(Circular) (Six-base) MAPSORT of: pFN21AE2389.seq Check: 9462 from: 1 to: 6778 pFN21AE2389 6778 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4588 278 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3876 4121 1937 Size: 1939 245 4594 Fragments arranged by size: 4594 1939 245 Acc65I G'GTAC_C Cuts at: 4099 4099 Size: 6778 AclI AA'CG_TT Cuts at: 1563 4905 5278 1563 Size: 3342 373 3063 Fragments arranged by size: 3342 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 4841 6225 6493 1632 Size: 402 2807 1384 268 1917 Fragments arranged by size: 2807 1917 1384 402 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6581 AflIII A'CryG_T Cuts at: 1203 2511 3667 3797 3830 5677 6654 1203 Size: 1308 1156 130 33 1847 977 1327 Fragments arranged by size: 1847 1327 1308 1156 977 130 33 AgeI A'CCGG_T Cuts at: 5609 5609 Size: 6778 AhdI GACnn_n'nnGTC Cuts at: 5507 5507 Size: 6778 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6778 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6778 AlwNI CAG_nnn'CTG Cuts at: 4521 6093 4521 Size: 1572 5206 ApaLI G'TGCA_C Cuts at: 4835 5991 4835 Size: 1156 5622 ApoI r'AATT_y Cuts at: 1493 2233 2404 2442 3200 3230 3602 3634 3756 Size: 740 171 38 758 30 372 32 122 Cuts at: 3756 4087 4313 4465 6574 1493 Size: 331 226 152 2109 1697 Fragments arranged by size: 2109 1697 758 740 372 331 226 171 152 122 38 32 30 AseI AT'TA_AT Cuts at: 160 1032 2436 3932 5332 160 Size: 872 1404 1496 1400 1606 Fragments arranged by size: 1606 1496 1404 1400 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 AvaI C'yCGr_G Cuts at: 1870 1943 4103 1870 Size: 73 2160 4545 Fragments arranged by size: 4545 2160 73 BamHI G'GATC_C Cuts at: 2267 4108 4475 2267 Size: 1841 367 4570 Fragments arranged by size: 4570 1841 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3659 4099 5554 618 Size: 325 217 627 1872 440 1455 1842 Fragments arranged by size: 1872 1842 1455 627 440 325 217 BanII G_rGCy'C Cuts at: 1391 3784 4097 1391 Size: 2393 313 4072 Fragments arranged by size: 4072 2393 313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3073 3882 928 Size: 971 1174 809 3824 Fragments arranged by size: 3824 1174 971 809 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4969 5003 1903 Size: 34 3032 34 3678 Fragments arranged by size: 3678 3032 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4670 5880 4670 Size: 1210 5568 BclI T'GATC_A Cuts at: 1548 1548 Size: 6778 BfrBI ATG'CAT Cuts at: 3977 3977 Size: 6778 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5389 136 Size: 107 122 71 631 605 3717 1525 Fragments arranged by size: 3717 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2725 6773 2725 Size: 4048 2730 BlpI GC'TnA_GC Cuts at: 4195 4195 Size: 6778 Bme1580I G_kGCm'C Cuts at: 1790 4839 5995 1790 Size: 3049 1156 2573 Fragments arranged by size: 3049 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6778 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3728 4509 5467 Size: 416 53 83 399 24 2311 781 958 Cuts at: 5467 442 Size: 1753 Fragments arranged by size: 2311 1753 958 781 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6778 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 6778 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3524 5438 1239 Size: 2285 1914 2579 Fragments arranged by size: 2579 2285 1914 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4903 5768 6066 4903 Size: 865 298 5615 Fragments arranged by size: 5615 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3542 5441 882 Size: 617 2043 1899 2219 Fragments arranged by size: 2219 2043 1899 617 BsaAI yAC'GTr Cuts at: 493 1204 2707 3091 3833 4569 493 Size: 711 1503 384 742 736 2702 Fragments arranged by size: 2702 1503 742 736 711 384 BsaBI GATnn'nnATC Cuts at: 6772 6772 Size: 6778 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4585 4967 5602 275 Size: 53 83 186 936 3052 382 635 1451 Fragments arranged by size: 3052 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5211 5609 5883 6030 6441 5211 Size: 398 274 147 411 5548 Fragments arranged by size: 5548 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6553 Fragments arranged by size: 6553 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6778 BseYI C'CCAG_C Cuts at: 4052 5981 6477 4052 Size: 1929 496 4353 Fragments arranged by size: 4353 1929 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3633 1163 Size: 573 1897 4308 Fragments arranged by size: 4308 1897 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4228 4989 6017 1540 Size: 275 186 2227 761 1028 2301 Fragments arranged by size: 2301 2227 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3748 4839 4924 5995 3748 Size: 1091 85 1071 4531 Fragments arranged by size: 4531 1091 1071 85 BsmI GAATG_Cn' Cuts at: 2647 3578 4289 4382 2647 Size: 931 711 93 5043 Fragments arranged by size: 5043 931 711 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3748 3784 4097 4839 4924 5995 1391 Size: 399 1958 36 313 742 85 1071 2174 Fragments arranged by size: 2174 1958 1071 742 399 313 85 36 BspEI T'CCGG_A Cuts at: 6441 6441 Size: 6778 BspHI T'CATG_A Cuts at: 4667 6397 4667 Size: 1730 5048 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3455 4133 844 Size: 2611 678 3489 Fragments arranged by size: 3489 2611 678 BsrBI CCG'CTC Cuts at: 1394 1823 4665 1394 Size: 429 2842 3507 Fragments arranged by size: 3507 2842 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2083 2658 5273 5447 65 Size: 1183 835 575 2615 174 1396 Fragments arranged by size: 2615 1396 1183 835 575 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5422 5609 1700 Size: 110 3612 187 2869 Fragments arranged by size: 3612 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3593 96 Size: 3497 3281 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6778 BssSI C'ACGA_G Cuts at: 4838 5850 4838 Size: 1012 5766 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6778 BstBI TT'CG_AA Cuts at: 5582 5582 Size: 6778 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6778 BstYI r'GATC_y Cuts at: 1972 2267 2684 2725 4108 4475 4860 4877 6329 Size: 295 417 41 1383 367 385 17 1452 Cuts at: 6329 6415 6427 6773 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1383 417 385 367 346 295 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5287 Fragments arranged by size: 5287 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6039 Fragments arranged by size: 6039 683 56 BtsI GCAGTG_nn' Cuts at: 4294 5088 5108 4294 Size: 794 20 5964 Fragments arranged by size: 5964 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3805 3840 529 Size: 35 3241 35 3467 Fragments arranged by size: 3467 3241 35 35 DraI TTT'AAA Cuts at: 2493 2992 4082 2493 Size: 499 1090 5189 Fragments arranged by size: 5189 1090 499 DraIII CAC_nnn'GTG Cuts at: 2135 4076 2135 Size: 1941 4837 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5785 809 Size: 507 4469 1802 Fragments arranged by size: 4469 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4225 5114 6743 Size: 54 1425 50 275 18 2395 889 1629 Cuts at: 6743 8 Size: 43 Fragments arranged by size: 2395 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4225 1537 Size: 275 2413 4090 Fragments arranged by size: 4090 2413 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2197 4708 1353 Size: 844 2511 3423 Fragments arranged by size: 3423 2511 844 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5365 5739 5885 387 Size: 1064 138 3776 374 146 1280 Fragments arranged by size: 3776 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 3524 4841 5438 6225 6493 1239 Size: 393 402 1490 1317 597 787 268 1524 Fragments arranged by size: 1524 1490 1317 787 597 402 393 268 EcoRI G'AATT_C Cuts at: 3756 4087 4465 3756 Size: 331 378 6069 Fragments arranged by size: 6069 378 331 EcoRV GAT'ATC Cuts at: 2187 3970 2187 Size: 1783 4995 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2110 2895 2110 Size: 785 5993 FspI TGC'GCA Cuts at: 5284 5284 Size: 6778 HaeII r_GCGC'y Cuts at: 1678 5925 1678 Size: 4247 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3174 3206 5419 5451 5493 Size: 32 135 32 1602 32 2213 32 42 Cuts at: 5493 5525 1373 Size: 32 2626 Fragments arranged by size: 2626 2213 1602 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4122 4368 669 Size: 988 281 2184 246 3079 Fragments arranged by size: 3079 2184 988 281 246 HindIII A'AGCT_T Cuts at: 748 2218 2877 748 Size: 1470 659 4649 Fragments arranged by size: 4649 1470 659 HpaI GTT'AAC Cuts at: 4368 4368 Size: 6778 KpnI G_GTAC'C Cuts at: 4103 4103 Size: 6778 MfeI C'AATT_G Cuts at: 3394 4377 3394 Size: 983 5795 MluI A'CGCG_T Cuts at: 6654 6654 Size: 6778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2749 3199 5892 6076 1305 Size: 1444 450 2693 184 2007 Fragments arranged by size: 2693 2007 1444 450 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4956 Fragments arranged by size: 4956 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2512 3360 3567 4736 5095 5254 518 Size: 648 1346 848 207 1169 359 159 2042 Fragments arranged by size: 2042 1346 1169 848 648 359 207 159 MspA1I CmG'CkG Cuts at: 1606 4194 4871 6019 6264 1606 Size: 2588 677 1148 245 2120 Fragments arranged by size: 2588 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5287 Fragments arranged by size: 5287 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6778 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6778 NotI GC'GGCC_GC Cuts at: 4225 4225 Size: 6778 NsiI A_TGCA'T Cuts at: 3979 3979 Size: 6778 NspI r_CATG'y Cuts at: 1132 1229 2515 2897 3671 4136 5681 1132 Size: 97 1286 382 774 465 1545 2229 Fragments arranged by size: 2229 1545 1286 774 465 382 97 PacI TTA_AT'TAA Cuts at: 2436 2436 Size: 6778 PasI CC'CwG_GG Cuts at: 1348 2680 1348 Size: 1332 5446 PciI A'CATG_T Cuts at: 2511 3667 5677 2511 Size: 1156 2010 3612 Fragments arranged by size: 3612 2010 1156 PflMI CCAn_nnn'nTGG Cuts at: 1108 2669 3425 3733 1108 Size: 1561 756 308 4153 Fragments arranged by size: 4153 1561 756 308 PmeI GTTT'AAAC Cuts at: 4082 4082 Size: 6778 PmlI CAC'GTG Cuts at: 3833 3833 Size: 6778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4847 4879 6360 6392 4847 Size: 32 1481 32 5233 Fragments arranged by size: 5233 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6778 PsiI TTA'TAA Cuts at: 4348 4348 Size: 6778 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3536 3568 3536 Size: 32 6746 PstI C_TGCA'G Cuts at: 830 3518 4130 830 Size: 2688 612 3478 Fragments arranged by size: 3478 2688 612 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6778 SalI G'TCGA_C Cuts at: 1936 4120 1936 Size: 2184 4594 SbfI CC_TGCA'GG Cuts at: 4130 4130 Size: 6778 ScaI AGT'ACT Cuts at: 5026 6738 5026 Size: 1712 5066 SfcI C'TryA_G Cuts at: 826 1045 3498 3514 4126 5261 5942 6133 826 Size: 219 2453 16 612 1135 681 191 1471 Fragments arranged by size: 2453 1471 1135 681 612 219 191 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6778 SmaI CCC'GGG Cuts at: 1872 4105 1872 Size: 2233 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4882 5783 6045 820 Size: 197 926 2939 901 262 1553 Fragments arranged by size: 2939 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3091 4569 493 Size: 2598 1478 2702 Fragments arranged by size: 2702 2598 1478 SpeI A'CTAG_T Cuts at: 152 152 Size: 6778 SphI G_CATG'C Cuts at: 1132 4136 1132 Size: 3004 3774 SspI AAT'ATT Cuts at: 5 52 2956 4702 5 Size: 47 2904 1746 2081 Fragments arranged by size: 2904 2081 1746 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6778 StyI C'CwwG_G Cuts at: 513 1065 2004 4217 513 Size: 552 939 2213 3074 Fragments arranged by size: 3074 2213 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3557 4805 4822 4975 5160 5605 325 Size: 1255 1977 1248 17 153 185 445 1498 Fragments arranged by size: 1977 1498 1255 1248 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3593 5024 6736 Size: 274 80 33 51 1443 1616 1431 1712 Cuts at: 6736 96 Size: 138 Fragments arranged by size: 1712 1616 1443 1431 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3708 3740 6738 6770 3708 Size: 32 2998 32 3716 Fragments arranged by size: 3716 2998 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6778 XbaI T'CTAG_A Cuts at: 4114 4114 Size: 6778 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 2572 Size: 6778 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6778 XmaI C'CCGG_G Cuts at: 1870 4103 1870 Size: 2233 4545 XmnI GAAnn'nnTTC Cuts at: 2090 2878 4907 2090 Size: 788 2029 3961 Fragments arranged by size: 3961 2029 788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4586 276 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI