(Circular) (Six-base) MAPSORT of: pFN21AB3389.seq Check: 4976 from: 1 to: 6061 pFN21AB3389 6061 bp With 182 enzymes: SgfI * June 11, 2014 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2397 3044 2397 Size: 647 5414 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3069 3404 1937 Size: 1132 335 4594 Fragments arranged by size: 4594 1132 335 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3180 4124 5508 5776 1632 Size: 1548 944 1384 268 1917 Fragments arranged by size: 1917 1548 1384 944 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2081 3112 3804 5376 2081 Size: 1031 692 1572 2766 Fragments arranged by size: 2766 1572 1031 692 ApaI G_GGCC'C Cuts at: 2371 2371 Size: 6061 ApaLI G'TGCA_C Cuts at: 2586 4118 5274 2586 Size: 1532 1156 3373 Fragments arranged by size: 3373 1532 1156 ApoI r'AATT_y Cuts at: 1493 2044 2505 2630 3047 3140 3370 3596 3748 Size: 551 461 125 417 93 230 226 152 Cuts at: 3748 5857 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 551 461 417 230 226 152 125 93 AseI AT'TA_AT Cuts at: 160 1032 2820 4615 160 Size: 872 1788 1795 1606 Fragments arranged by size: 1795 1788 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2177 3386 1870 Size: 73 234 1209 4545 Fragments arranged by size: 4545 1209 234 73 BamHI G'GATC_C Cuts at: 2189 2653 2742 3391 3758 2189 Size: 464 89 649 367 4492 Fragments arranged by size: 4492 649 464 367 89 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2172 2206 2311 3382 4837 Size: 325 217 627 385 34 105 1071 1455 Cuts at: 4837 618 Size: 1842 Fragments arranged by size: 1842 1455 1071 627 385 325 217 105 34 BanII G_rGCy'C Cuts at: 1391 2070 2371 2909 3380 1391 Size: 679 301 538 471 4072 Fragments arranged by size: 4072 679 538 471 301 BbeI G_GCGC'C Cuts at: 2176 2210 2176 Size: 34 6027 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2820 928 Size: 971 921 4169 Fragments arranged by size: 4169 971 921 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4252 4286 1903 Size: 34 50 34 2231 34 3678 Fragments arranged by size: 3678 2231 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2999 3093 3953 5163 2999 Size: 94 860 1210 3897 Fragments arranged by size: 3897 1210 860 94 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2316 2371 2590 4122 5278 1790 Size: 526 55 219 1532 1156 2573 Fragments arranged by size: 2573 1532 1156 526 219 55 BmgBI CAC'GTC Cuts at: 1318 2290 1318 Size: 972 5089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2410 3792 4750 Size: 416 53 83 399 24 993 1382 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1753 1382 993 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2846 4721 1239 Size: 859 748 1875 2579 Fragments arranged by size: 2579 1875 859 748 Bpu10I CC'TnA_GC Cuts at: 2372 2372 Size: 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2470 3852 493 Size: 711 1266 1382 2702 Fragments arranged by size: 2702 1382 1266 711 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2173 2207 3868 4250 Size: 53 83 186 936 640 34 1661 382 Cuts at: 4250 4885 275 Size: 635 1451 Fragments arranged by size: 1661 1451 936 640 635 382 186 83 53 34 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2360 1186 Size: 1174 4887 BseYI C'CCAG_C Cuts at: 2146 2155 2674 5264 5760 2146 Size: 9 519 2590 496 2447 Fragments arranged by size: 2590 2447 519 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2017 2539 3511 4272 5300 1540 Size: 275 186 16 522 972 761 1028 2301 Fragments arranged by size: 2301 1028 972 761 522 275 186 16 BsiHKAI G_wGCw'C Cuts at: 2590 4122 4207 5278 2590 Size: 1532 85 1071 3373 Fragments arranged by size: 3373 1532 1071 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2316 2371 2590 2909 3380 4122 Size: 399 280 246 55 219 319 471 742 Cuts at: 4122 4207 5278 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 319 280 246 219 85 55 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2397 3044 3416 844 Size: 1553 647 372 3489 Fragments arranged by size: 3489 1553 647 372 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2132 3948 1394 Size: 429 236 73 1816 3507 Fragments arranged by size: 3507 1816 429 236 73 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2977 96 Size: 2881 3180 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 2219 4121 5133 2219 Size: 1902 1012 3147 Fragments arranged by size: 3147 1902 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2389 794 Size: 1595 4466 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 2416 1184 Size: 1232 4829 BstYI r'GATC_y Cuts at: 1972 2189 2653 2742 3391 3758 4143 4160 5612 Size: 217 464 89 649 367 385 17 1452 Cuts at: 5612 5698 5710 6056 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 649 464 385 367 346 217 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3333 3357 3333 Size: 24 6037 BtgI C'CryG_G Cuts at: 513 1065 2021 2271 2481 513 Size: 552 956 250 210 4093 Fragments arranged by size: 4093 956 552 250 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2336 487 Size: 683 56 1110 4212 Fragments arranged by size: 4212 1110 683 56 BtsI GCAGTG_nn' Cuts at: 3577 4371 4391 3577 Size: 794 20 5247 Fragments arranged by size: 5247 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2785 2820 529 Size: 35 2221 35 3770 Fragments arranged by size: 3770 2221 35 35 DraI TTT'AAA Cuts at: 3322 3365 3322 Size: 43 6018 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2014 2274 3508 Size: 54 1425 50 275 18 184 260 1234 Cuts at: 3508 4397 6026 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1234 889 275 260 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2014 3508 1537 Size: 275 202 1494 4090 Fragments arranged by size: 4090 1494 275 202 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 2862 3194 3991 1353 Size: 1082 427 332 797 3423 Fragments arranged by size: 3423 1082 797 427 332 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2245 4648 5022 5168 387 Size: 1064 138 656 2403 374 146 1280 Fragments arranged by size: 2403 1280 1064 656 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2846 3180 4124 4721 5508 5776 Size: 393 466 748 334 944 597 787 268 Cuts at: 5776 1239 Size: 1524 Fragments arranged by size: 1524 944 787 748 597 466 393 334 268 EcoO109I rG'GnC_Cy Cuts at: 2367 2368 2404 2367 Size: 1 36 6024 Fragments arranged by size: 6024 36 1 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 EcoRV GAT'ATC Cuts at: 2842 2842 Size: 6061 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 2153 2176 2210 5208 1678 Size: 475 23 34 2998 2531 Fragments arranged by size: 2998 2531 475 34 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373 Size: 32 135 32 3130 32 42 32 2626 Fragments arranged by size: 3130 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KasI G'GCGC_C Cuts at: 2172 2206 2172 Size: 34 6027 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3297 3660 3297 Size: 363 5698 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 2922 5175 5359 1305 Size: 753 864 2253 184 2007 Fragments arranged by size: 2253 2007 864 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 10 Size: 54 1425 343 444 3795 Fragments arranged by size: 3795 1425 444 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2583 3042 4019 4378 4537 518 Size: 648 1417 459 977 359 159 2042 Fragments arranged by size: 2042 1417 977 648 459 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2078 3477 4154 5302 5547 1606 Size: 417 55 1399 677 1148 245 2120 Fragments arranged by size: 2120 1399 1148 677 417 245 55 NarI GG'CG_CC Cuts at: 2173 2207 2173 Size: 34 6027 NcoI C'CATG_G Cuts at: 513 1065 2271 2481 513 Size: 552 1206 210 4093 Fragments arranged by size: 4093 1206 552 210 NdeI CA'TA_TG Cuts at: 387 3022 387 Size: 2635 3426 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 2014 3508 2014 Size: 1494 4567 NspI r_CATG'y Cuts at: 1132 1229 2666 3419 4964 1132 Size: 97 1437 753 1545 2229 Fragments arranged by size: 2229 1545 1437 753 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PfoI T'CCnGG_A Cuts at: 2862 2862 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2404 2404 Size: 6061 PshAI GACnn'nnGTC Cuts at: 1613 3075 1613 Size: 1462 4599 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2367 2367 Size: 6061 PstI C_TGCA'G Cuts at: 830 2427 3413 830 Size: 1597 986 3478 Fragments arranged by size: 3478 1597 986 PvuI CG_AT'CG Cuts at: 2001 2539 2001 Size: 538 5523 PvuII CAG'CTG Cuts at: 2078 2078 Size: 6061 SacII CC_GC'GG Cuts at: 2024 2024 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SanDI GG'GwC_CC Cuts at: 2404 2404 Size: 6061 SapI GCTCTTCn'nnn_ Cuts at: 2435 2435 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 3317 4309 6021 3317 Size: 992 1712 3357 Fragments arranged by size: 3357 1712 992 SfcI C'TryA_G Cuts at: 826 1045 2423 3073 3098 3409 4544 5225 5416 Size: 219 1378 650 25 311 1135 681 191 Cuts at: 5416 826 Size: 1471 Fragments arranged by size: 1471 1378 1135 681 650 311 219 191 25 SfoI GGC'GCC Cuts at: 2174 2208 2174 Size: 34 6027 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 2179 3388 1872 Size: 307 1209 4545 Fragments arranged by size: 4545 1209 307 SmlI C'TyrA_G Cuts at: 820 1017 1943 4165 5066 5328 820 Size: 197 926 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 2999 3985 5 Size: 47 2947 986 2081 Fragments arranged by size: 2947 2081 986 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2271 2481 3286 3500 513 Size: 552 1206 210 805 214 3074 Fragments arranged by size: 3074 1206 805 552 214 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2571 4088 4105 4258 4443 4888 325 Size: 1255 991 1517 17 153 185 445 1498 Fragments arranged by size: 1517 1498 1255 991 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2977 3315 4307 Size: 274 80 33 51 1443 1000 338 992 Cuts at: 4307 6019 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1000 992 338 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 2177 3386 1870 Size: 307 1209 4545 Fragments arranged by size: 4545 1209 307 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI XcmI