################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 514 GTAC GT^AC AlwI 643 GGATC GGATC(4/5) Asp700I 242 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 666 GGTCC G^G[AT]CC AvrII 754 CCTAGG C^CTAGG BcnI 774 CCCGG CC^[GC]GG BfaI 755 CTAG C^TAG BlnI 754 CCTAGG C^CTAGG BpmI 159 CTCCAG CTGGAG(16/14) BsaBI 588 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 156 GGTCTC GGTCTC(1/5) BsmAI 157 GTCTC GTCTC(1/5) BsmI 817 GCATTC GAATGC(1/-1) BstNI 260 CCTGG CC^[AT]GG Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC Cfr13I 666 GGTCC G^G[ACGT]CC DraI 360 TTTAAA TTT^AAA DrdI 772 GACCCGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 633 CCATGG C^C[GA][CT]GG EarI 108 GAAGAG CTCTTC(1/4) EcoRII 260 CCTGG ^CC[AT]GG EcoRV 576 GATATC GAT^ATC HapII 775 CCGG C^CGG HindIII 677 AAGCTT A^AGCTT HpaII 775 CCGG C^CGG Ksp632I 108 GAAGAG CTCTTC(1/4) MaeI 755 CTAG C^TAG MamI 588 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 384 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 128 CAGCTG C[AC]G^C[GT]G MspI 775 CCGG C^CGG MvaI 260 CCTGG CC^[AT]GG NciI 774 CCCGG CC^[GC]GG NcoI 633 CCATGG C^CATGG NspI 284 GCATGT [GA]CATG^[CT] PvuII 128 CAGCTG CAG^CTG RsaI 514 GTAC GT^AC Sau96I 666 GGTCC G^G[ACGT]CC TaqI 821 TCGA T^CGA TfiI 213 GAATC G^A[AT]TC TthHB8I 821 TCGA T^CGA XmnI 242 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 154 GCGG CCGC(-3/-1) 765 GCGG AcsI 90 AAATTT [GA]^AATT[CT] 137 AAATTC ApoI 90 AAATTT [GA]^AATT[CT] 137 AAATTC BbvCI 125 CCTCAGC CCTCAGC(-5/-2) 303 CCTCAGC BglII 119 AGATCT A^GATCT 651 AGATCT BseRI 123 CTCCTC GAGGAG(10/8) 366 GAGGAG BsmFI 403 GGGAC GGGAC(10/14) 570 GTCCC BssKI 260 CCTGG ^CC[ACGT]GG 774 CCCGG BstF5I 831 CATCC GGATG(2/0) 447 GGATG BstYI 119 AGATCT [GA]^GATC[CT] 651 AGATCT FokI 831 CATCC GGATG(9/13) 447 GGATG HphI 105 GGTGA GGTGA(8/7) 390 GGTGA MaeIII 268 GTAAC ^GT[ACGT]AC 535 GTAAC MflI 119 AGATCT [GA]^GATC[CT] 651 AGATCT PleI 823 GACTC GAGTC(4/5) 750 GAGTC ScrFI 260 CCTGG CC^[ACGT]GG 774 CCCGG SfaNI 588 GATGC GCATC(5/9) 624 GATGC SmlI 322 CTCAAG C^T[CT][GA]AG 616 CTCAAG TspRI 83 CACTG CA[GC]TG[ACGT][ACGT]^ 144 CACTG XhoII 119 AGATCT [GA]^GATC[CT] 651 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI BbvI BsaJI BsrI DdeI DpnII EcoT14I Fnu4HI HinfI ItaI MboI MboII MnlI MseI NdeII NlaIII Sau3AI StyI Tru9I TseI Tsp509I