Restriction map for
pFN21AB3389
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BbrPI BbvCI BcgI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstBI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoRI EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeI MamI MluI MroI
NaeI NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
RcaI RsrII SacI SalI SbfI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1063 GTAGAC GT^[AC][GT]AC
Alw44I 581 GTGCAC G^TGCAC
AocI 1327 CCTGAGG CC^T[ACGT]AGG
ApaI 362 GGGCCC GGGCC^C
ApaLI 581 GTGCAC G^TGCAC
AseI 814 ATTAAT AT^TAAT
AsnI 814 ATTAAT AT^TAAT
AspHI 581 GTGCAC G[AT]GC[AT]^C
AvaI 172 CCCGGG C^[CT]CG[GA]G
BalI 269 TGGCCA TGG^CCA
BbsI 808 GAAGAC GAAGAC(2/6)
BpuAI 808 GAAGAC GAAGAC(2/6)
BsaAI 463 TACGTG [CT]AC^GT[GA]
BseRI 340 GAGGAG GAGGAG(10/8)
BsiHKAI 581 GTGCAC G[AT]GC[AT]^C
BsmAI 247 GTCTC GTCTC(1/5)
BsoBI 172 CCCGGG C^[CT]CG[GA]G
Bsp1407I 972 TGTACA T^GTACA
BsrGI 972 TGTACA T^GTACA
BssSI 214 CTCGTG CACGAG(-5/-1)
BstAPI 378 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 411 GGTGACC G^GT[ACGT]ACC
BstPI 411 GGTGACC G^GT[ACGT]ACC
Bsu36I 1327 CCTGAGG CC^T[ACGT]AGG
DraI 1315 TTTAAA TTT^AAA
EagI 9 CGGCCG C^GGCCG
EclXI 9 CGGCCG C^GGCCG
Eco52I 9 CGGCCG C^GGCCG
Eco81I 1327 CCTGAGG CC^T[ACGT]AGG
EcoO65I 411 GGTGACC G^GT[ACGT]ACC
EcoRV 835 GATATC GAT^ATC
KspI 16 CCGCGG CCGC^GG
MfeI 1292 CAATTG C^AATTG
MluNI 269 TGGCCA TGG^CCA
MscI 269 TGGCCA TGG^CCA
MunI 1292 CAATTG C^AATTG
NdeI 1016 CATATG CA^TATG
NotI 8 GCGGCCGC GC^GGCCGC
NspI 657 GCATGT [GA]CATG^[CT]
PleI 1098 GAGTC GAGTC(4/5)
PpuMI 398 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1066 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 814 ATTAAT AT^TAAT
PstI 418 CTGCAG CTGCA^G
PvuI 531 CGATCG CGAT^CG
PvuII 71 CAGCTG CAG^CTG
SacII 16 CCGCGG CCGC^GG
SapI 423 GCTCTTC GCTCTTC(1/4)
ScaI 1310 AGTACT AGT^ACT
SfaNI 345 GCATC GCATC(5/9)
SmaI 172 CCCGGG CCC^GGG
SspBI 972 TGTACA T^GTACA
SspI 992 AATATT AAT^ATT
XmaCI 172 CCCGGG C^CCGGG
XmaI 172 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 17 CGCG CG^CG
161 CGCG
AcyI 167 GGCGCC G[GA]^CG[CT]C
201 GGCGCC
AlwNI 71 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1102 CAGTGGCTG
AvaII 20 GGTCC G^G[AT]CC
399 GGACC
BbeI 167 GGCGCC GGCGC^C
201 GGCGCC
BciVI 1000 GGATAC GTATCC(6/5)
1077 GTATCC
BpmI 108 CTCCAG CTGGAG(16/14)
856 CTCCAG
BsaHI 167 GGCGCC G[GA]^CG[CT]C
201 GGCGCC
BsiEI 9 CGGCCG CG[GA][CT]^CG
531 CGATCG
BspMI 383 ACCTGC ACCTGC(4/8)
1048 GCAGGT
BsrBI 125 CCGCTC CCGCTC(-3/-3)
52 GAGCGG
BstUI 17 CGCG CG^CG
161 CGCG
EaeI 9 CGGCCG [CT]^GGCC[GA]
269 TGGCCA
Hin1I 167 GGCGCC G[GA]^CG[CT]C
201 GGCGCC
KasI 167 GGCGCC G^GCGCC
201 GGCGCC
MslI 574 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1033 CATTTCAATG
MspA1I 16 CCGCGG C[AC]G^C[GT]G
71 CAGCTG
MvnI 17 CGCG CG^CG
161 CGCG
NarI 167 GGCGCC GG^CGCC
201 GGCGCC
NcoI 266 CCATGG C^CATGG
476 CCATGG
SfoI 167 GGCGCC GGC^GCC
201 GGCGCC
Tsp45I 322 GTCAC ^GT[GC]AC
412 GTGAC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BamHI BanI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII