Restriction map for
pFN21AE1922
(Linear) (Six-base) MAPSORT of: KIEE1922.seq Check: 1222 from: 1 to: 1302
KIEE1922.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
Acc65I G'GTAC_C
Cuts at: 0 885 1302
Size: 885 417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1302
Size: 1186 116
AlwNI CAG_nnn'CTG
Cuts at: 0 1083 1302
Size: 1083 219
ApoI r'AATT_y
Cuts at: 0 32 587 843 1230 1302
Size: 32 555 256 387 72
Fragments arranged by size:
555 387 256 72 32
BamHI G'GATC_C
Cuts at: 0 607 1193 1302
Size: 607 586 109
Fragments arranged by size:
607 586 109
BanI G'GyrC_C
Cuts at: 0 361 885 1302
Size: 361 524 417
Fragments arranged by size:
524 417 361
BanII G_rGCy'C
Cuts at: 0 1143 1302
Size: 1143 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 1177 1302
Size: 1177 125
BbvCI CC'TCA_GC
Cuts at: 0 524 1302
Size: 524 778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 91 125 931 965 1302
Size: 91 34 806 34 337
Fragments arranged by size:
806 337 91 34 34
BglII A'GATC_T
Cuts at: 0 265 1302
Size: 265 1037
BmrI ACTGGGnnnn_n'
Cuts at: 0 62 491 1015 1302
Size: 62 429 524 287
Fragments arranged by size:
524 429 287 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1208 1302
Size: 284 924 94
Fragments arranged by size:
924 284 94
Bpu10I CC'TnA_GC
Cuts at: 0 524 1302
Size: 524 778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1302
Size: 438 864
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 1302
Size: 200 1102
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 44 1302
Size: 44 1258
BsiHKAI G_wGCw'C
Cuts at: 0 51 1302
Size: 51 1251
BsmBI CGTCTCn'nnnn_
Cuts at: 0 92 1302
Size: 92 1210
Bsp1286I G_dGCh'C
Cuts at: 0 51 1143 1302
Size: 51 1092 159
Fragments arranged by size:
1092 159 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 873 1302
Size: 873 429
BsrBI CCG'CTC
Cuts at: 0 192 1302
Size: 192 1110
BsrDI GCAATG_nn'
Cuts at: 0 1260 1302
Size: 1260 42
BsrGI T'GTAC_A
Cuts at: 0 1257 1302
Size: 1257 45
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1083 1302
Size: 1083 219
BstYI r'GATC_y
Cuts at: 0 265 607 801 1193 1302
Size: 265 342 194 392 109
Fragments arranged by size:
392 342 265 194 109
DrdI GACnn_nn'nnGTC
Cuts at: 0 954 1302
Size: 954 348
EaeI y'GGCC_r
Cuts at: 0 240 1302
Size: 240 1062
EarI CTCTTCn'nnn_
Cuts at: 0 177 938 1302
Size: 177 761 364
Fragments arranged by size:
761 364 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1186 1208 1302
Size: 284 902 22 94
Fragments arranged by size:
902 284 94 22
EcoO109I rG'GnC_Cy
Cuts at: 0 1012 1053 1071 1302
Size: 1012 41 18 231
Fragments arranged by size:
1012 231 41 18
EcoRI G'AATT_C
Cuts at: 0 32 1302
Size: 32 1270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 461 1302
Size: 461 841
FspI TGC'GCA
Cuts at: 0 953 1302
Size: 953 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 1302
Size: 592 32 678
Fragments arranged by size:
678 592 32
KpnI G_GTAC'C
Cuts at: 0 889 1302
Size: 889 413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1108 1302
Size: 1090 18 194
Fragments arranged by size:
1090 194 18
MslI CAynn'nnrTG
Cuts at: 0 68 907 1302
Size: 68 839 395
Fragments arranged by size:
839 395 68
PpuMI rG'GwC_Cy
Cuts at: 0 1012 1302
Size: 1012 290
PsiI TTA'TAA
Cuts at: 0 203 1302
Size: 203 1099
PstI C_TGCA'G
Cuts at: 0 867 1052 1088 1302
Size: 867 185 36 214
Fragments arranged by size:
867 214 185 36
SbfI CC_TGCA'GG
Cuts at: 0 1052 1302
Size: 1052 250
SfcI C'TryA_G
Cuts at: 0 863 1048 1084 1302
Size: 863 185 36 218
Fragments arranged by size:
863 218 185 36
SmlI C'TyrA_G
Cuts at: 0 453 1302
Size: 453 849
StuI AGG'CCT
Cuts at: 0 414 1302
Size: 414 888
TatI w'GTAC_w
Cuts at: 0 1257 1302
Size: 1257 45
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1151 1302
Size: 1151 151
XmnI GAAnn'nnTTC
Cuts at: 0 32 249 1302
Size: 32 217 1053
Fragments arranged by size:
1053 217 32
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI BamHI BanI BanII BbsI
BbvCI BcgI BglII BmrI BpmI Bpu10I BpuEI BsaI
BseRI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstAPI BstYI DrdI EaeI EarI Eco57MI EcoO109I EcoRI
FalI FspI Hin4I KpnI MmeI MslI PpuMI PsiI
PstI SbfI SfcI SmlI StuI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI
BspEI BspHI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRV FseI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI ZraI