##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FauI        FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MboI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
TseI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AspHI         226  GAGCTC               G[AT]GC[AT]^C
AvaII          43  GGTCC                G^G[AT]CC
BanII         226  GAGCTC               G[GA]GC[CT]^C
BfaI          166  CTAG                 C^TAG
BmyI          226  GAGCTC               G[AGT]GC[ACT]^C
BpmI           26  CTCCAG               CTGGAG(16/14)
BsiHKAI       226  GAGCTC               G[AT]GC[AT]^C
BsmI          202  GCATTC               GAATGC(1/-1)
Bsp1286I      226  GAGCTC               G[AGT]GC[ACT]^C
DdeI          233  CTCAG                C^T[ACGT]AG
DsaI          193  CCATGG               C^C[GA][CT]GG
EcoRV         175  GATATC               GAT^ATC
EcoT14I       193  CCATGG               C^C[AT][AT]GG
HaeIII         64  GGCC                 GG^CC
HinfI          90  GACTC                G^A[ACGT]TC
MaeI          166  CTAG                 C^TAG
MaeIII          5  GTGAC                ^GT[ACGT]AC
NcoI          193  CCATGG               C^CATGG
NlaIV          42  GGGTCC               GG[ACGT]^[ACGT]CC
PleI           90  GACTC                GAGTC(4/5)
PpuMI          42  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI          226  GAGCTC               GAGCT^C
StyI          193  CCATGG               C^C[AT][AT]GG
Tsp45I          5  GTGAC                ^GT[GC]AC
TspRI         182  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI           30  AGCT                 AG^CT
              227  AGCT                
BsaJI          39  CCAGGG               C^C[ACGT][ACGT]GG
              193  CCATGG              
BsiYI          55  CCACGCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              115  CCAAAGAAAGG         
BslI           55  CCACGCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              115  CCAAAGAAAGG         
Cac8I          21  GCCTGC               GC[ACGT]^[ACGT]GC
              228  GCTCGC              
Cfr13I         43  GGTCC                G^G[ACGT]CC
               64  GGCCC               
DraII          42  GGGTCCT              [GA]G^G[ACGT]CC[CT]
               63  AGGCCCT             
EcoO109I       42  GGGTCCT              [GA]G^G[ACGT]CC[CT]
               63  AGGCCCT             
NlaIII        194  CATG                 CATG^
              223  CATG                
Sau96I         43  GGTCC                G^G[ACGT]CC
               64  GGCCC               
Tsp509I        98  AATT                 ^AATT
              218  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

BssKI       BstNI       EcoRII      MnlI        MvaI        
ScrFI