(Linear) (Six-base) MAPSORT of: KIEE2261.seq Check: 8872 from: 1 to: 2178 KIEE2261.orf With 182 enzymes: SgfI * March 22, 2012 16:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1390 2178 Size: 1390 788 AccI GT'mk_AC Cuts at: 0 191 2178 Size: 191 1987 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 818 857 1283 1751 2178 Size: 118 700 39 426 468 427 Fragments arranged by size: 700 468 427 426 118 39 AflIII A'CryG_T Cuts at: 0 306 2178 Size: 306 1872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 436 2178 Size: 436 1742 ApaI G_GGCC'C Cuts at: 0 1928 2178 Size: 1928 250 ApaLI G'TGCA_C Cuts at: 0 477 2178 Size: 477 1701 ApoI r'AATT_y Cuts at: 0 240 1059 1800 2044 2178 Size: 240 819 741 244 134 Fragments arranged by size: 819 741 244 240 134 AvaI C'yCGr_G Cuts at: 0 44 2097 2178 Size: 44 2053 81 Fragments arranged by size: 2053 81 44 BanII G_rGCy'C Cuts at: 0 871 1928 2178 Size: 871 1057 250 Fragments arranged by size: 1057 871 250 BbsI GAAGACnn'nnnn_ Cuts at: 0 1412 2178 Size: 1412 766 BclI T'GATC_A Cuts at: 0 450 1080 1755 2178 Size: 450 630 675 423 Fragments arranged by size: 675 630 450 423 BfrBI ATG'CAT Cuts at: 0 198 1111 1255 2178 Size: 198 913 144 923 Fragments arranged by size: 923 913 198 144 BglII A'GATC_T Cuts at: 0 393 2101 2178 Size: 393 1708 77 Fragments arranged by size: 1708 393 77 Bme1580I G_kGCm'C Cuts at: 0 481 1928 2010 2178 Size: 481 1447 82 168 Fragments arranged by size: 1447 481 168 82 BmrI ACTGGGnnnn_n' Cuts at: 0 631 1387 1619 1940 2178 Size: 631 756 232 321 238 Fragments arranged by size: 756 631 321 238 232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 1240 1760 2178 Size: 236 1004 520 418 Fragments arranged by size: 1004 520 418 236 BsaHI Gr'CG_yC Cuts at: 0 677 2178 Size: 677 1501 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1733 1763 2178 Size: 1733 30 415 Fragments arranged by size: 1733 415 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 298 1447 2085 2141 2178 Size: 298 1149 638 56 37 Fragments arranged by size: 1149 638 298 56 37 BseYI C'CCAG_C Cuts at: 0 1882 2178 Size: 1882 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 944 2178 Size: 944 1234 BsiHKAI G_wGCw'C Cuts at: 0 481 871 1517 2178 Size: 481 390 646 661 Fragments arranged by size: 661 646 481 390 BsmI GAATG_Cn' Cuts at: 0 599 2178 Size: 599 1579 BsmBI CGTCTCn'nnnn_ Cuts at: 0 165 2178 Size: 165 2013 Bsp1286I G_dGCh'C Cuts at: 0 481 871 1517 1928 2010 2178 Size: 481 390 646 411 82 168 Fragments arranged by size: 646 481 411 390 168 82 BspHI T'CATG_A Cuts at: 0 1284 2178 Size: 1284 894 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 880 1390 2178 Size: 880 510 788 Fragments arranged by size: 880 788 510 BsrBI CCG'CTC Cuts at: 0 1344 2178 Size: 1344 834 BsrDI GCAATG_nn' Cuts at: 0 884 2178 Size: 884 1294 BssSI C'ACGA_G Cuts at: 0 1895 2178 Size: 1895 283 BstAPI GCAn_nnn'nTGC Cuts at: 0 1261 2178 Size: 1261 917 BstBI TT'CG_AA Cuts at: 0 1909 2178 Size: 1909 269 BstXI CCAn_nnnn'nTGG Cuts at: 0 300 2178 Size: 300 1878 BstYI r'GATC_y Cuts at: 0 393 632 2101 2178 Size: 393 239 1469 77 Fragments arranged by size: 1469 393 239 77 BstZ17I GTA'TAC Cuts at: 0 192 2178 Size: 192 1986 Bsu36I CC'TnA_GG Cuts at: 0 341 2178 Size: 341 1837 BtsI GCAGTG_nn' Cuts at: 0 179 932 2178 Size: 179 753 1246 Fragments arranged by size: 1246 753 179 ClaI AT'CG_AT Cuts at: 0 201 2178 Size: 201 1977 EaeI y'GGCC_r Cuts at: 0 122 2125 2178 Size: 122 2003 53 Fragments arranged by size: 2003 122 53 EarI CTCTTCn'nnn_ Cuts at: 0 876 944 1204 1704 1727 1797 2178 Size: 876 68 260 500 23 70 381 Fragments arranged by size: 876 500 381 260 70 68 23 EcoICRI GAG'CTC Cuts at: 0 869 2178 Size: 869 1309 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 236 818 857 1240 1283 1751 1760 Size: 118 118 582 39 383 43 468 9 Cuts at: 1760 2178 Size: 418 Fragments arranged by size: 582 468 418 383 118 118 43 39 9 EcoO109I rG'GnC_Cy Cuts at: 0 1723 1924 2178 Size: 1723 201 254 Fragments arranged by size: 1723 254 201 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 389 2178 Size: 389 1789 FspI TGC'GCA Cuts at: 0 1447 2178 Size: 1447 731 FspAI rTGC'GCAy Cuts at: 0 1447 2178 Size: 1447 731 HincII GTy'rAC Cuts at: 0 675 908 2178 Size: 675 233 1270 Fragments arranged by size: 1270 675 233 MfeI C'AATT_G Cuts at: 0 166 446 624 2178 Size: 166 280 178 1554 Fragments arranged by size: 1554 280 178 166 MslI CAynn'nnrTG Cuts at: 0 464 2178 Size: 464 1714 MspA1I CmG'CkG Cuts at: 0 54 1468 1886 2178 Size: 54 1414 418 292 Fragments arranged by size: 1414 418 292 54 NdeI CA'TA_TG Cuts at: 0 1257 2178 Size: 1257 921 NsiI A_TGCA'T Cuts at: 0 200 1113 1257 2178 Size: 200 913 144 921 Fragments arranged by size: 921 913 200 144 NspI r_CATG'y Cuts at: 0 198 310 782 906 1189 2178 Size: 198 112 472 124 283 989 Fragments arranged by size: 989 472 283 198 124 112 PasI CC'CwG_GG Cuts at: 0 2010 2178 Size: 2010 168 PciI A'CATG_T Cuts at: 0 306 2178 Size: 306 1872 PflMI CCAn_nnn'nTGG Cuts at: 0 1099 2178 Size: 1099 1079 PpuMI rG'GwC_Cy Cuts at: 0 1723 2178 Size: 1723 455 PspOMI G'GGCC_C Cuts at: 0 1924 2178 Size: 1924 254 PstI C_TGCA'G Cuts at: 0 569 1467 2178 Size: 569 898 711 Fragments arranged by size: 898 711 569 PvuII CAG'CTG Cuts at: 0 54 1468 1886 2178 Size: 54 1414 418 292 Fragments arranged by size: 1414 418 292 54 SacI G_AGCT'C Cuts at: 0 871 2178 Size: 871 1307 SanDI GG'GwC_CC Cuts at: 0 1723 2178 Size: 1723 455 SapI GCTCTTCn'nnn_ Cuts at: 0 876 2178 Size: 876 1302 ScaI AGT'ACT Cuts at: 0 1782 2178 Size: 1782 396 SfcI C'TryA_G Cuts at: 0 185 565 724 1463 1484 1674 2178 Size: 185 380 159 739 21 190 504 Fragments arranged by size: 739 504 380 190 185 159 21 SmlI C'TyrA_G Cuts at: 0 44 2097 2178 Size: 44 2053 81 Fragments arranged by size: 2053 81 44 SpeI A'CTAG_T Cuts at: 0 1051 2178 Size: 1051 1127 SphI G_CATG'C Cuts at: 0 782 1189 2178 Size: 782 407 989 Fragments arranged by size: 989 782 407 StyI C'CwwG_G Cuts at: 0 689 2178 Size: 689 1489 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 470 827 1553 2069 2178 Size: 470 357 726 516 109 Fragments arranged by size: 726 516 470 357 109 TatI w'GTAC_w Cuts at: 0 569 1780 2178 Size: 569 1211 398 Fragments arranged by size: 1211 569 398 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 230 262 1573 1605 1733 1765 2178 Size: 230 32 1311 32 128 32 413 Fragments arranged by size: 1311 413 230 128 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 687 1600 2178 Size: 687 913 578 Fragments arranged by size: 913 687 578 XhoI C'TCGA_G Cuts at: 0 44 2097 2178 Size: 44 2053 81 Fragments arranged by size: 2053 81 44 XmnI GAAnn'nnTTC Cuts at: 0 350 2048 2178 Size: 350 1698 130 Fragments arranged by size: 1698 350 130 Enzymes that do cut: AarI AccI AcuI AflIII AlfI ApaI ApaLI ApoI AvaI BanII BbsI BclI BfrBI BglII Bme1580I BmrI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I FalI FspI FspAI HincII MfeI MslI MspA1I NdeI NsiI NspI PasI PciI PflMI PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI SfcI SmlI SpeI SphI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BsrFI BsrGI BssHII BstEII BtgI BtgZI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI HaeII Hin4I HindIII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI