(Circular) (Six-base) MAPSORT of: pFN21AE2261.seq Check: 1886 from: 1 to: 6883 pFN21AE2261 6883 bp With 182 enzymes: SgfI * March 28, 2012 12:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3395 3395 Size: 6883 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4693 278 Size: 53 83 186 936 3157 2468 Fragments arranged by size: 3157 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2196 4226 1937 Size: 259 2030 4594 Fragments arranged by size: 4594 2030 259 Acc65I G'GTAC_C Cuts at: 4204 4204 Size: 6883 AclI AA'CG_TT Cuts at: 1563 5010 5383 1563 Size: 3447 373 3063 Fragments arranged by size: 3447 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2823 2862 3288 3756 4946 6330 6598 Size: 491 700 39 426 468 1190 1384 268 Cuts at: 6598 1632 Size: 1917 Fragments arranged by size: 1917 1384 1190 700 491 468 426 268 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6686 AflIII A'CryG_T Cuts at: 1203 2311 5782 6759 1203 Size: 1108 3471 977 1327 Fragments arranged by size: 3471 1327 1108 977 AgeI A'CCGG_T Cuts at: 5714 5714 Size: 6883 AhdI GACnn_n'nnGTC Cuts at: 5612 5612 Size: 6883 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6883 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2441 1184 Size: 1257 5626 AlwNI CAG_nnn'CTG Cuts at: 4626 6198 4626 Size: 1572 5311 ApaI G_GGCC'C Cuts at: 3933 3933 Size: 6883 ApaLI G'TGCA_C Cuts at: 2482 4940 6096 2482 Size: 2458 1156 3269 Fragments arranged by size: 3269 2458 1156 ApoI r'AATT_y Cuts at: 1493 2245 3064 3805 4049 4192 4418 4570 6679 Size: 752 819 741 244 143 226 152 2109 Cuts at: 6679 1493 Size: 1697 Fragments arranged by size: 2109 1697 819 752 741 244 226 152 143 AseI AT'TA_AT Cuts at: 160 1032 5437 160 Size: 872 4405 1606 Fragments arranged by size: 4405 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6883 AvaI C'yCGr_G Cuts at: 1870 1943 2049 4102 4208 1870 Size: 73 106 2053 106 4545 Fragments arranged by size: 4545 2053 106 106 73 BamHI G'GATC_C Cuts at: 4213 4580 4213 Size: 367 6516 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4204 5659 618 Size: 325 217 627 2417 1455 1842 Fragments arranged by size: 2417 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2876 3933 4202 1391 Size: 1485 1057 269 4072 Fragments arranged by size: 4072 1485 1057 269 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3417 928 Size: 971 1518 4394 Fragments arranged by size: 4394 1518 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5074 5108 1903 Size: 34 3137 34 3678 Fragments arranged by size: 3678 3137 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4775 5985 4775 Size: 1210 5673 BclI T'GATC_A Cuts at: 1548 2455 3085 3760 1548 Size: 907 630 675 4671 Fragments arranged by size: 4671 907 675 630 BfrBI ATG'CAT Cuts at: 2203 3116 3260 2203 Size: 913 144 5826 Fragments arranged by size: 5826 913 144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5494 136 Size: 107 122 71 631 605 3822 1525 Fragments arranged by size: 3822 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2398 4106 6878 2398 Size: 1708 2772 2403 Fragments arranged by size: 2772 2403 1708 BlpI GC'TnA_GC Cuts at: 4300 4300 Size: 6883 Bme1580I G_kGCm'C Cuts at: 1790 2486 3933 4015 4944 6100 1790 Size: 696 1447 82 929 1156 2573 Fragments arranged by size: 2573 1447 1156 929 696 82 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6883 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2636 3392 3624 Size: 416 53 83 399 24 1219 756 232 Cuts at: 3624 3945 4614 5572 442 Size: 321 669 958 1753 Fragments arranged by size: 1753 1219 958 756 669 416 399 321 232 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6883 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2241 3245 3765 5543 1239 Size: 1002 1004 520 1778 2579 Fragments arranged by size: 2579 1778 1004 1002 520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5008 5873 6171 5008 Size: 865 298 5720 Fragments arranged by size: 5720 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5546 882 Size: 617 4047 2219 Fragments arranged by size: 4047 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4674 493 Size: 711 3470 2702 Fragments arranged by size: 3470 2702 711 BsaBI GATnn'nnATC Cuts at: 6877 6877 Size: 6883 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2682 4690 5072 5707 Size: 53 83 186 936 1149 2008 382 635 Cuts at: 5707 275 Size: 1451 Fragments arranged by size: 2008 1451 1149 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5316 5714 5988 6135 6546 5316 Size: 398 274 147 411 5653 Fragments arranged by size: 5653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3738 3768 1178 Size: 30 165 30 2335 30 4293 Fragments arranged by size: 4293 2335 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 3452 4090 4146 1186 Size: 1117 1149 638 56 3923 Fragments arranged by size: 3923 1149 1117 638 56 BseYI C'CCAG_C Cuts at: 3887 6086 6582 3887 Size: 2199 496 4188 Fragments arranged by size: 4188 2199 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2949 1163 Size: 573 1213 5097 Fragments arranged by size: 5097 1213 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4333 5094 6122 1540 Size: 275 186 2332 761 1028 2301 Fragments arranged by size: 2332 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2486 2876 3522 4944 5029 6100 2486 Size: 390 646 1422 85 1071 3269 Fragments arranged by size: 3269 1422 1071 646 390 85 BsmI GAATG_Cn' Cuts at: 2604 4394 4487 2604 Size: 1790 93 5000 Fragments arranged by size: 5000 1790 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2170 2170 Size: 6883 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2486 2876 3522 3933 4015 4202 4944 Size: 399 696 390 646 411 82 187 742 Cuts at: 4944 5029 6100 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 696 646 411 399 390 187 85 82 BspEI T'CCGG_A Cuts at: 6546 6546 Size: 6883 BspHI T'CATG_A Cuts at: 3289 4772 6502 3289 Size: 1483 1730 3670 Fragments arranged by size: 3670 1730 1483 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2885 3395 4238 844 Size: 2041 510 843 3489 Fragments arranged by size: 3489 2041 843 510 BsrBI CCG'CTC Cuts at: 1394 1823 3349 4770 1394 Size: 429 1526 1421 3507 Fragments arranged by size: 3507 1526 1421 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2889 5378 5552 65 Size: 1183 1641 2489 174 1396 Fragments arranged by size: 2489 1641 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5527 5714 1700 Size: 110 3717 187 2869 Fragments arranged by size: 3717 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6883 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6883 BssSI C'ACGA_G Cuts at: 3900 4943 5955 3900 Size: 1043 1012 4828 Fragments arranged by size: 4828 1043 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3266 794 Size: 2472 4411 BstBI TT'CG_AA Cuts at: 3914 5687 3914 Size: 1773 5110 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6883 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 6883 BstYI r'GATC_y Cuts at: 1972 2398 2637 4106 4213 4580 4965 4982 6434 Size: 426 239 1469 107 367 385 17 1452 Cuts at: 6434 6520 6532 6878 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1469 1452 426 385 367 346 239 107 86 17 12 BstZ17I GTA'TAC Cuts at: 2197 2197 Size: 6883 Bsu36I CC'TnA_GG Cuts at: 2346 4179 2346 Size: 1833 5050 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5392 Fragments arranged by size: 5392 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6144 Fragments arranged by size: 6144 683 56 BtsI GCAGTG_nn' Cuts at: 2184 2937 4399 5193 5213 2184 Size: 753 1462 794 20 3854 Fragments arranged by size: 3854 1462 794 753 20 ClaI AT'CG_AT Cuts at: 1554 2206 1554 Size: 652 6231 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6848 DraI TTT'AAA Cuts at: 4187 4187 Size: 6883 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5890 809 Size: 507 4574 1802 Fragments arranged by size: 4574 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 4130 4330 Size: 54 1425 50 275 18 297 2003 200 Cuts at: 4330 5219 6848 8 Size: 889 1629 43 Fragments arranged by size: 2003 1629 1425 889 297 275 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4330 1537 Size: 275 2518 4090 Fragments arranged by size: 4090 2518 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2881 2949 3209 3709 3732 3802 4813 1353 Size: 1528 68 260 500 23 70 1011 3423 Fragments arranged by size: 3423 1528 1011 500 260 70 68 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5470 5844 5990 387 Size: 1064 138 3881 374 146 1280 Fragments arranged by size: 3881 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2874 2874 Size: 6883 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2241 2823 2862 3245 3288 3756 Size: 393 491 118 582 39 383 43 468 Cuts at: 3756 3765 4946 5543 6330 6598 1239 Size: 9 1181 597 787 268 1524 Fragments arranged by size: 1524 1181 787 597 582 491 468 393 383 268 118 43 39 9 EcoNI CCTnn'n_nnAGG Cuts at: 4178 4178 Size: 6883 EcoO109I rG'GnC_Cy Cuts at: 3728 3929 3728 Size: 201 6682 EcoRI G'AATT_C Cuts at: 4192 4570 4192 Size: 378 6505 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2394 2394 Size: 6883 FspI TGC'GCA Cuts at: 3452 5389 3452 Size: 1937 4946 FspAI rTGC'GCAy Cuts at: 3452 3452 Size: 6883 HaeII r_GCGC'y Cuts at: 1678 6030 1678 Size: 4352 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5524 5556 5598 5630 1373 Size: 32 135 32 3952 32 42 32 2626 Fragments arranged by size: 3952 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2680 2913 4227 4473 669 Size: 988 281 742 233 1314 246 3079 Fragments arranged by size: 3079 1314 988 742 281 246 233 HindIII A'AGCT_T Cuts at: 748 748 Size: 6883 HpaI GTT'AAC Cuts at: 4473 4473 Size: 6883 KpnI G_GTAC'C Cuts at: 4208 4208 Size: 6883 MfeI C'AATT_G Cuts at: 2171 2451 2629 4482 2171 Size: 280 178 1853 4572 Fragments arranged by size: 4572 1853 280 178 MluI A'CGCG_T Cuts at: 6759 6759 Size: 6883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5997 6181 1305 Size: 4692 184 2007 Fragments arranged by size: 4692 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5061 Fragments arranged by size: 5061 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2469 4841 5200 5359 518 Size: 648 1303 2372 359 159 2042 Fragments arranged by size: 2372 2042 1303 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 3473 3891 4299 4976 6124 6369 1606 Size: 453 1414 418 408 677 1148 245 2120 Fragments arranged by size: 2120 1414 1148 677 453 418 408 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5392 Fragments arranged by size: 5392 939 552 NdeI CA'TA_TG Cuts at: 387 3262 387 Size: 2875 4008 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6883 NotI GC'GGCC_GC Cuts at: 4330 4330 Size: 6883 NsiI A_TGCA'T Cuts at: 2205 3118 3262 2205 Size: 913 144 5826 Fragments arranged by size: 5826 913 144 NspI r_CATG'y Cuts at: 1132 1229 2203 2315 2787 2911 3194 4241 5786 Size: 97 974 112 472 124 283 1047 1545 Cuts at: 5786 1132 Size: 2229 Fragments arranged by size: 2229 1545 1047 974 472 283 124 112 97 PasI CC'CwG_GG Cuts at: 1348 4015 1348 Size: 2667 4216 PciI A'CATG_T Cuts at: 2311 5782 2311 Size: 3471 3412 PflMI CCAn_nnn'nTGG Cuts at: 1108 3104 1108 Size: 1996 4887 PmeI GTTT'AAAC Cuts at: 4187 4187 Size: 6883 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4952 4984 6465 6497 4952 Size: 32 1481 32 5338 Fragments arranged by size: 5338 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3728 3728 Size: 6883 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6883 PsiI TTA'TAA Cuts at: 4453 4453 Size: 6883 PspOMI G'GGCC_C Cuts at: 3929 3929 Size: 6883 PstI C_TGCA'G Cuts at: 830 2574 3472 4235 830 Size: 1744 898 763 3478 Fragments arranged by size: 3478 1744 898 763 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6883 PvuII CAG'CTG Cuts at: 2059 3473 3891 2059 Size: 1414 418 5051 Fragments arranged by size: 5051 1414 418 SacI G_AGCT'C Cuts at: 2876 2876 Size: 6883 SalI G'TCGA_C Cuts at: 1936 4225 1936 Size: 2289 4594 SanDI GG'GwC_CC Cuts at: 3728 3728 Size: 6883 SapI GCTCTTCn'nnn_ Cuts at: 2881 2881 Size: 6883 SbfI CC_TGCA'GG Cuts at: 4235 4235 Size: 6883 ScaI AGT'ACT Cuts at: 3787 5131 6843 3787 Size: 1344 1712 3827 Fragments arranged by size: 3827 1712 1344 SfcI C'TryA_G Cuts at: 826 1045 2190 2570 2729 3468 3489 3679 4231 Size: 219 1145 380 159 739 21 190 552 Cuts at: 4231 5366 6047 6238 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1145 1135 739 681 552 380 219 191 190 159 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6883 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6883 SmaI CCC'GGG Cuts at: 1872 4210 1872 Size: 2338 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2049 4102 4987 5888 6150 820 Size: 197 926 106 2053 885 901 262 1553 Fragments arranged by size: 2053 1553 926 901 885 262 197 106 SnaBI TAC'GTA Cuts at: 493 4674 493 Size: 4181 2702 SpeI A'CTAG_T Cuts at: 152 3056 152 Size: 2904 3979 SphI G_CATG'C Cuts at: 1132 2787 3194 4241 1132 Size: 1655 407 1047 3774 Fragments arranged by size: 3774 1655 1047 407 SspI AAT'ATT Cuts at: 5 52 4807 5 Size: 47 4755 2081 Fragments arranged by size: 4755 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6883 StyI C'CwwG_G Cuts at: 513 1065 2004 2694 4322 513 Size: 552 939 690 1628 3074 Fragments arranged by size: 3074 1628 939 690 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2475 2832 3558 4074 4910 4927 5080 Size: 1255 895 357 726 516 836 17 153 Cuts at: 5080 5265 5710 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 895 836 726 516 445 357 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 3785 5129 Size: 274 80 33 51 1443 597 1211 1344 Cuts at: 5129 6841 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1344 1211 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2235 2267 3578 3610 3738 3770 6843 6875 2235 Size: 32 1311 32 128 32 3073 32 2243 Fragments arranged by size: 3073 2243 1311 128 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6883 XbaI T'CTAG_A Cuts at: 4219 4219 Size: 6883 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2692 3605 2692 Size: 913 5970 XhoI C'TCGA_G Cuts at: 1943 2049 4102 1943 Size: 106 2053 4724 Fragments arranged by size: 4724 2053 106 XmaI C'CCGG_G Cuts at: 1870 4208 1870 Size: 2338 4545 XmnI GAAnn'nnTTC Cuts at: 2355 4053 5012 2355 Size: 1698 959 4226 Fragments arranged by size: 4226 1698 959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4691 276 Size: 53 83 186 936 3157 2468 Fragments arranged by size: 3157 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI