################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AfaI 140 GTAC GT^AC AlwI 339 GATCC GGATC(4/5) AlwNI 85 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 252 GGTCC G^G[AT]CC BalI 53 TGGCCA TGG^CCA BglI 46 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 387 CTGCAC GTGCAG(16/14) BstYI 338 AGATCC [GA]^GATC[CT] DraII 251 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 53 TGGCCA [CT]^GGCC[GA] EarI 211 CTCTTC CTCTTC(1/4) EcoO109I 251 AGGTCCC [GA]G^G[ACGT]CC[CT] HindIII 229 AAGCTT A^AGCTT HinfI 209 GACTC G^A[ACGT]TC Ksp632I 211 CTCTTC CTCTTC(1/4) MflI 338 AGATCC [GA]^GATC[CT] MluNI 53 TGGCCA TGG^CCA MscI 53 TGGCCA TGG^CCA MseI 350 TTAA T^TAA MslI 151 CACACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 85 CAGCTG C[AC]G^C[GT]G NlaIII 144 CATG CATG^ NlaIV 252 GGTCCC GG[ACGT]^[ACGT]CC PleI 209 GACTC GAGTC(4/5) PpuMI 251 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 85 CAGCTG CAG^CTG RsaI 140 GTAC GT^AC Tru9I 350 TTAA T^TAA XcmI 332 CCAAGAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 338 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BbvI 245 GCAGC GCAGC(8/12) 87 GCTGC BsrI 365 ACTGG ACTGG(1/-1) 155 CCAGT Cfr13I 252 GGTCC G^G[ACGT]CC 372 GGCCC DdeI 257 CTCAG C^T[ACGT]AG 392 CTGAG Fnu4HI 87 GCTGC GC^[ACGT]GC 245 GCAGC HaeIII 54 GGCC GG^CC 372 GGCC ItaI 87 GCTGC GC^[ACGT]GC 245 GCAGC MaeIII 275 GTGAC ^GT[ACGT]AC 287 GTGAC MboII 336 GAAGA GAAGA(8/7) 212 TCTTC Sau96I 252 GGTCC G^G[ACGT]CC 372 GGCCC SfaNI 32 GCATC GCATC(5/9) 116 GCATC TseI 87 GCTGC G^C[AT]GC 245 GCAGC Tsp45I 275 GTGAC ^GT[GC]AC 287 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BsmFI BssKI BstF5I BstNI DpnII EcoRII FokI MboI MnlI MvaI MwoI NdeII Sau3AI ScrFI Tsp509I TspRI