(Linear) (Six-base) MAPSORT of: KIEE2204.seq Check: 224 from: 1 to: 2910 KIEE2204.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2229 2910 Size: 2229 681 Acc65I G'GTAC_C Cuts at: 0 1861 2910 Size: 1861 1049 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1342 1444 1646 1804 2910 Size: 1342 102 202 158 1106 Fragments arranged by size: 1342 1106 202 158 102 AflII C'TTAA_G Cuts at: 0 153 2910 Size: 153 2757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 544 1168 2910 Size: 544 624 1742 Fragments arranged by size: 1742 624 544 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2008 2040 2910 Size: 2008 32 870 Fragments arranged by size: 2008 870 32 AlwNI CAG_nnn'CTG Cuts at: 0 438 1020 1113 1266 1332 1401 1608 1642 Size: 438 582 93 153 66 69 207 34 Cuts at: 1642 1896 1899 2013 2244 2910 Size: 254 3 114 231 666 Fragments arranged by size: 666 582 438 254 231 207 153 114 93 69 66 34 3 ApaI G_GGCC'C Cuts at: 0 174 2307 2910 Size: 174 2133 603 Fragments arranged by size: 2133 603 174 ApaLI G'TGCA_C Cuts at: 0 1810 2910 Size: 1810 1100 ApoI r'AATT_y Cuts at: 0 1348 2017 2910 Size: 1348 669 893 Fragments arranged by size: 1348 893 669 AvaI C'yCGr_G Cuts at: 0 312 1040 1562 2910 Size: 312 728 522 1348 Fragments arranged by size: 1348 728 522 312 AvrII C'CTAG_G Cuts at: 0 822 2364 2910 Size: 822 1542 546 Fragments arranged by size: 1542 822 546 BamHI G'GATC_C Cuts at: 0 2625 2802 2910 Size: 2625 177 108 Fragments arranged by size: 2625 177 108 BanI G'GyrC_C Cuts at: 0 25 307 1861 2470 2544 2738 2910 Size: 25 282 1554 609 74 194 172 Fragments arranged by size: 1554 609 282 194 172 74 25 BanII G_rGCy'C Cuts at: 0 174 748 2307 2635 2910 Size: 174 574 1559 328 275 Fragments arranged by size: 1559 574 328 275 174 BbeI G_GCGC'C Cuts at: 0 2742 2910 Size: 2742 168 BbsI GAAGACnn'nnnn_ Cuts at: 0 77 275 815 1432 2910 Size: 77 198 540 617 1478 Fragments arranged by size: 1478 617 540 198 77 BbvCI CC'TCA_GC Cuts at: 0 517 896 2455 2910 Size: 517 379 1559 455 Fragments arranged by size: 1559 517 455 379 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 283 317 2910 Size: 283 34 2593 Fragments arranged by size: 2593 283 34 BclI T'GATC_A Cuts at: 0 1169 1508 2910 Size: 1169 339 1402 Fragments arranged by size: 1402 1169 339 BglI GCCn_nnn'nGGC Cuts at: 0 752 1760 2177 2910 Size: 752 1008 417 733 Fragments arranged by size: 1008 752 733 417 BlpI GC'TnA_GC Cuts at: 0 551 1922 2910 Size: 551 1371 988 Fragments arranged by size: 1371 988 551 Bme1580I G_kGCm'C Cuts at: 0 174 1814 2307 2748 2910 Size: 174 1640 493 441 162 Fragments arranged by size: 1640 493 441 174 162 BmgBI CAC'GTC Cuts at: 0 813 2910 Size: 813 2097 BmrI ACTGGGnnnn_n' Cuts at: 0 152 714 2166 2910 Size: 152 562 1452 744 Fragments arranged by size: 1452 744 562 152 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 523 2910 Size: 523 2387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 580 589 682 1363 1946 2120 2788 Size: 344 236 9 93 681 583 174 668 Cuts at: 2788 2910 Size: 122 Fragments arranged by size: 681 668 583 344 236 174 122 93 9 Bpu10I CC'TnA_GC Cuts at: 0 517 896 1750 2455 2910 Size: 517 379 854 705 455 Fragments arranged by size: 854 705 517 455 379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 2910 Size: 442 2468 BsaI GGTCTCn'nnnn_ Cuts at: 0 777 2910 Size: 777 2133 BsaAI yAC'GTr Cuts at: 0 1275 1953 2910 Size: 1275 678 957 Fragments arranged by size: 1275 957 678 BsaBI GATnn'nnATC Cuts at: 0 1693 2910 Size: 1693 1217 BsaHI Gr'CG_yC Cuts at: 0 2739 2910 Size: 2739 171 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2093 2123 2486 2516 2761 2791 2910 Size: 2093 30 363 30 245 30 119 Fragments arranged by size: 2093 363 245 119 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 499 595 598 631 913 916 930 933 Size: 499 96 3 33 282 3 14 3 Cuts at: 933 2302 2844 2910 Size: 1369 542 66 Fragments arranged by size: 1369 542 499 282 96 66 33 14 3 3 3 BseYI C'CCAG_C Cuts at: 0 710 1085 1238 1259 1582 2175 2910 Size: 710 375 153 21 323 593 735 Fragments arranged by size: 735 710 593 375 323 153 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 472 853 1504 2699 2910 Size: 373 99 381 651 1195 211 Fragments arranged by size: 1195 651 381 373 211 99 BsiEI CG_ry'CG Cuts at: 0 849 2910 Size: 849 2061 BsiHKAI G_wGCw'C Cuts at: 0 1780 1814 2574 2910 Size: 1780 34 760 336 Fragments arranged by size: 1780 760 336 34 BsmI GAATG_Cn' Cuts at: 0 1840 2069 2910 Size: 1840 229 841 Fragments arranged by size: 1840 841 229 Bsp1286I G_dGCh'C Cuts at: 0 174 748 1780 1814 2307 2574 2635 2748 Size: 174 574 1032 34 493 267 61 113 Cuts at: 2748 2910 Size: 162 Fragments arranged by size: 1032 574 493 267 174 162 113 61 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2144 2229 2910 Size: 2144 85 681 Fragments arranged by size: 2144 681 85 BsrBI CCG'CTC Cuts at: 0 555 2910 Size: 555 2355 BsrDI GCAATG_nn' Cuts at: 0 1850 2667 2910 Size: 1850 817 243 Fragments arranged by size: 1850 817 243 BsrFI r'CCGG_y Cuts at: 0 330 1872 2369 2617 2910 Size: 330 1542 497 248 293 Fragments arranged by size: 1542 497 330 293 248 BssSI C'ACGA_G Cuts at: 0 877 958 2910 Size: 877 81 1952 Fragments arranged by size: 1952 877 81 BstAPI GCAn_nnn'nTGC Cuts at: 0 438 450 1599 1608 1642 1896 2244 2910 Size: 438 12 1149 9 34 254 348 666 Fragments arranged by size: 1149 666 438 348 254 34 12 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 2275 2910 Size: 2275 635 BstYI r'GATC_y Cuts at: 0 1328 1997 2625 2802 2910 Size: 1328 669 628 177 108 Fragments arranged by size: 1328 669 628 177 108 Bsu36I CC'TnA_GG Cuts at: 0 478 2910 Size: 478 2432 BtgI C'CryG_G Cuts at: 0 1139 2910 Size: 1139 1771 BtsI GCAGTG_nn' Cuts at: 0 1556 1850 2729 2910 Size: 1556 294 879 181 Fragments arranged by size: 1556 879 294 181 DraIII CAC_nnn'GTG Cuts at: 0 450 1221 2232 2910 Size: 450 771 1011 678 Fragments arranged by size: 1011 771 678 450 DrdI GACnn_nn'nnGTC Cuts at: 0 1741 2910 Size: 1741 1169 EaeI y'GGCC_r Cuts at: 0 1124 1724 2277 2464 2607 2910 Size: 1124 600 553 187 143 303 Fragments arranged by size: 1124 600 553 303 187 143 EarI CTCTTCn'nnn_ Cuts at: 0 209 359 736 1450 1495 2713 2910 Size: 209 150 377 714 45 1218 197 Fragments arranged by size: 1218 714 377 209 197 150 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 409 502 2910 Size: 409 93 2408 Fragments arranged by size: 2408 409 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 580 589 682 1342 1363 1444 1646 Size: 344 236 9 93 660 21 81 202 Cuts at: 1646 1804 1946 2120 2788 2910 Size: 158 142 174 668 122 Fragments arranged by size: 668 660 344 236 202 174 158 142 122 93 81 21 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 481 994 1030 1477 2140 2910 Size: 481 513 36 447 663 770 Fragments arranged by size: 770 663 513 481 447 36 EcoO109I rG'GnC_Cy Cuts at: 0 170 171 372 987 2303 2304 2859 2910 Size: 170 1 201 615 1316 1 555 51 Fragments arranged by size: 1316 615 555 201 170 51 1 1 EcoRI G'AATT_C Cuts at: 0 2017 2910 Size: 2017 893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 765 2910 Size: 765 2145 HaeII r_GCGC'y Cuts at: 0 477 2742 2910 Size: 477 2265 168 Fragments arranged by size: 2265 477 168 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1614 1646 2093 2125 2910 Size: 1614 32 447 32 785 Fragments arranged by size: 1614 785 447 32 32 HincII GTy'rAC Cuts at: 0 2518 2910 Size: 2518 392 KasI G'GCGC_C Cuts at: 0 2738 2910 Size: 2738 172 KpnI G_GTAC'C Cuts at: 0 1865 2910 Size: 1865 1045 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 157 679 2027 2304 2910 Size: 157 522 1348 277 606 Fragments arranged by size: 1348 606 522 277 157 MscI TGG'CCA Cuts at: 0 2279 2609 2910 Size: 2279 330 301 Fragments arranged by size: 2279 330 301 MslI CAynn'nnrTG Cuts at: 0 1210 2910 Size: 1210 1700 MspA1I CmG'CkG Cuts at: 0 438 567 714 851 1017 1089 1141 1232 Size: 438 129 147 137 166 72 52 91 Cuts at: 1232 1263 1807 1896 1994 2013 2766 2910 Size: 31 544 89 98 19 753 144 Fragments arranged by size: 753 544 438 166 147 144 137 129 98 91 89 72 52 31 19 NaeI GCC'GGC Cuts at: 0 2619 2910 Size: 2619 291 NarI GG'CG_CC Cuts at: 0 2739 2910 Size: 2739 171 NgoMIV G'CCGG_C Cuts at: 0 2617 2910 Size: 2617 293 NspI r_CATG'y Cuts at: 0 864 1123 1215 2394 2910 Size: 864 259 92 1179 516 Fragments arranged by size: 1179 864 516 259 92 PasI CC'CwG_GG Cuts at: 0 2477 2910 Size: 2477 433 PflMI CCAn_nnn'nTGG Cuts at: 0 1092 2013 2735 2910 Size: 1092 921 722 175 Fragments arranged by size: 1092 921 722 175 PfoI T'CCnGG_A Cuts at: 0 65 977 2097 2207 2574 2910 Size: 65 912 1120 110 367 336 Fragments arranged by size: 1120 912 367 336 110 65 PmlI CAC'GTG Cuts at: 0 1953 2910 Size: 1953 957 PpuMI rG'GwC_Cy Cuts at: 0 372 2859 2910 Size: 372 2487 51 Fragments arranged by size: 2487 372 51 PshAI GACnn'nnGTC Cuts at: 0 1652 2910 Size: 1652 1258 PspOMI G'GGCC_C Cuts at: 0 170 2303 2910 Size: 170 2133 607 Fragments arranged by size: 2133 607 170 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1300 1332 2910 Size: 1300 32 1578 Fragments arranged by size: 1578 1300 32 PstI C_TGCA'G Cuts at: 0 306 434 443 1083 1157 1271 1604 1613 Size: 306 128 9 640 74 114 333 9 Cuts at: 1613 1647 1993 2418 2426 2910 Size: 34 346 425 8 484 Fragments arranged by size: 640 484 425 346 333 306 128 114 74 34 9 9 8 PvuII CAG'CTG Cuts at: 0 438 567 714 1017 1089 1263 1807 1896 Size: 438 129 147 303 72 174 544 89 Cuts at: 1896 2910 Size: 1014 Fragments arranged by size: 1014 544 438 303 174 147 129 89 72 SacII CC_GC'GG Cuts at: 0 1142 2910 Size: 1142 1768 SapI GCTCTTCn'nnn_ Cuts at: 0 736 1495 2910 Size: 736 759 1415 Fragments arranged by size: 1415 759 736 SexAI A'CCwGG_T Cuts at: 0 374 1160 2228 2910 Size: 374 786 1068 682 Fragments arranged by size: 1068 786 682 374 SfcI C'TryA_G Cuts at: 0 302 430 439 693 1079 1153 1267 1600 Size: 302 128 9 254 386 74 114 333 Cuts at: 1600 1609 1643 1989 2414 2422 2910 Size: 9 34 346 425 8 488 Fragments arranged by size: 488 425 386 346 333 302 254 128 114 74 34 9 9 8 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2177 2910 Size: 2177 733 SfoI GGC'GCC Cuts at: 0 2740 2910 Size: 2740 170 SmaI CCC'GGG Cuts at: 0 1042 1564 2910 Size: 1042 522 1346 Fragments arranged by size: 1346 1042 522 SmlI C'TyrA_G Cuts at: 0 153 457 2910 Size: 153 304 2453 Fragments arranged by size: 2453 304 153 SphI G_CATG'C Cuts at: 0 1123 1215 2394 2910 Size: 1123 92 1179 516 Fragments arranged by size: 1179 1123 516 92 SrfI GCCC'GGGC Cuts at: 0 1042 2910 Size: 1042 1868 StuI AGG'CCT Cuts at: 0 318 2272 2910 Size: 318 1954 638 Fragments arranged by size: 1954 638 318 StyI C'CwwG_G Cuts at: 0 777 822 1026 1698 1758 2364 2910 Size: 777 45 204 672 60 606 546 Fragments arranged by size: 777 672 606 546 204 60 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 398 2910 Size: 398 2512 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2486 2518 2910 Size: 2486 32 392 Fragments arranged by size: 2486 392 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2010 2910 Size: 2010 900 XmaI C'CCGG_G Cuts at: 0 1040 1562 2910 Size: 1040 522 1348 Fragments arranged by size: 1348 1040 522 XmnI GAAnn'nnTTC Cuts at: 0 2203 2910 Size: 2203 707 Enzymes that do cut: AarI Acc65I AcuI AflII AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacII SapI SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflIII AgeI AhdI AleI AscI AseI AsiSI BaeI BciVI BfrBI BglII BmtI BsaWI BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PvuI RsrII SacI SalI SanDI SbfI ScaI SgfI SgrAI SnaBI SpeI SspI SwaI TatI Tth111I XbaI XhoI ZraI