(Circular) (Six-base) MAPSORT of: pFN21AB8826.seq Check: 9664 from: 1 to: 5950 pFN21AB8826 5950 bp With 182 enzymes: SgfI * March 28, 2012 12:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2297 3293 1937 Size: 360 996 4594 Fragments arranged by size: 4594 996 360 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2955 4013 5397 5665 1632 Size: 1323 1058 1384 268 1917 Fragments arranged by size: 1917 1384 1323 1058 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 2490 4781 2490 Size: 2291 3659 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2441 1184 Size: 1257 4693 AlwNI CAG_nnn'CTG Cuts at: 3693 5265 3693 Size: 1572 4378 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 2189 3275 1870 Size: 73 246 1086 4545 Fragments arranged by size: 4545 1086 246 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2898 3271 4726 618 Size: 325 217 627 278 833 373 1455 1842 Fragments arranged by size: 1842 1455 833 627 373 325 278 217 BanII G_rGCy'C Cuts at: 1391 3269 1391 Size: 1878 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4979 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2347 2381 4141 4175 1903 Size: 34 410 34 1760 34 3678 Fragments arranged by size: 3678 1760 410 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2460 5945 2460 Size: 3485 2465 BlpI GC'TnA_GC Cuts at: 2889 3367 2889 Size: 478 5472 Bme1580I G_kGCm'C Cuts at: 1790 2070 4011 5167 1790 Size: 280 1941 1156 2573 Fragments arranged by size: 2573 1941 1156 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2629 3681 4639 Size: 416 53 83 399 24 1212 1052 958 Cuts at: 4639 442 Size: 1753 Fragments arranged by size: 1753 1212 1052 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2433 4610 1239 Size: 1121 73 2177 2579 Fragments arranged by size: 2579 2177 1121 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2323 4613 882 Size: 617 824 2290 2219 Fragments arranged by size: 2290 2219 824 617 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3757 4139 4774 275 Size: 53 83 186 936 2224 382 635 1451 Fragments arranged by size: 2224 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 4383 4781 5055 5202 5613 2490 Size: 1893 398 274 147 411 2827 Fragments arranged by size: 2827 1893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5725 Fragments arranged by size: 5725 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2462 2641 2800 1186 Size: 967 309 179 159 4336 Fragments arranged by size: 4336 967 309 179 159 BseYI C'CCAG_C Cuts at: 2401 5153 5649 2401 Size: 2752 496 2702 Fragments arranged by size: 2752 2702 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 1163 Size: 573 335 5042 Fragments arranged by size: 5042 573 335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2601 4011 4096 5167 2145 Size: 456 1410 85 1071 2928 Fragments arranged by size: 2928 1410 1071 456 85 BsmI GAATG_Cn' Cuts at: 3461 3554 3461 Size: 93 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2145 2601 3269 4011 4096 5167 Size: 399 280 75 456 668 742 85 1071 Cuts at: 5167 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 668 456 399 280 85 75 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3837 1394 Size: 429 2014 3507 Fragments arranged by size: 3507 2014 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3076 4445 4619 65 Size: 1183 1828 1369 174 1396 Fragments arranged by size: 1828 1396 1369 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2490 4594 4781 1700 Size: 110 680 2104 187 2869 Fragments arranged by size: 2869 2104 680 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstYI r'GATC_y Cuts at: 1972 2460 2568 3280 3647 4032 4049 5501 5587 Size: 488 108 712 367 385 17 1452 86 Cuts at: 5587 5599 5945 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 712 488 385 367 346 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2322 3141 513 Size: 552 939 318 819 3322 Fragments arranged by size: 3322 939 819 552 318 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 2664 3466 4260 4280 2664 Size: 802 794 20 4334 Fragments arranged by size: 4334 802 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DraIII CAC_nnn'GTG Cuts at: 2179 2179 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2773 3102 Size: 54 1425 50 275 18 369 574 329 Cuts at: 3102 3144 3397 4286 5915 8 Size: 42 253 889 1629 43 Fragments arranged by size: 1629 1425 889 574 369 329 275 253 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3880 1353 Size: 2527 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2433 2955 4013 4610 5397 5665 Size: 393 728 73 522 1058 597 787 268 Cuts at: 5665 1239 Size: 1524 Fragments arranged by size: 1524 1058 787 728 597 522 393 268 73 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 EcoRV GAT'ATC Cuts at: 2958 2958 Size: 5950 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 5097 1678 Size: 3419 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373 Size: 32 135 32 3019 32 42 32 2626 Fragments arranged by size: 3019 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 3206 748 Size: 2458 3492 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2129 2640 5064 5248 1305 Size: 824 511 2424 184 2007 Fragments arranged by size: 2424 2007 824 511 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 3104 3146 10 Size: 54 1425 343 369 903 42 2814 Fragments arranged by size: 2814 1425 903 369 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2904 3908 4267 4426 518 Size: 648 1738 1004 359 159 2042 Fragments arranged by size: 2042 1738 1004 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 3366 4043 5191 5436 1606 Size: 479 1281 677 1148 245 2120 Fragments arranged by size: 2120 1281 1148 677 479 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2322 3141 513 Size: 552 939 318 819 3322 Fragments arranged by size: 3322 939 819 552 318 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2331 3046 3308 4853 1132 Size: 97 1102 715 262 1545 2229 Fragments arranged by size: 2229 1545 1102 715 262 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 2689 1108 Size: 1581 4369 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3003 3520 3003 Size: 517 5433 PstI C_TGCA'G Cuts at: 830 3302 830 Size: 2472 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2085 2085 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 3298 4433 5114 5305 826 Size: 219 2253 1135 681 191 1471 Fragments arranged by size: 2253 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 2191 3277 1872 Size: 319 1086 4545 Fragments arranged by size: 4545 1086 319 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 3141 3195 3389 513 Size: 552 939 318 819 54 194 3074 Fragments arranged by size: 3074 939 819 552 318 194 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3977 3994 4147 4332 4777 325 Size: 1255 2397 17 153 185 445 1498 Fragments arranged by size: 2397 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4196 5908 96 Size: 274 80 33 51 1443 2219 1712 138 Fragments arranged by size: 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 2293 1316 Size: 977 4973 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2384 2311 Size: 73 5877 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 2189 3275 1870 Size: 319 1086 4545 Fragments arranged by size: 4545 1086 319 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI