Restriction map for
pFN21AE1712
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 409 GTATAC GT^[AC][GT]AC
AfaI 472 GTAC GT^AC
Alw44I 566 GTGCAC G^TGCAC
ApaLI 566 GTGCAC G^TGCAC
AspHI 566 GTGCAC G[AT]GC[AT]^C
AvaI 579 CTCGAG C^[CT]CG[GA]G
AvaII 423 GGACC G^G[AT]CC
BbvI 167 GCTGC GCAGC(8/12)
BfaI 469 CTAG C^TAG
BmyI 566 GTGCAC G[AGT]GC[ACT]^C
BsaI 23 GGTCTC GGTCTC(1/5)
BsaJI 185 CCATGG C^C[ACGT][ACGT]GG
BsiHKAI 566 GTGCAC G[AT]GC[AT]^C
BsiYI 481 CCAACAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 481 CCAACAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 465 GGGAC GGGAC(10/14)
BsoBI 579 CTCGAG C^[CT]CG[GA]G
Bsp1286I 566 GTGCAC G[AGT]GC[ACT]^C
Bst1107I 409 GTATAC GTA^TAC
BstAPI 568 GCACTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 461 GGATG GGATG(2/0)
BstZ17I 409 GTATAC GTA^TAC
DraI 586 TTTAAA TTT^AAA
DsaI 185 CCATGG C^C[GA][CT]GG
EarI 78 GAAGAG CTCTTC(1/4)
EcoT14I 185 CCATGG C^C[AT][AT]GG
EcoT22I 146 ATGCAT ATGCA^T
Fnu4HI 167 GCTGC GC^[ACGT]GC
FokI 461 GGATG GGATG(9/13)
HaeIII 289 GGCC GG^CC
ItaI 167 GCTGC GC^[ACGT]GC
Ksp632I 78 GAAGAG CTCTTC(1/4)
MaeI 469 CTAG C^TAG
MspA1I 33 CAGCTG C[AC]G^C[GT]G
NcoI 185 CCATGG C^CATGG
NlaIV 511 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 146 ATGCAT ATGCA^T
NspI 632 GCATGC [GA]CATG^[CT]
PaeR7I 579 CTCGAG C^TCGAG
PflMI 481 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 33 CAGCTG CAG^CTG
RsaI 472 GTAC GT^AC
SfaNI 148 GCATC GCATC(5/9)
SfcI 179 CTGTAG C^T[GA][CT]AG
SmlI 579 CTCGAG C^T[CT][GA]AG
SpeI 468 ACTAGT A^CTAGT
SphI 632 GCATGC GCATG^C
StyI 185 CCATGG C^C[AT][AT]GG
TseI 167 GCTGC G^C[AT]GC
TspRI 499 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 481 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 579 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 49 GAATTT [GA]^AATT[CT]
263 AAATTC
AluI 34 AGCT AG^CT
583 AGCT
AlwI 69 GGATC GGATC(4/5)
490 GGATC
ApoI 49 GAATTT [GA]^AATT[CT]
263 AAATTC
BssKI 369 CCAGG ^CC[ACGT]GG
426 CCAGG
BstNI 369 CCAGG CC^[AT]GG
426 CCAGG
BstYI 69 GGATCT [GA]^GATC[CT]
490 GGATCT
Cac8I 394 GCCAGC GC[ACGT]^[ACGT]GC
632 GCATGC
Cfr13I 289 GGCCC G^G[ACGT]CC
423 GGACC
DpnII 70 GATC ^GATC
491 GATC
EcoRII 369 CCAGG ^CC[AT]GG
426 CCAGG
HinfI 9 GATTC G^A[ACGT]TC
191 GAATC
HphI 444 GGTGA GGTGA(8/7)
523 TCACC
MaeIII 281 GTTAC ^GT[ACGT]AC
314 GTTAC
MboI 70 GATC ^GATC
491 GATC
MflI 69 GGATCT [GA]^GATC[CT]
490 GGATCT
MseI 437 TTAA T^TAA
587 TTAA
MslI 201 CATTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
627 CATCTGCATG
MvaI 369 CCAGG CC^[AT]GG
426 CCAGG
MwoI 568 GCACTCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
584 GCTTTAAAGGC
NdeII 70 GATC ^GATC
491 GATC
Sau3AI 70 GATC ^GATC
491 GATC
Sau96I 289 GGCCC G^G[ACGT]CC
423 GGACC
ScrFI 369 CCAGG CC^[ACGT]GG
426 CCAGG
TaqI 27 TCGA T^CGA
580 TCGA
TfiI 9 GATTC G^A[AT]TC
191 GAATC
Tru9I 437 TTAA T^TAA
587 TTAA
TthHB8I 27 TCGA T^CGA
580 TCGA
XhoII 69 GGATCT [GA]^GATC[CT]
490 GGATCT
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
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BsmAI BsrI MboII MnlI NlaIII
Tsp509I