(Circular) (Six-base) MAPSORT of: pFN21AE1136.seq Check: 1073 from: 1 to: 5776 pFN21AE1136 5776 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2709 2709 Size: 5776 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2410 3839 5223 5491 1632 Size: 465 313 1429 1384 268 1917 Fragments arranged by size: 1917 1429 1384 465 313 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2021 3519 5091 2021 Size: 1498 1572 2706 Fragments arranged by size: 2706 1572 1498 ApaI G_GGCC'C Cuts at: 2289 2638 3025 2289 Size: 349 387 5040 Fragments arranged by size: 5040 387 349 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3005 3085 3311 3463 5572 1493 Size: 1512 80 226 152 2109 1697 Fragments arranged by size: 2109 1697 1512 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2792 3101 1870 Size: 73 849 309 4545 Fragments arranged by size: 4545 849 309 73 BamHI G'GATC_C Cuts at: 2310 3106 3473 2310 Size: 796 367 4613 Fragments arranged by size: 4613 796 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2289 2638 3025 3041 3095 1391 Size: 898 349 387 16 54 4072 Fragments arranged by size: 4072 898 387 349 54 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 928 Size: 971 636 4169 Fragments arranged by size: 4169 971 636 BbvCI CC'TCA_GC Cuts at: 2738 2738 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 4387 136 Size: 107 122 71 631 605 365 2350 1525 Fragments arranged by size: 2350 1525 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2289 2638 3025 3837 4993 1790 Size: 499 349 387 812 1156 2573 Fragments arranged by size: 2573 1156 812 499 387 349 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4436 1239 Size: 3197 2579 Bpu10I CC'TnA_GC Cuts at: 2738 2738 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2892 2907 3901 4766 5064 2892 Size: 15 994 865 298 3604 Fragments arranged by size: 3604 994 865 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5551 Fragments arranged by size: 5551 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2505 2648 1186 Size: 1319 143 4314 Fragments arranged by size: 4314 1319 143 BseYI C'CCAG_C Cuts at: 2763 4979 5475 2763 Size: 2216 496 3064 Fragments arranged by size: 3064 2216 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 1163 Size: 573 456 4747 Fragments arranged by size: 4747 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2282 3837 3922 4993 2282 Size: 1555 85 1071 3065 Fragments arranged by size: 3065 1555 1071 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2282 2289 2638 3025 3041 3095 3837 Size: 399 492 7 349 387 16 54 742 Cuts at: 3837 3922 4993 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 387 349 85 54 16 7 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2709 3131 844 Size: 1865 422 3489 Fragments arranged by size: 3489 1865 422 BsrBI CCG'CTC Cuts at: 1394 1823 2513 3663 1394 Size: 429 690 1150 3507 Fragments arranged by size: 3507 1150 690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2041 2530 2664 4271 4445 65 Size: 1183 793 489 134 1607 174 1396 Fragments arranged by size: 1607 1396 1183 793 489 174 134 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2867 3836 4848 2867 Size: 969 1012 3795 Fragments arranged by size: 3795 1012 969 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2677 2677 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2310 3106 3473 3858 3875 5327 5413 5425 Size: 338 796 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 796 385 367 346 338 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 2826 3017 513 Size: 552 939 414 408 191 3272 Fragments arranged by size: 3272 939 552 414 408 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2415 3032 Size: 54 1425 50 275 18 276 309 617 Cuts at: 3032 3223 4112 5741 8 Size: 191 889 1629 43 Fragments arranged by size: 1629 1425 889 617 309 276 275 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 2564 2618 2870 2928 3706 1353 Size: 866 345 54 252 58 778 3423 Fragments arranged by size: 3423 866 778 345 252 58 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2734 4363 4737 4883 387 Size: 1064 138 1145 1629 374 146 1280 Fragments arranged by size: 1629 1280 1145 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2410 3839 4436 5223 5491 1239 Size: 393 465 313 1429 597 787 268 1524 Fragments arranged by size: 1524 1429 787 597 465 393 313 268 EcoNI CCTnn'n_nnAGG Cuts at: 2435 2435 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2285 2634 2285 Size: 349 5427 EcoRI G'AATT_C Cuts at: 3005 3085 3463 3005 Size: 80 378 5318 Fragments arranged by size: 5318 378 80 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2573 4923 1678 Size: 895 2350 2531 Fragments arranged by size: 2531 2350 895 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2395 2427 4417 4449 4491 Size: 32 135 32 823 32 1990 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 1990 823 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 2079 748 Size: 1331 4445 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 4890 5074 1305 Size: 900 2685 184 2007 Fragments arranged by size: 2685 2007 900 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 10 Size: 54 1425 343 276 3678 Fragments arranged by size: 3678 1425 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2675 3734 4093 4252 518 Size: 648 1509 1059 359 159 2042 Fragments arranged by size: 2042 1509 1059 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2980 3192 3869 5017 5262 1606 Size: 1374 212 677 1148 245 2120 Fragments arranged by size: 2120 1374 1148 677 245 212 NcoI C'CATG_G Cuts at: 513 1065 2004 2826 513 Size: 552 939 822 3463 Fragments arranged by size: 3463 939 822 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2763 1108 Size: 1655 4121 PfoI T'CCnGG_A Cuts at: 2436 2436 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2486 1613 Size: 873 4903 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2285 2634 3021 2285 Size: 349 387 5040 Fragments arranged by size: 5040 387 349 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2885 2917 2885 Size: 32 5744 PstI C_TGCA'G Cuts at: 830 2985 3128 830 Size: 2155 143 3478 Fragments arranged by size: 3478 2155 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2980 2980 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2870 2870 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2054 4024 5736 2054 Size: 1970 1712 2094 Fragments arranged by size: 2094 1970 1712 SfcI C'TryA_G Cuts at: 826 1045 2056 2981 3124 4259 4940 5131 826 Size: 219 1011 925 143 1135 681 191 1471 Fragments arranged by size: 1471 1135 1011 925 681 219 191 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2907 2922 3880 4781 5043 820 Size: 197 926 964 15 958 901 262 1553 Fragments arranged by size: 1553 964 958 926 901 262 197 15 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2748 2826 3215 513 Size: 552 939 744 78 389 3074 Fragments arranged by size: 3074 939 744 552 389 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 2665 2755 3803 3820 3973 4158 Size: 1255 633 452 90 1048 17 153 185 Cuts at: 4158 4603 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1048 633 452 445 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 4022 5734 Size: 274 80 33 51 1443 75 1970 1712 Cuts at: 5734 96 Size: 138 Fragments arranged by size: 1970 1712 1443 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 5736 5768 2283 Size: 32 3421 32 2291 Fragments arranged by size: 3421 2291 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2534 1316 Size: 1218 4558 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI