################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BpuAI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 88 GACGTC GACGT^C AcyI 88 GACGTC G[GA]^CG[CT]C AflIII 498 ACATGT A^C[GA][CT]GT Aor51HI 716 AGCGCT AGC^GCT Asp700I 496 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 519 GGTGCC G^G[CT][GA]CC BglII 409 AGATCT A^GATCT BlpI 142 GCTCAGC GC^T[ACGT]AGC Bpu1102I 142 GCTCAGC GC^T[ACGT]AGC BsaAI 16 TACGTG [CT]AC^GT[GA] BsaBI 633 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 88 GACGTC G[GA]^CG[CT]C BspLU11I 498 ACATGT A^CATGT BstXI 240 CCAGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 142 GCTCAGC GC^T[ACGT]AGC EarI 598 CTCTTC CTCTTC(1/4) Eco47III 716 AGCGCT AGC^GCT Hin1I 88 GACGTC G[GA]^CG[CT]C HincII 253 GTCAAC GT[CT]^[GA]AC HindII 253 GTCAAC GT[CT]^[GA]AC Ksp632I 598 CTCTTC CTCTTC(1/4) MamI 633 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 284 TTAA T^TAA MslI 576 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 498 ACATGT [GA]CATG^[CT] PshAI 195 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 790 CTGCAG CTGCA^G SacI 1224 GAGCTC GAGCT^C SfcI 790 CTGCAG C^T[GA][CT]AG SmlI 1183 CTTGAG C^T[CT][GA]AG TfiI 1005 GATTC G^A[AT]TC Tru9I 284 TTAA T^TAA XmnI 496 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BalI 779 TGGCCA TGG^CCA 839 TGGCCA BanII 243 GGGCTC G[GA]GC[CT]^C 1224 GAGCTC BsaWI 987 ACCGGA [AT]^CCGG[AT] 1409 ACCGGA BsgI 277 CTGCAC GTGCAG(16/14) 748 GTGCAG BsmAI 91 GTCTC GTCTC(1/5) 905 GTCTC BsmBI 90 CGTCTC CGTCTC(1/5) 904 CGTCTC BsrBI 729 CCGCTC CCGCTC(-3/-3) 1186 GAGCGG BsrDI 738 CATTGC GCAATG(2/0) 299 GCAATG BsrFI 1370 ACCGGC [GA]^CCGG[CT] 1427 ACCGGC Cfr10I 1370 ACCGGC [GA]^CCGG[CT] 1427 ACCGGC DraII 1110 GGGTCCT [GA]G^G[ACGT]CC[CT] 1301 AGGACCT DrdI 82 GACTCAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1103 GACTTCTGGGTC EaeI 779 TGGCCA [CT]^GGCC[GA] 839 TGGCCA EcoNI 96 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1272 CCTGGAGGAGG EcoO109I 1110 GGGTCCT [GA]G^G[ACGT]CC[CT] 1301 AGGACCT EcoT14I 1432 CCTTGG C^C[AT][AT]GG 1439 CCAAGG HaeII 365 AGCGCC [GA]GCGC^[CT] 716 AGCGCT MboII 599 TCTTC GAAGA(8/7) 1249 TCTTC MluNI 779 TGGCCA TGG^CCA 839 TGGCCA MscI 779 TGGCCA TGG^CCA 839 TGGCCA NdeI 509 CATATG CA^TATG 962 CATATG PflMI 363 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1087 CCAGCCCCTGG PpuMI 1110 GGGTCCT [GA]G^G[AT]CC[CT] 1301 AGGACCT StyI 1432 CCTTGG C^C[AT][AT]GG 1439 CCAAGG Van91I 363 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1087 CCAGCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmaI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI