(Linear) (Six-base) MAPSORT of: KIEE3873.seq Check: 3929 from: 1 to: 1452 KIEE3873.orf With 182 enzymes: SgfI * August 4, 2021 20:20 .. AatII G_ACGT'C Cuts at: 0 92 1452 Size: 92 1360 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1120 1452 Size: 1120 332 AfeI AGC'GCT Cuts at: 0 718 1452 Size: 718 734 AflIII A'CryG_T Cuts at: 0 498 1452 Size: 498 954 AlwNI CAG_nnn'CTG Cuts at: 0 209 369 1093 1149 1152 1305 1452 Size: 209 160 724 56 3 153 147 Fragments arranged by size: 724 209 160 153 147 56 3 AvaI C'yCGr_G Cuts at: 0 22 644 917 1128 1332 1452 Size: 22 622 273 211 204 120 Fragments arranged by size: 622 273 211 204 120 22 BanI G'GyrC_C Cuts at: 0 519 1452 Size: 519 933 BanII G_rGCy'C Cuts at: 0 247 1228 1452 Size: 247 981 224 Fragments arranged by size: 981 247 224 BglII A'GATC_T Cuts at: 0 409 1452 Size: 409 1043 BlpI GC'TnA_GC Cuts at: 0 143 1452 Size: 143 1309 Bme1580I G_kGCm'C Cuts at: 0 23 120 235 524 985 1452 Size: 23 97 115 289 461 467 Fragments arranged by size: 467 461 289 115 97 23 BmgBI CAC'GTC Cuts at: 0 516 1452 Size: 516 936 BmrI ACTGGGnnnn_n' Cuts at: 0 229 1112 1452 Size: 229 883 340 Fragments arranged by size: 883 340 229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 158 738 1191 1452 Size: 158 580 453 261 Fragments arranged by size: 580 453 261 158 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 269 391 717 883 1045 1078 1294 Size: 118 151 122 326 166 162 33 216 Cuts at: 1294 1452 Size: 158 Fragments arranged by size: 326 216 166 162 158 151 122 118 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1204 1452 Size: 1204 248 BsaAI yAC'GTr Cuts at: 0 18 1452 Size: 18 1434 BsaBI GATnn'nnATC Cuts at: 0 637 1452 Size: 637 815 BsaHI Gr'CG_yC Cuts at: 0 89 1452 Size: 89 1363 BsaWI w'CCGG_w Cuts at: 0 987 1409 1452 Size: 987 422 43 Fragments arranged by size: 987 422 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 436 466 1452 Size: 436 30 986 Fragments arranged by size: 986 436 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 241 711 1183 1291 1452 Size: 115 126 470 472 108 161 Fragments arranged by size: 472 470 161 126 115 108 BseYI C'CCAG_C Cuts at: 0 355 362 828 1086 1152 1452 Size: 355 7 466 258 66 300 Fragments arranged by size: 466 355 300 258 66 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 769 1452 Size: 262 507 683 Fragments arranged by size: 683 507 262 BsiHKAI G_wGCw'C Cuts at: 0 686 1043 1175 1228 1397 1452 Size: 686 357 132 53 169 55 Fragments arranged by size: 686 357 169 132 55 53 BsmBI CGTCTCn'nnnn_ Cuts at: 0 96 910 1452 Size: 96 814 542 Fragments arranged by size: 814 542 96 Bsp1286I G_dGCh'C Cuts at: 0 23 120 235 247 524 686 985 1043 Size: 23 97 115 12 277 162 299 58 Cuts at: 1043 1175 1228 1397 1452 Size: 132 53 169 55 Fragments arranged by size: 299 277 169 162 132 115 97 58 55 53 23 12 BsrBI CCG'CTC Cuts at: 0 731 1188 1452 Size: 731 457 264 Fragments arranged by size: 731 457 264 BsrDI GCAATG_nn' Cuts at: 0 306 737 1452 Size: 306 431 715 Fragments arranged by size: 715 431 306 BsrFI r'CCGG_y Cuts at: 0 1370 1427 1452 Size: 1370 57 25 Fragments arranged by size: 1370 57 25 BstXI CCAn_nnnn'nTGG Cuts at: 0 247 1452 Size: 247 1205 BstYI r'GATC_y Cuts at: 0 409 632 1246 1452 Size: 409 223 614 206 Fragments arranged by size: 614 409 223 206 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 327 1452 Size: 327 1125 DrdI GACnn_nn'nnGTC Cuts at: 0 88 1109 1452 Size: 88 1021 343 Fragments arranged by size: 1021 343 88 EaeI y'GGCC_r Cuts at: 0 779 839 1452 Size: 779 60 613 Fragments arranged by size: 779 613 60 EarI CTCTTCn'nnn_ Cuts at: 0 604 1452 Size: 604 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 449 508 1452 Size: 449 59 944 Fragments arranged by size: 944 449 59 EcoICRI GAG'CTC Cuts at: 0 1226 1452 Size: 1226 226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 269 391 717 883 1045 1078 1120 Size: 118 151 122 326 166 162 33 42 Cuts at: 1120 1294 1452 Size: 174 158 Fragments arranged by size: 326 174 166 162 158 151 122 118 42 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 100 1276 1452 Size: 100 1176 176 Fragments arranged by size: 1176 176 100 EcoO109I rG'GnC_Cy Cuts at: 0 1111 1302 1452 Size: 1111 191 150 Fragments arranged by size: 1111 191 150 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 615 1390 1452 Size: 615 775 62 Fragments arranged by size: 775 615 62 HaeII r_GCGC'y Cuts at: 0 369 720 1452 Size: 369 351 732 Fragments arranged by size: 732 369 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 668 700 1452 Size: 668 32 752 Fragments arranged by size: 752 668 32 HincII GTy'rAC Cuts at: 0 255 1452 Size: 255 1197 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 785 1452 Size: 785 667 MscI TGG'CCA Cuts at: 0 781 841 1452 Size: 781 60 611 Fragments arranged by size: 781 611 60 MslI CAynn'nnrTG Cuts at: 0 580 1452 Size: 580 872 MspA1I CmG'CkG Cuts at: 0 837 1023 1149 1452 Size: 837 186 126 303 Fragments arranged by size: 837 303 186 126 NdeI CA'TA_TG Cuts at: 0 510 963 1452 Size: 510 453 489 Fragments arranged by size: 510 489 453 NspI r_CATG'y Cuts at: 0 502 1452 Size: 502 950 PciI A'CATG_T Cuts at: 0 498 1452 Size: 498 954 PflMI CCAn_nnn'nTGG Cuts at: 0 369 1093 1452 Size: 369 724 359 Fragments arranged by size: 724 369 359 PpuMI rG'GwC_Cy Cuts at: 0 1111 1302 1452 Size: 1111 191 150 Fragments arranged by size: 1111 191 150 PshAI GACnn'nnGTC Cuts at: 0 199 1452 Size: 199 1253 PstI C_TGCA'G Cuts at: 0 794 1452 Size: 794 658 PvuII CAG'CTG Cuts at: 0 837 1023 1149 1452 Size: 837 186 126 303 Fragments arranged by size: 837 303 186 126 SacI G_AGCT'C Cuts at: 0 1228 1452 Size: 1228 224 SfcI C'TryA_G Cuts at: 0 790 1452 Size: 790 662 SmaI CCC'GGG Cuts at: 0 24 919 1130 1452 Size: 24 895 211 322 Fragments arranged by size: 895 322 211 24 SmlI C'TyrA_G Cuts at: 0 1183 1452 Size: 1183 269 StyI C'CwwG_G Cuts at: 0 1432 1439 1452 Size: 1432 7 13 Fragments arranged by size: 1432 13 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 842 1100 1452 Size: 842 258 352 Fragments arranged by size: 842 352 258 TatI w'GTAC_w Cuts at: 0 293 1452 Size: 293 1159 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 214 246 1452 Size: 214 32 1206 Fragments arranged by size: 1206 214 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 534 849 1090 1452 Size: 534 315 241 362 Fragments arranged by size: 534 362 315 241 XmaI C'CCGG_G Cuts at: 0 22 917 1128 1452 Size: 22 895 211 324 Fragments arranged by size: 895 324 211 22 XmnI GAAnn'nnTTC Cuts at: 0 500 1452 Size: 500 952 ZraI GAC'GTC Cuts at: 0 90 1452 Size: 90 1362 Enzymes that do cut: AatII AcuI AfeI AflIII AlwNI AvaI BanI BanII BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstXI BstYI BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NdeI NspI PciI PflMI PpuMI PshAI PstI PvuII SacI SfcI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BmtI Bpu10I BsaI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII EagI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI