################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AlwNI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BsrFI BsrGI BssHII BstEII BstPI CelII Cfr10I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FseI HaeII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 190 GTATAC GT^[AC][GT]AC AcyI 676 GACGCC G[GA]^CG[CT]C AflIII 306 ACATGT A^C[GA][CT]GT Alw44I 477 GTGCAC G^TGCAC AocI 340 CCTCAGG CC^T[ACGT]AGG ApaI 1924 GGGCCC GGGCC^C ApaLI 477 GTGCAC G^TGCAC AviII 1445 TGCGCA TGC^GCA BbsI 1419 GTCTTC GAAGAC(2/6) BpuAI 1419 GTCTTC GAAGAC(2/6) BsaHI 676 GACGCC G[GA]^CG[CT]C BsgI 923 GTGCAG GTGCAG(16/14) BsmBI 159 CGTCTC CGTCTC(1/5) BsmI 599 GCATTC GAATGC(1/-1) BspDI 200 ATCGAT AT^CGAT BspHI 1284 TCATGA T^CATGA BspLU11I 306 ACATGT A^CATGT BsrBI 1342 GAGCGG CCGCTC(-3/-3) BsrDI 885 CATTGC GCAATG(2/0) BssSI 1895 CTCGTG CACGAG(-5/-1) Bst1107I 190 GTATAC GTA^TAC BstAPI 1255 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1908 TTCGAA TT^CGAA BstXI 293 CCAAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 190 GTATAC GTA^TAC Bsu36I 340 CCTCAGG CC^T[ACGT]AGG ClaI 200 ATCGAT AT^CGAT Eco81I 340 CCTCAGG CC^T[ACGT]AGG EcoT14I 689 CCTTGG C^C[AT][AT]GG FspI 1445 TGCGCA TGC^GCA Hin1I 676 GACGCC G[GA]^CG[CT]C MslI 460 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 1256 CATATG CA^TATG NspV 1908 TTCGAA TT^CGAA PflMI 1093 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1722 GGGACCC [GA]G^G[AT]CC[CT] RcaI 1284 TCATGA T^CATGA SacI 867 GAGCTC GAGCT^C SapI 869 GCTCTTC GCTCTTC(1/4) ScaI 1780 AGTACT AGT^ACT SfuI 1908 TTCGAA TT^CGAA SpeI 1051 ACTAGT A^CTAGT StyI 689 CCTTGG C^C[AT][AT]GG Van91I 1093 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 1177 CGCG CG^CG 1179 CGCG AlwI 633 GATCC GGATC(4/5) 1630 GATCC Asp700I 346 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2044 GAATTTGTTC AvaI 44 CTCGAG C^[CT]CG[GA]G 2097 CTCGAG BanII 867 GAGCTC G[GA]GC[CT]^C 1924 GGGCCC BglII 393 AGATCT A^GATCT 2101 AGATCT BsmFI 1722 GGGAC GGGAC(10/14) 1900 GTCCC BsoBI 44 CTCGAG C^[CT]CG[GA]G 2097 CTCGAG BspMI 889 GCAGGT ACCTGC(4/8) 1399 GCAGGT BstUI 1177 CGCG CG^CG 1179 CGCG CfoI 1178 GCGC GCG^C 1446 GCGC DraII 1722 GGGACCC [GA]G^G[ACGT]CC[CT] 1923 AGGGCCC EaeI 122 CGGCCA [CT]^GGCC[GA] 2125 CGGCCA EcoO109I 1722 GGGACCC [GA]G^G[ACGT]CC[CT] 1923 AGGGCCC HhaI 1178 GCGC GCG^C 1446 GCGC HinP1I 1178 GCGC G^CGC 1446 GCGC HincII 673 GTTGAC GT[CT]^[GA]AC 906 GTCAAC HindII 673 GTTGAC GT[CT]^[GA]AC 906 GTCAAC MaeII 158 ACGT A^CGT 1620 ACGT MvnI 1177 CGCG CG^CG 1179 CGCG PaeR7I 44 CTCGAG C^TCGAG 2097 CTCGAG PleI 1533 GACTC GAGTC(4/5) 952 GAGTC PstI 565 CTGCAG CTGCA^G 1463 CTGCAG SfaNI 1836 GATGC GCATC(5/9) 198 GCATC SmlI 44 CTCGAG C^T[CT][GA]AG 2097 CTCGAG SphI 778 GCATGC GCATG^C 1185 GCATGC XcmI 680 CCACTGTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1593 CCAACTTCCAGTTGG XhoI 44 CTCGAG C^TCGAG 2097 CTCGAG XmnI 346 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2044 GAATTTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaII BbvI BclI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT22I FbaI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII