################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BanII BbeI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 829 GACGTC GACGT^C AccI 976 GTAGAC GT^[AC][GT]AC AcyI 829 GACGTC G[GA]^CG[CT]C AfaI 897 GTAC GT^AC AhdI 95 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 279 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 95 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 751 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 490 TGGCCA TGG^CCA BamHI 923 GGATCC G^GATCC BanI 889 GGCACC G^G[CT][GA]CC BbrPI 577 CACGTG CAC^GTG BsaAI 577 CACGTG [CT]AC^GT[GA] BsaHI 829 GACGTC G[GA]^CG[CT]C BsiYI 705 CCACTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 705 CCACTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 159 GTCCC GGGAC(10/14) BsmI 283 GCATTC GAATGC(1/-1) BsrDI 456 CATTGC GCAATG(2/0) BstYI 923 GGATCC [GA]^GATC[CT] DsaI 919 CCATGG C^C[GA][CT]GG Eam1105I 95 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 124 CTCTTC CTCTTC(1/4) EcoRI 67 GAATTC G^AATTC EcoRV 960 GATATC GAT^ATC Hin1I 829 GACGTC G[GA]^CG[CT]C HphI 130 TCACC GGTGA(8/7) Ksp632I 124 CTCTTC CTCTTC(1/4) MflI 923 GGATCC [GA]^GATC[CT] MluNI 490 TGGCCA TGG^CCA MscI 490 TGGCCA TGG^CCA MseI 809 TTAA T^TAA NcoI 919 CCATGG C^CATGG PflFI 751 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 193 GAGTC GAGTC(4/5) PmaCI 577 CACGTG CAC^GTG PmlI 577 CACGTG CAC^GTG RsaI 897 GTAC GT^AC SexAI 752 ACCAGGT A^CC[AT]GGT SfcI 584 CTGTAG C^T[GA][CT]AG SspI 650 AATATT AAT^ATT Tru9I 809 TTAA T^TAA Tth111I 751 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 923 GGATCC [GA]^GATC[CT] XmnI 279 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 67 GAATTC [GA]^AATT[CT] 225 AAATTC AlwI 924 GATCC GGATC(4/5) 923 GGATC ApoI 67 GAATTC [GA]^AATT[CT] 225 AAATTC AspHI 111 GAGCAC G[AT]GC[AT]^C 318 GAGCAC BbsI 103 GTCTTC GAAGAC(2/6) 270 GTCTTC BfaI 40 CTAG C^TAG 625 CTAG BmyI 111 GAGCAC G[AGT]GC[ACT]^C 318 GAGCAC BpuAI 103 GTCTTC GAAGAC(2/6) 270 GTCTTC BsaI 333 GGTCTC GGTCTC(1/5) 553 GGTCTC BsiHKAI 111 GAGCAC G[AT]GC[AT]^C 318 GAGCAC BsmAI 334 GTCTC GTCTC(1/5) 554 GTCTC Bsp1286I 111 GAGCAC G[AGT]GC[ACT]^C 318 GAGCAC BsrI 11 CCAGT ACTGG(1/-1) 236 CCAGT BstF5I 714 GGATG GGATG(2/0) 940 GGATG DpnII 661 GATC ^GATC 924 GATC DrdI 201 GACAGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 439 GACCCCAAAGTC EaeI 490 TGGCCA [CT]^GGCC[GA] 836 CGGCCA FokI 714 GGATG GGATG(9/13) 940 GGATG MaeI 40 CTAG C^TAG 625 CTAG MboI 661 GATC ^GATC 924 GATC NdeII 661 GATC ^GATC 924 GATC PpuMI 755 AGGTCCT [GA]G^G[AT]CC[CT] 875 GGGTCCT Sau3AI 661 GATC ^GATC 924 GATC Tsp45I 20 GTGAC ^GT[GC]AC 832 GTCAC XcmI 705 CCACTTCCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 927 CCAGCAGCACGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI AvaII BbvI BsaJI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HinfI ItaI MaeII MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI StyI TfiI TseI Tsp509I TspRI