(Circular) (Six-base) MAPSORT of: pFN21AE1922.seq Check: 6705 from: 1 to: 6007 pFN21AE1922 6007 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 2890 3328 2890 Size: 438 5569 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3191 4070 5454 5722 1632 Size: 1559 879 1384 268 1917 Fragments arranged by size: 1917 1559 1384 879 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 4906 5883 1203 Size: 3703 977 1327 Fragments arranged by size: 3703 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6007 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 3088 3750 5322 3088 Size: 662 1572 3773 Fragments arranged by size: 3773 1572 662 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 2037 2592 2848 3235 3316 3542 3694 5803 Size: 544 555 256 387 81 226 152 2109 Cuts at: 5803 1493 Size: 1697 Fragments arranged by size: 2109 1697 555 544 387 256 226 152 81 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 3332 1870 Size: 73 1389 4545 Fragments arranged by size: 4545 1389 73 BamHI G'GATC_C Cuts at: 2612 3198 3337 3704 2612 Size: 586 139 367 4915 Fragments arranged by size: 4915 586 367 139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2890 3328 4783 618 Size: 325 217 627 579 524 438 1455 1842 Fragments arranged by size: 1842 1455 627 579 524 438 325 217 BanII G_rGCy'C Cuts at: 1391 3148 3326 1391 Size: 1757 178 4072 Fragments arranged by size: 4072 1757 178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3182 928 Size: 971 1283 3753 Fragments arranged by size: 3753 1283 971 BbvCI CC'TCA_GC Cuts at: 2529 2529 Size: 6007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2096 2130 2936 2970 4198 4232 1903 Size: 34 159 34 806 34 1228 34 3678 Fragments arranged by size: 3678 1228 806 159 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2270 6002 2270 Size: 3732 2275 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 4068 5224 1790 Size: 2278 1156 2573 Fragments arranged by size: 2573 2278 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2067 2496 3020 Size: 416 53 83 399 24 650 429 524 Cuts at: 3020 3738 4696 442 Size: 718 958 1753 Fragments arranged by size: 1753 958 718 650 524 429 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2289 3213 4667 1239 Size: 1050 924 1454 2579 Fragments arranged by size: 2579 1454 1050 924 Bpu10I CC'TnA_GC Cuts at: 2529 2529 Size: 6007 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2443 4132 4997 5295 2443 Size: 1689 865 298 3155 Fragments arranged by size: 3155 1689 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 4670 882 Size: 617 706 2465 2219 Fragments arranged by size: 2465 2219 706 617 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3814 4196 4831 275 Size: 53 83 186 936 2281 382 635 1451 Fragments arranged by size: 2281 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5782 Fragments arranged by size: 5782 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2049 1186 Size: 863 5144 BseYI C'CCAG_C Cuts at: 5210 5706 5210 Size: 496 5511 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5434 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2056 4068 4153 5224 2056 Size: 2012 85 1071 2839 Fragments arranged by size: 2839 2012 1071 85 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 BsmBI CGTCTCn'nnnn_ Cuts at: 2097 2097 Size: 6007 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 3148 3326 4068 4153 5224 1391 Size: 399 266 1092 178 742 85 1071 2174 Fragments arranged by size: 2174 1092 1071 742 399 266 178 85 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2878 3295 3362 844 Size: 2034 417 67 3489 Fragments arranged by size: 3489 2034 417 67 BsrBI CCG'CTC Cuts at: 1394 1823 2197 3894 1394 Size: 429 374 1697 3507 Fragments arranged by size: 3507 1697 429 374 BsrDI GCAATG_nn' Cuts at: 65 1248 3265 4502 4676 65 Size: 1183 2017 1237 174 1396 Fragments arranged by size: 2017 1396 1237 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4651 4838 1700 Size: 110 2841 187 2869 Fragments arranged by size: 2869 2841 187 110 BsrGI T'GTAC_A Cuts at: 96 3262 96 Size: 3166 2841 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 3088 794 Size: 2294 3713 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstYI r'GATC_y Cuts at: 1972 2270 2612 2806 3198 3337 3704 4089 4106 Size: 298 342 194 392 139 367 385 17 Cuts at: 4106 5558 5644 5656 6002 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 392 385 367 346 342 298 194 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5455 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 3523 4317 4337 3523 Size: 794 20 5193 Fragments arranged by size: 5193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2959 5014 809 Size: 507 1643 2055 1802 Fragments arranged by size: 2055 1802 1643 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 3454 4343 Size: 54 1425 50 275 18 415 1209 889 Cuts at: 4343 5972 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1209 889 415 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2182 2943 3937 1353 Size: 829 761 994 3423 Fragments arranged by size: 3423 994 829 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2289 3191 3213 4070 4667 5454 5722 Size: 393 657 902 22 857 597 787 268 Cuts at: 5722 1239 Size: 1524 Fragments arranged by size: 1524 902 857 787 657 597 393 268 22 EcoO109I rG'GnC_Cy Cuts at: 3017 3058 3076 3017 Size: 41 18 5948 Fragments arranged by size: 5948 41 18 EcoRI G'AATT_C Cuts at: 2037 3316 3694 2037 Size: 1279 378 4350 Fragments arranged by size: 4350 1279 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2466 2466 Size: 6007 FspI TGC'GCA Cuts at: 2958 4513 2958 Size: 1555 4452 HaeII r_GCGC'y Cuts at: 1678 5154 1678 Size: 3476 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2597 2629 4648 4680 4722 Size: 32 135 32 1025 32 2019 32 42 Cuts at: 4722 4754 1373 Size: 32 2626 Fragments arranged by size: 2626 2019 1025 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3351 3597 669 Size: 988 281 1413 246 3079 Fragments arranged by size: 3079 1413 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 2894 3332 2894 Size: 438 5569 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3095 3113 5121 5305 1305 Size: 1790 18 2008 184 2007 Fragments arranged by size: 2008 2007 1790 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4185 Fragments arranged by size: 4185 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 2912 3965 4324 4483 518 Size: 648 907 839 1053 359 159 2042 Fragments arranged by size: 2042 1053 907 839 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3423 4100 5248 5493 1606 Size: 1817 677 1148 245 2120 Fragments arranged by size: 2120 1817 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5455 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 3365 4910 1132 Size: 97 2136 1545 2229 Fragments arranged by size: 2229 2136 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6007 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3017 3017 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 2208 3577 2208 Size: 1369 4638 PstI C_TGCA'G Cuts at: 830 2872 3057 3093 3359 830 Size: 2042 185 36 266 3478 Fragments arranged by size: 3478 2042 266 185 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SbfI CC_TGCA'GG Cuts at: 3057 3359 3057 Size: 302 5705 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2868 3053 3089 3355 4490 5171 5362 Size: 219 1823 185 36 266 1135 681 191 Cuts at: 5362 826 Size: 1471 Fragments arranged by size: 1823 1471 1135 681 266 219 191 185 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2458 4111 5012 5274 820 Size: 197 926 515 1653 901 262 1553 Fragments arranged by size: 1653 1553 926 901 515 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 2419 1517 Size: 902 5105 StyI C'CwwG_G Cuts at: 513 1065 3446 513 Size: 552 2381 3074 Fragments arranged by size: 3074 2381 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4034 4051 4204 4389 4834 325 Size: 1255 2454 17 153 185 445 1498 Fragments arranged by size: 2454 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3262 4253 5965 Size: 274 80 33 51 1443 1285 991 1712 Cuts at: 5965 96 Size: 138 Fragments arranged by size: 1712 1443 1285 991 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5967 5999 5967 Size: 32 5975 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3156 3156 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 2037 2254 4136 2037 Size: 217 1882 3908 Fragments arranged by size: 3908 1882 217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI