(Linear) (Six-base) MAPSORT of: KIBB8826.seq Check: 5903 from: 1 to: 1245 KIBB8826.orf With 182 enzymes: SgfI * March 22, 2012 15:44 .. AccI GT'mk_AC Cuts at: 0 292 1245 Size: 292 953 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 950 1245 Size: 950 295 AgeI A'CCGG_T Cuts at: 0 485 1245 Size: 485 760 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 436 1245 Size: 436 809 AvaI C'yCGr_G Cuts at: 0 184 1245 Size: 184 1061 BanI G'GyrC_C Cuts at: 0 60 893 1245 Size: 60 833 352 Fragments arranged by size: 833 352 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 342 376 1245 Size: 342 34 869 Fragments arranged by size: 869 342 34 BglII A'GATC_T Cuts at: 0 455 1245 Size: 455 790 BlpI GC'TnA_GC Cuts at: 0 884 1245 Size: 884 361 Bme1580I G_kGCm'C Cuts at: 0 65 1245 Size: 65 1180 BmrI ACTGGGnnnn_n' Cuts at: 0 624 1245 Size: 624 621 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 428 1245 Size: 355 73 817 Fragments arranged by size: 817 355 73 BsaI GGTCTCn'nnnn_ Cuts at: 0 318 1245 Size: 318 927 BsaWI w'CCGG_w Cuts at: 0 485 1245 Size: 485 760 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 148 457 636 795 1245 Size: 148 309 179 159 450 Fragments arranged by size: 450 309 179 159 148 BseYI C'CCAG_C Cuts at: 0 396 1245 Size: 396 849 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 66 1245 Size: 66 1179 BsiHKAI G_wGCw'C Cuts at: 0 140 596 1245 Size: 140 456 649 Fragments arranged by size: 649 456 140 Bsp1286I G_dGCh'C Cuts at: 0 65 140 596 1245 Size: 65 75 456 649 Fragments arranged by size: 649 456 75 65 BsrDI GCAATG_nn' Cuts at: 0 1071 1245 Size: 1071 174 BsrFI r'CCGG_y Cuts at: 0 485 1245 Size: 485 760 BstYI r'GATC_y Cuts at: 0 455 563 1245 Size: 455 108 682 Fragments arranged by size: 682 455 108 BtgI C'CryG_G Cuts at: 0 317 1136 1245 Size: 317 819 109 Fragments arranged by size: 819 317 109 BtsI GCAGTG_nn' Cuts at: 0 659 1245 Size: 659 586 DraIII CAC_nnn'GTG Cuts at: 0 174 1245 Size: 174 1071 EaeI y'GGCC_r Cuts at: 0 194 768 1097 1139 1245 Size: 194 574 329 42 106 Fragments arranged by size: 574 329 194 106 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 428 950 1245 Size: 355 73 522 295 Fragments arranged by size: 522 355 295 73 EcoRV GAT'ATC Cuts at: 0 953 1245 Size: 953 292 HindIII A'AGCT_T Cuts at: 0 1201 1245 Size: 1201 44 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 124 635 1245 Size: 124 511 610 Fragments arranged by size: 610 511 124 MscI TGG'CCA Cuts at: 0 196 1099 1141 1245 Size: 196 903 42 104 Fragments arranged by size: 903 196 104 42 MslI CAynn'nnrTG Cuts at: 0 899 1245 Size: 899 346 MspA1I CmG'CkG Cuts at: 0 80 1245 Size: 80 1165 NcoI C'CATG_G Cuts at: 0 317 1136 1245 Size: 317 819 109 Fragments arranged by size: 819 317 109 NspI r_CATG'y Cuts at: 0 326 1041 1245 Size: 326 715 204 Fragments arranged by size: 715 326 204 PflMI CCAn_nnn'nTGG Cuts at: 0 684 1245 Size: 684 561 PsiI TTA'TAA Cuts at: 0 998 1245 Size: 998 247 PvuII CAG'CTG Cuts at: 0 80 1245 Size: 80 1165 SmaI CCC'GGG Cuts at: 0 186 1245 Size: 186 1059 StyI C'CwwG_G Cuts at: 0 317 1136 1190 1245 Size: 317 819 54 55 Fragments arranged by size: 819 317 55 54 Tth111I GACn'n_nGTC Cuts at: 0 288 1245 Size: 288 957 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 306 379 1245 Size: 306 73 866 Fragments arranged by size: 866 306 73 XmaI C'CCGG_G Cuts at: 0 184 1245 Size: 184 1061 Enzymes that do cut: AccI AcuI AgeI AlfI AvaI BanI BcgI BglII BlpI Bme1580I BmrI BpmI BsaI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BstYI BtgI BtsI DraIII EaeI Eco57MI EcoRV HindIII MmeI MscI MslI MspA1I NcoI NspI PflMI PsiI PvuII SmaI StyI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XhoI XmnI ZraI