################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## Acc65I AccI AccII AccIII AciI AclI AfaI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HgaI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MfeI MluI MroI MseI MslI MspA1I MunI MvaI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 545 GACGTC GACGT^C AcsI 199 AAATTC [GA]^AATT[CT] AcyI 545 GACGTC G[GA]^CG[CT]C AlwI 556 GGATC GGATC(4/5) AlwNI 469 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 288 AGCGCT AGC^GCT ApoI 199 AAATTC [GA]^AATT[CT] BalI 569 TGGCCA TGG^CCA BbsI 267 GTCTTC GAAGAC(2/6) BbvI 460 GCAGC GCAGC(8/12) BclI 578 TGATCA T^GATCA BcnI 306 CCGGG CC^[GC]GG BglII 375 AGATCT A^GATCT BpmI 475 CTGGAG CTGGAG(16/14) BpuAI 267 GTCTTC GAAGAC(2/6) BsaHI 545 GACGTC G[GA]^CG[CT]C BsmBI 543 GAGACG CGTCTC(1/5) BsmFI 548 GTCCC GGGAC(10/14) BssKI 306 CCGGG ^CC[ACGT]GG BstF5I 513 CATCC GGATG(2/0) Cac8I 420 GCTGGC GC[ACGT]^[ACGT]GC CfoI 289 GCGC GCG^C DdeI 77 CTAAG C^T[ACGT]AG EaeI 569 TGGCCA [CT]^GGCC[GA] Eco47III 288 AGCGCT AGC^GCT FbaI 578 TGATCA T^GATCA Fnu4HI 460 GCAGC GC^[ACGT]GC FokI 513 CATCC GGATG(9/13) HaeII 288 AGCGCT [GA]GCGC^[CT] HaeIII 570 GGCC GG^CC HapII 306 CCGG C^CGG HhaI 289 GCGC GCG^C Hin1I 545 GACGTC G[GA]^CG[CT]C HinP1I 289 GCGC G^CGC HpaII 306 CCGG C^CGG ItaI 460 GCAGC GC^[ACGT]GC MaeII 546 ACGT A^CGT MaeIII 46 GTTAC ^GT[ACGT]AC MluNI 569 TGGCCA TGG^CCA MscI 569 TGGCCA TGG^CCA MspI 306 CCGG C^CGG NciI 306 CCGGG CC^[GC]GG NlaIII 385 CATG CATG^ NlaIV 309 GGAGCC GG[ACGT]^[ACGT]CC PstI 458 CTGCAG CTGCA^G ScrFI 306 CCGGG CC^[ACGT]GG SpeI 319 ACTAGT A^CTAGT TseI 460 GCAGC G^C[AT]GC XbaI 159 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BfaI 160 CTAG C^TAG 320 CTAG BsaBI 489 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 557 GATCTAAATC BsmAI 543 GAGAC GTCTC(1/5) 471 GTCTC BstYI 375 AGATCT [GA]^GATC[CT] 556 GGATCT MaeI 160 CTAG C^TAG 320 CTAG MamI 489 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 557 GATCTAAATC MboII 406 GAAGA GAAGA(8/7) 268 TCTTC MflI 375 AGATCT [GA]^GATC[CT] 556 GGATCT SfaNI 437 GATGC GCATC(5/9) 15 GCATC SfcI 297 CTACAG C^T[GA][CT]AG 458 CTGCAG XhoII 375 AGATCT [GA]^GATC[CT] 556 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BsrI DpnII HinfI MboI MnlI NdeII Sau3AI TfiI Tsp509I TspRI