################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 291 GTCTAC GT^[AC][GT]AC AgeI 485 ACCGGT A^CCGGT AlwI 563 GGATC GGATC(4/5) AspI 285 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 184 CCCGGG C^[CT]CG[GA]G BcgI 353 GCACGCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 455 AGATCT A^GATCT BlpI 883 GCTCAGC GC^T[ACGT]AGC Bpu1102I 883 GCTCAGC GC^T[ACGT]AGC BsaI 312 GGTCTC GGTCTC(1/5) BsaWI 485 ACCGGT [AT]^CCGG[AT] BsgI 81 CTGCAC GTGCAG(16/14) BsoBI 184 CCCGGG C^[CT]CG[GA]G BsrDI 1064 GCAATG GCAATG(2/0) BsrFI 485 ACCGGT [GA]^CCGG[CT] CelII 883 GCTCAGC GC^T[ACGT]AGC Cfr10I 485 ACCGGT [GA]^CCGG[CT] Cfr13I 1211 GGCCC G^G[ACGT]CC DraIII 169 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 951 GATATC GAT^ATC HgaI 393 GACGC GACGC(5/10) HindIII 1201 AAGCTT A^AGCTT MslI 895 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 78 CAGCTG C[AC]G^C[GT]G PflFI 285 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 678 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 485 ACCGGT A^CCGGT PvuII 78 CAGCTG CAG^CTG Sau96I 1211 GGCCC G^G[ACGT]CC SmaI 184 CCCGGG CCC^GGG TaqI 1162 TCGA T^CGA Tsp45I 583 GTGAC ^GT[GC]AC Tth111I 285 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1162 TCGA T^CGA Van91I 678 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 184 CCCGGG C^CCGGG XmaI 184 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 75 CCGC CCGC(-3/-1) 553 GCGG AspHI 136 GAGCAC G[AT]GC[AT]^C 592 GTGCTC BanI 60 GGTGCC G^G[CT][GA]CC 893 GGCACC BcnI 184 CCCGG CC^[GC]GG 185 CCGGG BpmI 370 CTCCAG CTGGAG(16/14) 443 CTCCAG BsiHKAI 136 GAGCAC G[AT]GC[AT]^C 592 GTGCTC BsmAI 1027 GAGAC GTCTC(1/5) 313 GTCTC BsmFI 617 GGGAC GGGAC(10/14) 799 GTCCC BstYI 455 AGATCT [GA]^GATC[CT] 563 GGATCT DsaI 317 CCATGG C^C[GA][CT]GG 1136 CCATGG HapII 185 CCGG C^CGG 486 CCGG HpaII 185 CCGG C^CGG 486 CCGG HphI 777 GGTGA GGTGA(8/7) 377 TCACC MflI 455 AGATCT [GA]^GATC[CT] 563 GGATCT MspI 185 CCGG C^CGG 486 CCGG NciI 184 CCCGG CC^[GC]GG 185 CCGGG NcoI 317 CCATGG C^CATGG 1136 CCATGG NspI 322 GCATGT [GA]CATG^[CT] 1037 GCATGT TfiI 219 GATTC G^A[AT]TC 699 GATTC XcmI 299 CCAACTACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 372 CCAGTTCACCACTGG XhoII 455 AGATCT [GA]^GATC[CT] 563 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AluI BalI BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI StyI Tru9I TseI Tsp509I TspRI