Restriction map for
pFN21AE1136
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I TspRI TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 1000 GAATTC [GA]^AATT[CT]
AfaI 48 GTAC GT^AC
AlwNI 11 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1000 GAATTC [GA]^AATT[CT]
AspHI 273 GAGCAC G[AT]GC[AT]^C
AspI 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 787 CCCGAG C^[CT]CG[GA]G
AvaII 476 GGACC G^G[AT]CC
BalI 101 TGGCCA TGG^CCA
BamHI 305 GGATCC G^GATCC
BbsI 523 GAAGAC GAAGAC(2/6)
BbvCI 732 CCTCAGC CCTCAGC(-5/-2)
BglI 26 GCCGCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 523 GAAGAC GAAGAC(2/6)
BsgI 166 GTGCAG GTGCAG(16/14)
BsiHKAI 273 GAGCAC G[AT]GC[AT]^C
BsoBI 787 CCCGAG C^[CT]CG[GA]G
BspMI 713 GCAGGT ACCTGC(4/8)
BsrBI 506 CCGCTC CCGCTC(-3/-3)
BsrI 264 ACTGG ACTGG(1/-1)
BssSI 862 CTCGTG CACGAG(-5/-1)
BstXI 665 CCATCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 305 GGATCC [GA]^GATC[CT]
CfoI 565 GCGC GCG^C
EcoNI 426 CCTAGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1000 GAATTC G^AATTC
HaeII 564 GGCGCT [GA]GCGC^[CT]
HhaI 565 GCGC GCG^C
HinP1I 565 GCGC G^CGC
HindIII 74 AAGCTT A^AGCTT
MaeIII 118 GTGAC ^GT[ACGT]AC
MflI 305 GGATCC [GA]^GATC[CT]
MluNI 101 TGGCCA TGG^CCA
MscI 101 TGGCCA TGG^CCA
MslI 666 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 973 CAGCTG C[AC]G^C[GT]G
NcoI 821 CCATGG C^CATGG
PflFI 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 752 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 172 GACTC GAGTC(4/5)
PshAI 477 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 976 CTGCAG CTGCA^G
PvuII 973 CAGCTG CAG^CTG
RsaI 48 GTAC GT^AC
SapI 870 GAAGAGC GCTCTTC(1/4)
ScaI 47 AGTACT AGT^ACT
TfiI 399 GATTC G^A[AT]TC
Tsp45I 118 GTGAC ^GT[GC]AC
Tsp509I 1001 AATT ^AATT
Tth111I 526 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 752 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 305 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BcnI 110 CCGGG CC^[GC]GG
369 CCGGG
BfaI 91 CTAG C^TAG
427 CTAG
BseRI 509 CTCCTC GAGGAG(10/8)
652 CTCCTC
BsmAI 621 GAGAC GTCTC(1/5)
931 GAGAC
Cac8I 332 GCCTGC GC[ACGT]^[ACGT]GC
336 GCTGGC
DraII 279 AGGGCCC [GA]G^G[ACGT]CC[CT]
628 GGGGCCC
EcoO109I 279 AGGGCCC [GA]G^G[ACGT]CC[CT]
628 GGGGCCC
EcoT14I 743 CCTTGG C^C[AT][AT]GG
821 CCATGG
HapII 110 CCGG C^CGG
369 CCGG
HinfI 172 GACTC G^A[ACGT]TC
399 GATTC
HpaII 110 CCGG C^CGG
369 CCGG
MaeI 91 CTAG C^TAG
427 CTAG
MaeII 482 ACGT A^CGT
548 ACGT
MspI 110 CCGG C^CGG
369 CCGG
NciI 110 CCGGG CC^[GC]GG
369 CCGGG
SfcI 51 CTACAG C^T[GA][CT]AG
976 CTGCAG
SmlI 902 CTCAAG C^T[CT][GA]AG
917 CTCAAG
StyI 743 CCTTGG C^C[AT][AT]GG
821 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI ApaI BanII
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrDI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HphI ItaI Ksp632I MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI