCGCT AGC^GCT 1085 AGCGCT Eco52I 16 CGGCCG C^GGCCG 300 CGGCCG Hin1I 207 GGCGTC G[GA]^CG[CT]C 704 GGCGCC MaeII 200 ACGT A^CGT 1061 ACGT PflFI 147 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 204 GACGGCGTC Tth111I 147 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 204 GACGGCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AluI AvaI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI PvuII Sau96I ScrFI SfcI SmlI TfiI TseI TspRI