################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AccI AccIII AclI AflIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AviII BciVI BglII BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI HgaI HindIII HpaI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1861 GGTACC G^GTACC AcyI 2738 GGCGCC G[GA]^CG[CT]C AflII 153 CTTAAG C^TTAAG Alw44I 1810 GTGCAC G^TGCAC AocI 477 CCTGAGG CC^T[ACGT]AGG ApaLI 1810 GTGCAC G^TGCAC Asp700I 2199 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1861 GGTACC G^GTACC BbeI 2738 GGCGCC GGCGC^C BbrPI 1951 CACGTG CAC^GTG BcgI 294 CGAAGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 153 CTTAAG C^TTAAG BsaBI 1689 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2738 GGCGCC G[GA]^CG[CT]C BsaI 771 GGTCTC GGTCTC(1/5) BsiEI 846 CGACCG CG[GA][CT]^CG BsrBI 553 GAGCGG CCGCTC(-3/-3) BstXI 2268 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 477 CCTGAGG CC^T[ACGT]AGG DrdI 1735 GACGGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1139 CCGCGG C^C[GA][CT]GG Eco81I 477 CCTGAGG CC^T[ACGT]AGG EcoRI 2017 GAATTC G^AATTC Hin1I 2738 GGCGCC G[GA]^CG[CT]C HincII 2516 GTCAAC GT[CT]^[GA]AC HindII 2516 GTCAAC GT[CT]^[GA]AC KasI 2738 GGCGCC G^GCGCC KpnI 1861 GGTACC GGTAC^C KspI 1139 CCGCGG CCGC^GG MamI 1689 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 1206 CACGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 2617 GCCGGC GCC^GGC NarI 2738 GGCGCC GG^CGCC NgoMIV 2617 GCCGGC G^CCGGC PmaCI 1951 CACGTG CAC^GTG PmlI 1951 CACGTG CAC^GTG PshAI 1648 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 1139 CCGCGG CCGC^GG SfiI 2170 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2738 GGCGCC GGC^GCC XcmI 2003 CCAACCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2199 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 1140 CGCG CG^CG 1927 CGCG AcsI 1348 AAATTC [GA]^AATT[CT] 2017 GAATTC ApaI 170 GGGCCC GGGCC^C 2303 GGGCCC ApoI 1348 AAATTC [GA]^AATT[CT] 2017 GAATTC AvrII 822 CCTAGG C^CTAGG 2364 CCTAGG BalI 2277 TGGCCA TGG^CCA 2607 TGGCCA BamHI 2625 GGATCC G^GATCC 2802 GGATCC BclI 1169 TGATCA T^GATCA 1508 TGATCA BlnI 822 CCTAGG C^CTAGG 2364 CCTAGG BlpI 550 GCTGAGC GC^T[ACGT]AGC 1921 GCTGAGC Bpu1102I 550 GCTGAGC GC^T[ACGT]AGC 1921 GCTGAGC BsaAI 1273 TACGTG [CT]AC^GT[GA] 1951 CACGTG BsmI 1834 GAATGC GAATGC(1/-1) 2069 GCATTC BspMI 2135 ACCTGC ACCTGC(4/8) 2238 GCAGGT BsrDI 1851 CATTGC GCAATG(2/0) 2668 CATTGC BssSI 877 CACGAG CACGAG(-5/-1) 958 CACGAG BstUI 1140 CGCG CG^CG 1927 CGCG CelII 550 GCTGAGC GC^T[ACGT]AGC 1921 GCTGAGC FbaI 1169 TGATCA T^GATCA 1508 TGATCA HaeII 473 AGCGCC [GA]GCGC^[CT] 2738 GGCGCC MluNI 2277 TGGCCA TGG^CCA 2607 TGGCCA MscI 2277 TGGCCA TGG^CCA 2607 TGGCCA MseI 154 TTAA T^TAA 1376 TTAA MvnI 1140 CGCG CG^CG 1927 CGCG PpuMI 371 GGGACCT [GA]G^G[AT]CC[CT] 2858 AGGACCC SapI 741 GAAGAGC GCTCTTC(1/4) 1500 GAAGAGC SmaI 1040 CCCGGG CCC^GGG 1562 CCCGGG SmlI 153 CTTAAG C^T[CT][GA]AG 457 CTCAAG StuI 316 AGGCCT AGG^CCT 2270 AGGCCT Tru9I 154 TTAA T^TAA 1376 TTAA XmaCI 1040 CCCGGG C^CCGGG 1562 CCCGGG XmaI 1040 CCCGGG C^CCGGG 1562 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SphI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII