################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 806 GTAGAC GT^[AC][GT]AC AcyI 49 GACGCC G[GA]^CG[CT]C AspI 715 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 896 CCCGAG C^[CT]CG[GA]G BalI 86 TGGCCA TGG^CCA BbvCI 913 GCTGAGG CCTCAGC(-5/-2) BcnI 717 CCCGG CC^[GC]GG BfaI 340 CTAG C^TAG BsaHI 49 GACGCC G[GA]^CG[CT]C BsiEI 719 CGGTCG CG[GA][CT]^CG BsiWI 270 CGTACG C^GTACG BsmBI 558 CGTCTC CGTCTC(1/5) BsmI 788 GCATTC GAATGC(1/-1) BsoBI 896 CCCGAG C^[CT]CG[GA]G BspHI 452 TCATGA T^CATGA BstAPI 830 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 738 GGTCACC G^GT[ACGT]ACC BstPI 738 GGTCACC G^GT[ACGT]ACC DsaI 61 CCATGG C^C[GA][CT]GG EaeI 86 TGGCCA [CT]^GGCC[GA] EcoNI 820 CCTTTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 738 GGTCACC G^GT[ACGT]ACC EcoT14I 61 CCATGG C^C[AT][AT]GG FauI 684 CCCGC CCCGC(4/6) HapII 718 CCGG C^CGG HgaI 49 GACGC GACGC(5/10) Hin1I 49 GACGCC G[GA]^CG[CT]C HpaII 718 CCGG C^CGG HphI 740 TCACC GGTGA(8/7) MaeI 340 CTAG C^TAG MluNI 86 TGGCCA TGG^CCA MscI 86 TGGCCA TGG^CCA MseI 931 TTAA T^TAA MspI 718 CCGG C^CGG NciI 717 CCCGG CC^[GC]GG NcoI 61 CCATGG C^CATGG NdeI 938 CATATG CA^TATG NlaIV 57 GGGGCC GG[ACGT]^[ACGT]CC PflFI 715 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 743 CCAACGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 159 CTGCAG CTGCA^G RcaI 452 TCATGA T^CATGA StyI 61 CCATGG C^C[AT][AT]GG Tru9I 931 TTAA T^TAA Tth111I 715 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 743 CCAACGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 685 CCGC CCGC(-3/-1) 958 CCGC Bsp1407I 2 TGTACA T^GTACA 767 TGTACA BsrGI 2 TGTACA T^GTACA 767 TGTACA BstF5I 990 CATCC GGATG(2/0) 921 GGATG BstYI 149 AGATCC [GA]^GATC[CT] 659 GGATCT Cac8I 491 GCCAGC GC[ACGT]^[ACGT]GC 835 GCCTGC DrdI 712 GACGACCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 715 GACCCGGTCGTC FokI 990 CATCC GGATG(9/13) 921 GGATG MflI 149 AGATCC [GA]^GATC[CT] 659 GGATCT MslI 645 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 977 CACAAGAGTG SapI 216 GCTCTTC GCTCTTC(1/4) 630 GCTCTTC SfcI 159 CTGCAG C^T[GA][CT]AG 795 CTACAG SspBI 2 TGTACA T^GTACA 767 TGTACA TaqI 17 TCGA T^CGA 245 TCGA Tsp509I 111 AATT ^AATT 384 AATT TthHB8I 17 TCGA T^CGA 245 TCGA XhoII 149 AGATCC [GA]^GATC[CT] 659 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AfaI AluI AlwI AspHI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI HaeIII HinfI ItaI Ksp632I MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI