################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 247 GTCTAC GT^[AC][GT]AC AciI 13 GCGG CCGC(-3/-1) AflII 292 CTTAAG C^TTAAG AlwI 106 GATCC GGATC(4/5) AspHI 438 GTGCTC G[AT]GC[AT]^C AvaII 366 GGACC G^G[AT]CC BalI 407 TGGCCA TGG^CCA BcgI 435 CGAGTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 292 CTTAAG C^TTAAG BsiHKAI 438 GTGCTC G[AT]GC[AT]^C BspDI 145 ATCGAT AT^CGAT BspMI 378 GCAGGT ACCTGC(4/8) BssKI 374 CCAGG ^CC[ACGT]GG BstNI 374 CCAGG CC^[AT]GG BstXI 410 CCACAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 366 GGACC G^G[ACGT]CC ClaI 145 ATCGAT AT^CGAT DsaI 164 CCATGG C^C[GA][CT]GG EaeI 407 TGGCCA [CT]^GGCC[GA] EcoRII 374 CCAGG ^CC[AT]GG EcoT14I 164 CCATGG C^C[AT][AT]GG HaeIII 408 GGCC GG^CC HapII 507 CCGG C^CGG HpaII 507 CCGG C^CGG HphI 581 TCACC GGTGA(8/7) MaeIII 331 GTGAC ^GT[ACGT]AC MluNI 407 TGGCCA TGG^CCA MscI 407 TGGCCA TGG^CCA MspI 507 CCGG C^CGG MvaI 374 CCAGG CC^[AT]GG NcoI 164 CCATGG C^CATGG PleI 333 GACTC GAGTC(4/5) Sau96I 366 GGACC G^G[ACGT]CC ScrFI 374 CCAGG CC^[ACGT]GG SfaNI 576 GATGC GCATC(5/9) SmlI 292 CTTAAG C^T[CT][GA]AG SspI 30 AATATT AAT^ATT StyI 164 CCATGG C^C[AT][AT]GG TfiI 118 GAATC G^A[AT]TC Tsp45I 331 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### BbvI 539 GCAGC GCAGC(8/12) 285 GCTGC BfaI 175 CTAG C^TAG 304 CTAG BmyI 214 GGGCAC G[AGT]GC[ACT]^C 438 GTGCTC BpmI 372 CTCCAG CTGGAG(16/14) 452 CTGGAG BsaJI 164 CCATGG C^C[ACGT][ACGT]GG 264 CCGAGG BsgI 447 CTGCAC GTGCAG(16/14) 517 GTGCAG BsiYI 200 CCATTCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 507 CCGGAGGATGG BslI 200 CCATTCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 507 CCGGAGGATGG Bsp1286I 214 GGGCAC G[AGT]GC[ACT]^C 438 GTGCTC BsrI 24 ACTGG ACTGG(1/-1) 451 ACTGG BstF5I 300 CATCC GGATG(2/0) 512 GGATG DpnII 106 GATC ^GATC 312 GATC Fnu4HI 285 GCTGC GC^[ACGT]GC 539 GCAGC FokI 300 CATCC GGATG(9/13) 512 GGATG HgaI 536 GACGC GACGC(5/10) 501 GCGTC HinfI 118 GAATC G^A[ACGT]TC 333 GACTC ItaI 285 GCTGC GC^[ACGT]GC 539 GCAGC MaeI 175 CTAG C^TAG 304 CTAG MboI 106 GATC ^GATC 312 GATC MboII 193 GAAGA GAAGA(8/7) 391 GAAGA MseI 293 TTAA T^TAA 569 TTAA NdeII 106 GATC ^GATC 312 GATC NspI 218 ACATGC [GA]CATG^[CT] 552 GCATGT Sau3AI 106 GATC ^GATC 312 GATC Tru9I 293 TTAA T^TAA 569 TTAA TseI 285 GCTGC G^C[AT]GC 539 GCAGC Tsp509I 416 AATT ^AATT 485 AATT TspRI 180 CACTG CA[GC]TG[ACGT][ACGT]^ 450 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AluI Cac8I MnlI MwoI NlaIII TaqI TthHB8I