(Circular) (Six-base) MAPSORT of: pFN21AE9440.seq Check: 5662 from: 1 to: 5740 pFN21AE9440 5740 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2812 3083 1937 Size: 875 271 4594 Fragments arranged by size: 4594 875 271 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3803 5187 5455 1632 Size: 2171 1384 268 1917 Fragments arranged by size: 2171 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 2986 1166 Size: 1820 3920 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 1184 Size: 1251 4489 AlwNI CAG_nnn'CTG Cuts at: 3483 5055 3483 Size: 1572 4168 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 3049 3275 3427 5536 1493 Size: 1556 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2901 3065 1870 Size: 73 958 164 4545 Fragments arranged by size: 4545 958 164 73 BamHI G'GATC_C Cuts at: 3070 3437 3070 Size: 367 5373 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 2223 2256 2790 2892 2902 3059 1391 Size: 832 33 534 102 10 157 4072 Fragments arranged by size: 4072 832 534 157 102 33 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BbvCI CC'TCA_GC Cuts at: 2919 2919 Size: 5740 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3931 3965 1903 Size: 34 1994 34 3678 Fragments arranged by size: 3678 1994 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4351 136 Size: 107 122 71 631 605 2679 1525 Fragments arranged by size: 2679 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 2825 3801 4957 1790 Size: 1035 976 1156 2573 Fragments arranged by size: 2573 1156 1035 976 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2704 3471 4429 Size: 416 53 83 399 24 1287 767 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1287 958 767 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 2102 2878 4400 1239 Size: 813 50 776 1522 2579 Fragments arranged by size: 2579 1522 813 776 50 Bpu10I CC'TnA_GC Cuts at: 2784 2919 2784 Size: 135 5605 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4403 882 Size: 617 2904 2219 Fragments arranged by size: 2904 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3547 3929 4564 Size: 53 83 186 936 522 1492 382 635 Cuts at: 4564 275 Size: 1451 Fragments arranged by size: 1492 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3024 3054 1178 Size: 30 165 30 1621 30 3864 Fragments arranged by size: 3864 1621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2936 3048 1186 Size: 1000 750 112 3878 Fragments arranged by size: 3878 1000 750 112 BseYI C'CCAG_C Cuts at: 2190 3018 4943 5439 2190 Size: 828 1925 496 2491 Fragments arranged by size: 2491 1925 828 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2727 3190 3951 4979 1540 Size: 275 186 726 463 761 1028 2301 Fragments arranged by size: 2301 1028 761 726 463 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 2637 2790 2892 3801 3886 4957 2256 Size: 381 153 102 909 85 1071 3039 Fragments arranged by size: 3039 1071 909 381 153 102 85 BsiWI C'GTAC_G Cuts at: 2275 2275 Size: 5740 BsmI GAATG_Cn' Cuts at: 2793 3251 3344 2793 Size: 458 93 5189 Fragments arranged by size: 5189 458 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2569 2569 Size: 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2256 2637 2790 2825 2892 2902 Size: 399 433 33 381 153 35 67 10 Cuts at: 2902 3059 3801 3886 4957 1391 Size: 157 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 381 157 153 85 67 35 33 10 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 2457 3629 5359 2457 Size: 1172 1730 2838 Fragments arranged by size: 2838 1730 1172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3095 844 Size: 2251 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 2733 2842 4235 4409 65 Size: 1183 1149 336 109 1393 174 1396 Fragments arranged by size: 1396 1393 1183 1149 336 174 109 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 2772 96 Size: 1911 765 3064 Fragments arranged by size: 3064 1911 765 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 2841 794 Size: 2047 3693 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 2743 1184 Size: 1559 4181 BstYI r'GATC_y Cuts at: 1972 2154 2664 3070 3437 3822 3839 5291 5377 Size: 182 510 406 367 385 17 1452 86 Cuts at: 5377 5389 5735 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 510 406 385 367 346 182 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2066 513 Size: 552 1001 4187 Fragments arranged by size: 4187 1001 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2723 2726 4747 809 Size: 507 1407 3 2021 1802 Fragments arranged by size: 2021 1802 1407 507 3 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 3187 4076 Size: 54 1425 50 275 18 261 1096 889 Cuts at: 4076 5705 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1096 889 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 2204 2228 2543 2642 3670 1353 Size: 791 60 24 315 99 1028 3423 Fragments arranged by size: 3423 1028 791 315 99 60 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2254 2788 2890 2254 Size: 534 102 5104 Fragments arranged by size: 5104 534 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2102 2878 3803 4400 5187 5455 Size: 393 420 50 776 925 597 787 268 Cuts at: 5455 1239 Size: 1524 Fragments arranged by size: 1524 925 787 776 597 420 393 268 50 EcoNI CCTnn'n_nnAGG Cuts at: 2829 2829 Size: 5740 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2959 2991 4381 4413 4455 Size: 32 135 32 1387 32 1390 32 42 Cuts at: 4455 4487 1373 Size: 32 2626 Fragments arranged by size: 2626 1390 1387 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4854 5038 1305 Size: 3549 184 2007 Fragments arranged by size: 3549 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 10 Size: 54 1425 343 261 3657 Fragments arranged by size: 3657 1425 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2654 2986 3698 4057 4216 518 Size: 648 1488 332 712 359 159 2042 Fragments arranged by size: 2042 1488 712 648 359 332 159 MspA1I CmG'CkG Cuts at: 1606 3156 3833 4981 5226 1606 Size: 1550 677 1148 245 2120 Fragments arranged by size: 2120 1550 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2066 513 Size: 552 1001 4187 Fragments arranged by size: 4187 1001 552 NdeI CA'TA_TG Cuts at: 387 2944 387 Size: 2557 3183 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 3098 4643 1132 Size: 97 1869 1545 2229 Fragments arranged by size: 2229 1869 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 2754 1108 Size: 1646 4094 PfoI T'CCnGG_A Cuts at: 2378 2378 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 2168 3092 830 Size: 1338 924 3478 Fragments arranged by size: 3478 1338 924 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 SacI G_AGCT'C Cuts at: 2256 2790 2892 2256 Size: 534 102 5104 Fragments arranged by size: 5104 534 102 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SapI GCTCTTCn'nnn_ Cuts at: 2228 2642 2228 Size: 414 5326 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 2164 2800 3088 4223 4904 5095 826 Size: 219 1119 636 288 1135 681 191 1471 Fragments arranged by size: 1471 1135 1119 681 636 288 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3844 4745 5007 820 Size: 197 926 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2066 3179 513 Size: 552 1001 1113 3074 Fragments arranged by size: 3074 1113 1001 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2644 3767 3784 3937 4122 4567 Size: 1255 624 440 1123 17 153 185 445 Cuts at: 4567 325 Size: 1498 Fragments arranged by size: 1498 1255 1123 624 445 440 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2518 2772 Size: 274 80 33 51 1443 30 511 254 Cuts at: 2772 3986 5698 96 Size: 1214 1712 138 Fragments arranged by size: 1712 1443 1214 511 274 254 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 2723 1316 Size: 1407 4333 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI