################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AvrII BalI BbeI BbrPI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 885 GGTACC G^GTACC AlwNI 1078 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 885 GGTACC G^GTACC AspHI 47 GAGCAC G[AT]GC[AT]^C AvaII 1012 GGACC G^G[AT]CC AviII 951 TGCGCA TGC^GCA BanII 1139 GAGCCC G[GA]GC[CT]^C BbsI 1170 GAAGAC GAAGAC(2/6) BbvCI 523 GCTGAGG CCTCAGC(-5/-2) BcnI 1091 CCCGG CC^[GC]GG BglII 265 AGATCT A^GATCT BpuAI 1170 GAAGAC GAAGAC(2/6) BsaI 194 GGTCTC GGTCTC(1/5) BseRI 53 CTCCTC GAGGAG(10/8) BsiHKAI 47 GAGCAC G[AT]GC[AT]^C BsmBI 98 GAGACG CGTCTC(1/5) Bsp1407I 1257 TGTACA T^GTACA BspMI 882 GCAGGT ACCTGC(4/8) BsrBI 190 GAGCGG CCGCTC(-3/-3) BsrDI 1261 CATTGC GCAATG(2/0) BsrGI 1257 TGTACA T^GTACA BstAPI 1077 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 952 GCGC GCG^C DrdI 948 GACTGCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 240 TGGCCG [CT]^GGCC[GA] EcoRI 32 GAATTC G^AATTC FspI 951 TGCGCA TGC^GCA HapII 1092 CCGG C^CGG HhaI 952 GCGC GCG^C HinP1I 952 GCGC G^CGC HpaII 1092 CCGG C^CGG KpnI 885 GGTACC GGTAC^C MaeIII 575 GTAAC ^GT[ACGT]AC MspI 1092 CCGG C^CGG NciI 1091 CCCGG CC^[GC]GG PpuMI 1011 AGGACCC [GA]G^G[AT]CC[CT] SbfI 1047 CCTGCAGG CCTGCA^GG SmlI 453 CTCAAG C^T[CT][GA]AG Sse8387I 1047 CCTGCAGG CCTGCA^GG SspBI 1257 TGTACA T^GTACA StuI 412 AGGCCT AGG^CCT TaqI 57 TCGA T^CGA TthHB8I 57 TCGA T^CGA XcmI 1144 CCAGACATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Asp700I 28 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 245 GAAAGGATTC BamHI 607 GGATCC G^GATCC 1193 GGATCC BanI 361 GGTGCC G^G[CT][GA]CC 885 GGTACC BcgI 102 CGATCATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 942 CGAAGAGACTGC BfaI 556 CTAG C^TAG 1009 CTAG BmyI 47 GAGCAC G[AGT]GC[ACT]^C 1139 GAGCCC BpmI 299 CTCCAG CTGGAG(16/14) 1223 CTCCAG BsaJI 1090 CCCCGG C^C[ACGT][ACGT]GG 1154 CCTGGG Bsp1286I 47 GAGCAC G[AGT]GC[ACT]^C 1139 GAGCCC BstNI 358 CCTGG CC^[AT]GG 1154 CCTGG EarI 171 CTCTTC CTCTTC(1/4) 943 GAAGAG EcoRII 358 CCTGG ^CC[AT]GG 1154 CCTGG HgaI 982 GACGC GACGC(5/10) 354 GCGTC Ksp632I 171 CTCTTC CTCTTC(1/4) 943 GAAGAG MaeI 556 CTAG C^TAG 1009 CTAG MslI 64 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 903 CACTGAAATG MvaI 358 CCTGG CC^[AT]GG 1154 CCTGG PleI 649 GAGTC GAGTC(4/5) 859 GAGTC XmnI 28 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 245 GAAAGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MboII MflI MnlI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp509I TspRI XhoII