Restriction map for
pFN21AE2204
(Circular) (Six-base) MAPSORT of: pFN21AE2204.seq Check: 7586 from: 1 to: 7615
pFN21AE2204 7615 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4234 4234
Size: 7615
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5425 278
Size: 53 83 186 936 3889 2468
Fragments arranged by size:
3889 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4958 1937
Size: 3021 4594
Acc65I G'GTAC_C
Cuts at: 3866 4936 3866
Size: 1070 6545
AclI AA'CG_TT
Cuts at: 1563 5742 6115 1563
Size: 4179 373 3063
Fragments arranged by size:
4179 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3347 3449 3651 3809 4932 5678 7062 7330
Size: 1715 102 202 158 1123 746 1384 268
Cuts at: 7330 1632
Size: 1917
Fragments arranged by size:
1917 1715 1384 1123 746 268 202 158
102
AflII C'TTAA_G
Cuts at: 820 1017 2158 820
Size: 197 1141 6277
Fragments arranged by size:
6277 1141 197
AflIII A'CryG_T
Cuts at: 1203 6514 7491 1203
Size: 5311 977 1327
Fragments arranged by size:
5311 1327 977
AgeI A'CCGG_T
Cuts at: 6446 6446
Size: 7615
AhdI GACnn_n'nnGTC
Cuts at: 6344 6344
Size: 7615
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2549 3173 1184
Size: 1365 624 5626
Fragments arranged by size:
5626 1365 624
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 7583
AlwNI CAG_nnn'CTG
Cuts at: 2443 3025 3118 3271 3337 3406 3613 3647 3901
Size: 582 93 153 66 69 207 34 254
Cuts at: 3901 3904 4018 4249 5358 6930 2443
Size: 3 114 231 1109 1572 3128
Fragments arranged by size:
3128 1572 1109 582 254 231 207 153
114 93 69 66 34 3
ApaI G_GGCC'C
Cuts at: 2179 4312 2179
Size: 2133 5482
ApaLI G'TGCA_C
Cuts at: 3815 5672 6828 3815
Size: 1857 1156 4602
Fragments arranged by size:
4602 1857 1156
ApoI r'AATT_y
Cuts at: 1493 3353 4022 4924 5150 5302 7411 1493
Size: 1860 669 902 226 152 2109 1697
Fragments arranged by size:
2109 1860 1697 902 669 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6169 160
Size: 872 5137 1606
Fragments arranged by size:
5137 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7615
AvaI C'yCGr_G
Cuts at: 1870 1943 2317 3045 3567 4940 1870
Size: 73 374 728 522 1373 4545
Fragments arranged by size:
4545 1373 728 522 374 73
AvrII C'CTAG_G
Cuts at: 2827 4369 2827
Size: 1542 6073
BamHI G'GATC_C
Cuts at: 4630 4807 4945 5312 4630
Size: 177 138 367 6933
Fragments arranged by size:
6933 367 177 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2312 3866 4475 4549
Size: 325 217 627 243 282 1554 609 74
Cuts at: 4549 4743 4936 6391 618
Size: 194 193 1455 1842
Fragments arranged by size:
1842 1554 1455 627 609 325 282 243
217 194 193 74
BanII G_rGCy'C
Cuts at: 1391 2179 2753 4312 4640 4934 1391
Size: 788 574 1559 328 294 4072
Fragments arranged by size:
4072 1559 788 574 328 294
BbeI G_GCGC'C
Cuts at: 4747 4747
Size: 7615
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2082 2280 2820 3437 928
Size: 971 183 198 540 617 5106
Fragments arranged by size:
5106 971 617 540 198 183
BbvCI CC'TCA_GC
Cuts at: 2522 2901 4460 2522
Size: 379 1559 5677
Fragments arranged by size:
5677 1559 379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 5806 5840 1903
Size: 34 351 34 3484 34 3678
Fragments arranged by size:
3678 3484 351 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5507 6717 5507
Size: 1210 6405
BclI T'GATC_A
Cuts at: 1548 3174 3513 1548
Size: 1626 339 5650
Fragments arranged by size:
5650 1626 339
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2757 3765 4182
Size: 107 122 71 631 605 1085 1008 417
Cuts at: 4182 6226 136
Size: 2044 1525
Fragments arranged by size:
2044 1525 1085 1008 631 605 417 122
107 71
BglII A'GATC_T
Cuts at: 7610 7610
Size: 7615
BlpI GC'TnA_GC
Cuts at: 2556 3927 5032 2556
Size: 1371 1105 5139
Fragments arranged by size:
5139 1371 1105
Bme1580I G_kGCm'C
Cuts at: 1790 2179 3819 4312 4753 5676 6832 1790
Size: 389 1640 493 441 923 1156 2573
Fragments arranged by size:
2573 1640 1156 923 493 441 389
BmgBI CAC'GTC
Cuts at: 1318 2818 1318
Size: 1500 6115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2157 2719 4171
Size: 416 53 83 399 24 740 562 1452
Cuts at: 4171 5346 6304 442
Size: 1175 958 1753
Fragments arranged by size:
1753 1452 1175 958 740 562 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7615
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2528
Size: 7615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2349 2585 2594 2687 3368 3951 4125 4793
Size: 1110 236 9 93 681 583 174 668
Cuts at: 4793 6275 1239
Size: 1482 2579
Fragments arranged by size:
2579 1482 1110 681 668 583 236 174
93 9
Bpu10I CC'TnA_GC
Cuts at: 2522 2901 3755 4460 2522
Size: 379 854 705 5677
Fragments arranged by size:
5677 854 705 379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 5740 6605 6903 2447
Size: 3293 865 298 3159
Fragments arranged by size:
3293 3159 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2782 6278 882
Size: 617 1283 3496 2219
Fragments arranged by size:
3496 2219 1283 617
BsaAI yAC'GTr
Cuts at: 493 1204 3280 3958 5406 493
Size: 711 2076 678 1448 2702
Fragments arranged by size:
2702 2076 1448 711 678
BsaBI GATnn'nnATC
Cuts at: 3698 7609 3698
Size: 3911 3704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4744 5422 5804 6439
Size: 53 83 186 936 3211 678 382 635
Cuts at: 6439 275
Size: 1451
Fragments arranged by size:
3211 1451 936 678 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6048 6446 6720 6867 7278 6048
Size: 398 274 147 411 6385
Fragments arranged by size:
6385 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4098 4128 4491 4521 4766
Size: 30 165 30 2695 30 363 30 245
Cuts at: 4766 4796 1178
Size: 30 3997
Fragments arranged by size:
3997 2695 363 245 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 2600 2603 2636 2918 2921 2935 2938
Size: 1318 96 3 33 282 3 14 3
Cuts at: 2938 4307 4849 1186
Size: 1369 542 3952
Fragments arranged by size:
3952 1369 1318 542 282 96 33 14
3 3 3
BseYI C'CCAG_C
Cuts at: 2715 3090 3243 3264 3587 4180 6818 7314 2715
Size: 375 153 21 323 593 2638 496 3016
Fragments arranged by size:
3016 2638 593 496 375 323 153 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2477 2858 3509 4704 1163
Size: 573 642 99 381 651 1195 4074
Fragments arranged by size:
4074 1195 651 642 573 381 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 5065 5826 6854 1540
Size: 275 186 853 2211 761 1028 2301
Fragments arranged by size:
2301 2211 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3785 3819 4579 5676 5761 6832 3785
Size: 34 760 1097 85 1071 4568
Fragments arranged by size:
4568 1097 1071 760 85 34
BsmI GAATG_Cn'
Cuts at: 3845 4074 5126 5219 3845
Size: 229 1052 93 6241
Fragments arranged by size:
6241 1052 229 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2753 3785 3819 4312 4579 4640
Size: 399 389 574 1032 34 493 267 61
Cuts at: 4640 4753 4934 5676 5761 6832 1391
Size: 113 181 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 742 574 493 399 389
267 181 113 85 61 34
BspEI T'CCGG_A
Cuts at: 7278 7278
Size: 7615
BspHI T'CATG_A
Cuts at: 5504 7234 5504
Size: 1730 5885
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4149 4234 4970 844
Size: 3305 85 736 3489
Fragments arranged by size:
3489 3305 736 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2560 5502 1394
Size: 429 737 2942 3507
Fragments arranged by size:
3507 2942 737 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3855 4672 6110 6284 65
Size: 1183 2607 817 1438 174 1396
Fragments arranged by size:
2607 1438 1396 1183 817 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2335 3877 4374 4622 6259 6446 1700
Size: 110 525 1542 497 248 1637 187 2869
Fragments arranged by size:
2869 1637 1542 525 497 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7615
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7615
BssSI C'ACGA_G
Cuts at: 2882 2963 5675 6687 2882
Size: 81 2712 1012 3810
Fragments arranged by size:
3810 2712 1012 81
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2443 2455 3604 3613 3647 3901 4249 794
Size: 1649 12 1149 9 34 254 348 4160
Fragments arranged by size:
4160 1649 1149 348 254 34 12 9
BstBI TT'CG_AA
Cuts at: 6419 6419
Size: 7615
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7615
BstXI CCAn_nnnn'nTGG
Cuts at: 4280 4280
Size: 7615
BstYI r'GATC_y
Cuts at: 1972 3333 4002 4630 4807 4945 5312 5697 5714
Size: 1361 669 628 177 138 367 385 17
Cuts at: 5714 7166 7252 7264 7610 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1361 669 628 385 367 346
177 138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2483 2483
Size: 7615
BtgI C'CryG_G
Cuts at: 513 1065 2004 3144 513
Size: 552 939 1140 4984
Fragments arranged by size:
4984 1140 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6876
Fragments arranged by size:
6876 683 56
BtsI GCAGTG_nn'
Cuts at: 3561 3855 4734 5131 5925 5945 3561
Size: 294 879 397 794 20 5231
Fragments arranged by size:
5231 879 794 397 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7580
DraI TTT'AAA
Cuts at: 4919 4919
Size: 7615
DraIII CAC_nnn'GTG
Cuts at: 2455 3226 4237 2455
Size: 771 1011 5833
Fragments arranged by size:
5833 1011 771
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3746 6622 809
Size: 507 2430 2876 1802
Fragments arranged by size:
2876 2430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3129 3729 4282
Size: 54 1425 50 275 18 1299 600 553
Cuts at: 4282 4469 4612 5062 5951 7580 8
Size: 187 143 450 889 1629 43
Fragments arranged by size:
1629 1425 1299 889 600 553 450 275
187 143 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5062 1537
Size: 275 3250 4090
Fragments arranged by size:
4090 3250 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2364 2741 3455 3500 4718 5545 1353
Size: 861 150 377 714 45 1218 827 3423
Fragments arranged by size:
3423 1218 861 827 714 377 150 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2414 2507 6202 6576 6722 387
Size: 1064 138 825 93 3695 374 146 1280
Fragments arranged by size:
3695 1280 1064 825 374 146 138 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2585 2594 2687 3347 3368 3449
Size: 393 717 236 9 93 660 21 81
Cuts at: 3449 3651 3809 3951 4125 4793 4932 5678 6275
Size: 202 158 142 174 668 139 746 597
Cuts at: 6275 7062 7330 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 746 717 668 660 597 393
268 236 202 174 158 142 139 93
81 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2486 2999 3035 3482 4145 2486
Size: 513 36 447 663 5956
Fragments arranged by size:
5956 663 513 447 36
EcoO109I rG'GnC_Cy
Cuts at: 2175 2176 2377 2992 4308 4309 4864 2175
Size: 1 201 615 1316 1 555 4926
Fragments arranged by size:
4926 1316 615 555 201 1 1
EcoRI G'AATT_C
Cuts at: 4022 4924 5302 4022
Size: 902 378 6335
Fragments arranged by size:
6335 902 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 7615
FspI TGC'GCA
Cuts at: 6121 6121
Size: 7615
HaeII r_GCGC'y
Cuts at: 1678 2482 4747 6762 1678
Size: 804 2265 2015 2531
Fragments arranged by size:
2531 2265 2015 804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3619 3651 4098 4130 6256
Size: 32 135 32 2047 32 447 32 2126
Cuts at: 6256 6288 6330 6362 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2126 2047 447 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4523 4959 5205 669
Size: 988 281 2585 436 246 3079
Fragments arranged by size:
3079 2585 988 436 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7615
HpaI GTT'AAC
Cuts at: 5205 5205
Size: 7615
KasI G'GCGC_C
Cuts at: 4743 4743
Size: 7615
KpnI G_GTAC'C
Cuts at: 3870 4940 3870
Size: 1070 6545
MfeI C'AATT_G
Cuts at: 5214 5214
Size: 7615
MluI A'CGCG_T
Cuts at: 7491 7491
Size: 7615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2162 2684 4032 4309 6729 6913 1305
Size: 857 522 1348 277 2420 184 2007
Fragments arranged by size:
2420 2007 1348 857 522 277 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4284 4614 10
Size: 54 1425 343 2452 330 3011
Fragments arranged by size:
3011 2452 1425 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3215 5573 5932 6091 518
Size: 648 2049 2358 359 159 2042
Fragments arranged by size:
2358 2049 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2443 2572 2719 2856 3022 3094 3146 3237
Size: 837 129 147 137 166 72 52 91
Cuts at: 3237 3268 3812 3901 3999 4018 4771 5031 5708
Size: 31 544 89 98 19 753 260 677
Cuts at: 5708 6856 7101 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 837 753 677 544 260 245
166 147 137 129 98 91 89 72
52 31 19
NaeI GCC'GGC
Cuts at: 4624 4624
Size: 7615
NarI GG'CG_CC
Cuts at: 4744 4744
Size: 7615
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6124
Fragments arranged by size:
6124 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7615
NgoMIV G'CCGG_C
Cuts at: 4622 4622
Size: 7615
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7615
NotI GC'GGCC_GC
Cuts at: 5062 5062
Size: 7615
NspI r_CATG'y
Cuts at: 1132 1229 2869 3128 3220 4399 4973 6518 1132
Size: 97 1640 259 92 1179 574 1545 2229
Fragments arranged by size:
2229 1640 1545 1179 574 259 97 92
PasI CC'CwG_GG
Cuts at: 1348 4482 1348
Size: 3134 4481
PciI A'CATG_T
Cuts at: 6514 6514
Size: 7615
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3097 4018 4740 1108
Size: 1989 921 722 3983
Fragments arranged by size:
3983 1989 921 722
PfoI T'CCnGG_A
Cuts at: 2070 2982 4102 4212 4579 2070
Size: 912 1120 110 367 5106
Fragments arranged by size:
5106 1120 912 367 110
PmeI GTTT'AAAC
Cuts at: 4919 4919
Size: 7615
PmlI CAC'GTG
Cuts at: 3958 3958
Size: 7615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5684 5716 7197 7229 5684
Size: 32 1481 32 6070
Fragments arranged by size:
6070 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 4864 2377
Size: 2487 5128
PshAI GACnn'nnGTC
Cuts at: 1613 3657 1613
Size: 2044 5571
PsiI TTA'TAA
Cuts at: 5185 5185
Size: 7615
PspOMI G'GGCC_C
Cuts at: 2175 4308 2175
Size: 2133 5482
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 7583
PstI C_TGCA'G
Cuts at: 830 2311 2439 2448 3088 3162 3276 3609 3618
Size: 1481 128 9 640 74 114 333 9
Cuts at: 3618 3652 3998 4423 4431 4967 830
Size: 34 346 425 8 536 3478
Fragments arranged by size:
3478 1481 640 536 425 346 333 128
114 74 34 9 9 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7615
PvuII CAG'CTG
Cuts at: 2443 2572 2719 3022 3094 3268 3812 3901 2443
Size: 129 147 303 72 174 544 89 6157
Fragments arranged by size:
6157 544 303 174 147 129 89 72
SacII CC_GC'GG
Cuts at: 3147 3147
Size: 7615
SalI G'TCGA_C
Cuts at: 1936 4957 1936
Size: 3021 4594
SapI GCTCTTCn'nnn_
Cuts at: 2741 3500 2741
Size: 759 6856
SbfI CC_TGCA'GG
Cuts at: 4967 4967
Size: 7615
ScaI AGT'ACT
Cuts at: 5863 7575 5863
Size: 1712 5903
SexAI A'CCwGG_T
Cuts at: 2379 3165 4233 2379
Size: 786 1068 5761
Fragments arranged by size:
5761 1068 786
SfcI C'TryA_G
Cuts at: 826 1045 2307 2435 2444 2698 3084 3158 3272
Size: 219 1262 128 9 254 386 74 114
Cuts at: 3272 3605 3614 3648 3994 4419 4427 4963 6098
Size: 333 9 34 346 425 8 536 1135
Cuts at: 6098 6779 6970 826
Size: 681 191 1471
Fragments arranged by size:
1471 1262 1135 681 536 425 386 346
333 254 219 191 128 114 74 34
9 9 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 4182 4182
Size: 7615
SfoI GGC'GCC
Cuts at: 4745 4745
Size: 7615
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7615
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7615
SmaI CCC'GGG
Cuts at: 1872 3047 3569 4942 1872
Size: 1175 522 1373 4545
Fragments arranged by size:
4545 1373 1175 522
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2158 2462 5719 6620 6882 820
Size: 197 926 215 304 3257 901 262 1553
Fragments arranged by size:
3257 1553 926 901 304 262 215 197
SnaBI TAC'GTA
Cuts at: 493 5406 493
Size: 4913 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7615
SphI G_CATG'C
Cuts at: 1132 3128 3220 4399 4973 1132
Size: 1996 92 1179 574 3774
Fragments arranged by size:
3774 1996 1179 574 92
SrfI GCCC'GGGC
Cuts at: 3047 3047
Size: 7615
SspI AAT'ATT
Cuts at: 5 52 5539 5
Size: 47 5487 2081
Fragments arranged by size:
5487 2081 47
StuI AGG'CCT
Cuts at: 1517 2323 4277 1517
Size: 806 1954 4855
Fragments arranged by size:
4855 1954 806
StyI C'CwwG_G
Cuts at: 513 1065 2004 2782 2827 3031 3703 3763 4369
Size: 552 939 778 45 204 672 60 606
Cuts at: 4369 5054 513
Size: 685 3074
Fragments arranged by size:
3074 939 778 685 672 606 552 204
60 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2403 5642 5659 5812 5997 6442 325
Size: 1255 823 3239 17 153 185 445 1498
Fragments arranged by size:
3239 1498 1255 823 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5861 7573 96
Size: 274 80 33 51 1443 3884 1712 138
Fragments arranged by size:
3884 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4491 4523 7575 7607 4491
Size: 32 3052 32 4499
Fragments arranged by size:
4499 3052 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7615
XbaI T'CTAG_A
Cuts at: 4951 4951
Size: 7615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4015 4015
Size: 7615
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7615
XmaI C'CCGG_G
Cuts at: 1870 3045 3567 4940 1870
Size: 1175 522 1373 4545
Fragments arranged by size:
4545 1373 1175 522
XmnI GAAnn'nnTTC
Cuts at: 4208 5744 4208
Size: 1536 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5423 276
Size: 53 83 186 936 3889 2468
Fragments arranged by size:
3889 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PspXI RsrII
SacI SanDI SwaI