(Linear) (Six-base) MAPSORT of: KIEE1868.seq Check: 1421 from: 1 to: 1167 KIEE1868.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AccI GT'mk_AC Cuts at: 0 926 1167 Size: 926 241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 202 748 1167 Size: 121 81 546 419 Fragments arranged by size: 546 419 121 81 AfeI AGC'GCT Cuts at: 0 271 1087 1167 Size: 271 816 80 Fragments arranged by size: 816 271 80 AlwNI CAG_nnn'CTG Cuts at: 0 225 498 1167 Size: 225 273 669 Fragments arranged by size: 669 273 225 AvaI C'yCGr_G Cuts at: 0 33 503 629 659 1167 Size: 33 470 126 30 508 Fragments arranged by size: 508 470 126 33 30 BanI G'GyrC_C Cuts at: 0 704 1167 Size: 704 463 BanII G_rGCy'C Cuts at: 0 14 78 976 1167 Size: 14 64 898 191 Fragments arranged by size: 898 191 64 14 BbeI G_GCGC'C Cuts at: 0 708 1167 Size: 708 459 BbvCI CC'TCA_GC Cuts at: 0 469 1167 Size: 469 698 Bme1580I G_kGCm'C Cuts at: 0 1058 1167 Size: 1058 109 BmgBI CAC'GTC Cuts at: 0 201 1167 Size: 201 966 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 642 1167 Size: 642 525 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 804 896 1167 Size: 25 779 92 271 Fragments arranged by size: 779 271 92 25 Bpu10I CC'TnA_GC Cuts at: 0 160 469 1167 Size: 160 309 698 Fragments arranged by size: 698 309 160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 313 534 1167 Size: 310 3 221 633 Fragments arranged by size: 633 310 221 3 BsaI GGTCTCn'nnnn_ Cuts at: 0 52 953 1167 Size: 52 901 214 Fragments arranged by size: 901 214 52 BsaHI Gr'CG_yC Cuts at: 0 208 705 1167 Size: 208 497 462 Fragments arranged by size: 497 462 208 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 908 938 1167 Size: 908 30 229 Fragments arranged by size: 908 229 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 1167 Size: 22 1145 BseYI C'CCAG_C Cuts at: 0 376 532 556 834 1167 Size: 376 156 24 278 333 Fragments arranged by size: 376 333 278 156 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 87 211 1167 Size: 87 124 956 Fragments arranged by size: 956 124 87 BsiEI CG_ry'CG Cuts at: 0 19 303 1167 Size: 19 284 864 Fragments arranged by size: 864 284 19 BsiHKAI G_wGCw'C Cuts at: 0 1067 1167 Size: 1067 100 BsmI GAATG_Cn' Cuts at: 0 683 1167 Size: 683 484 Bsp1286I G_dGCh'C Cuts at: 0 14 78 976 1058 1067 1167 Size: 14 64 898 82 9 100 Fragments arranged by size: 898 100 82 64 14 9 BsrBI CCG'CTC Cuts at: 0 831 1167 Size: 831 336 BsrDI GCAATG_nn' Cuts at: 0 90 1092 1167 Size: 90 1002 75 Fragments arranged by size: 1002 90 75 BssSI C'ACGA_G Cuts at: 0 241 1076 1167 Size: 241 835 91 Fragments arranged by size: 835 241 91 BstAPI GCAn_nnn'nTGC Cuts at: 0 258 1040 1062 1167 Size: 258 782 22 105 Fragments arranged by size: 782 258 105 22 BtgI C'CryG_G Cuts at: 0 19 398 1050 1167 Size: 19 379 652 117 Fragments arranged by size: 652 379 117 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 216 1167 Size: 216 951 BtsI GCAGTG_nn' Cuts at: 0 456 815 1167 Size: 456 359 352 Fragments arranged by size: 456 359 352 DraIII CAC_nnn'GTG Cuts at: 0 140 1062 1167 Size: 140 922 105 Fragments arranged by size: 922 140 105 EaeI y'GGCC_r Cuts at: 0 16 128 300 1047 1167 Size: 16 112 172 747 120 Fragments arranged by size: 747 172 120 112 16 EagI C'GGCC_G Cuts at: 0 16 300 1167 Size: 16 284 867 Fragments arranged by size: 867 284 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 17 1167 Size: 17 1150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 121 202 748 804 896 1167 Size: 25 96 81 546 56 92 271 Fragments arranged by size: 546 271 96 92 81 56 25 FspI TGC'GCA Cuts at: 0 190 1167 Size: 190 977 HaeII r_GCGC'y Cuts at: 0 273 323 708 1089 1167 Size: 273 50 385 381 78 Fragments arranged by size: 385 381 273 78 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 908 940 1167 Size: 908 32 227 Fragments arranged by size: 908 227 32 KasI G'GCGC_C Cuts at: 0 704 1167 Size: 704 463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1001 1167 Size: 1001 166 MslI CAynn'nnrTG Cuts at: 0 582 1167 Size: 582 585 MspA1I CmG'CkG Cuts at: 0 21 123 258 489 764 1167 Size: 21 102 135 231 275 403 Fragments arranged by size: 403 275 231 135 102 21 NarI GG'CG_CC Cuts at: 0 705 1167 Size: 705 462 NotI GC'GGCC_GC Cuts at: 0 300 1167 Size: 300 867 NspI r_CATG'y Cuts at: 0 782 1167 Size: 782 385 PasI CC'CwG_GG Cuts at: 0 979 1167 Size: 979 188 PflMI CCAn_nnn'nTGG Cuts at: 0 606 1167 Size: 606 561 PstI C_TGCA'G Cuts at: 0 409 488 494 1167 Size: 409 79 6 673 Fragments arranged by size: 673 409 79 6 PvuII CAG'CTG Cuts at: 0 258 489 764 1167 Size: 258 231 275 403 Fragments arranged by size: 403 275 258 231 SacII CC_GC'GG Cuts at: 0 22 1167 Size: 22 1145 SfcI C'TryA_G Cuts at: 0 405 484 490 1167 Size: 405 79 6 677 Fragments arranged by size: 677 405 79 6 SfoI GGC'GCC Cuts at: 0 706 1167 Size: 706 461 SmaI CCC'GGG Cuts at: 0 661 1167 Size: 661 506 SmlI C'TyrA_G Cuts at: 0 289 328 549 1167 Size: 289 39 221 618 Fragments arranged by size: 618 289 221 39 StuI AGG'CCT Cuts at: 0 179 1167 Size: 179 988 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1140 1167 Size: 1140 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 435 467 1167 Size: 435 32 700 Fragments arranged by size: 700 435 32 Tth111I GACn'n_nGTC Cuts at: 0 150 207 1167 Size: 150 57 960 Fragments arranged by size: 960 150 57 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1017 1167 Size: 1017 150 XmaI C'CCGG_G Cuts at: 0 659 1167 Size: 659 508 Enzymes that do cut: AccI AcuI AfeI AlwNI AvaI BanI BanII BbeI BbvCI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BssSI BstAPI BtgI BtgZI BtsI DraIII EaeI EagI EciI Eco57MI FspI HaeII Hin4I KasI MmeI MslI MspA1I NarI NotI NspI PasI PflMI PstI PvuII SacII SfcI SfoI SmaI SmlI StuI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI XbaI XhoI XmnI ZraI