################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 548 GCGG CCGC(-3/-1) AcsI 386 AAATTT [GA]^AATT[CT] AcyI 163 GGCGTC G[GA]^CG[CT]C ApoI 386 AAATTT [GA]^AATT[CT] BbrPI 675 CACGTG CAC^GTG BbsI 72 GTCTTC GAAGAC(2/6) BciVI 634 GGATAC GTATCC(6/5) BpuAI 72 GTCTTC GAAGAC(2/6) BsaHI 163 GGCGTC G[GA]^CG[CT]C BseRI 573 CTCCTC GAGGAG(10/8) BsiYI 580 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 580 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspMI 177 GCAGGT ACCTGC(4/8) BsrDI 189 CATTGC GCAATG(2/0) BssKI 586 CCTGG ^CC[ACGT]GG BssSI 4 CACGAG CACGAG(-5/-1) BstNI 586 CCTGG CC^[AT]GG DdeI 533 CTCAG C^T[ACGT]AG DraI 423 TTTAAA TTT^AAA EcoRII 586 CCTGG ^CC[AT]GG HaeIII 589 GGCC GG^CC Hin1I 163 GGCGTC G[GA]^CG[CT]C HinfI 351 GATTC G^A[ACGT]TC HphI 111 GGTGA GGTGA(8/7) MaeIII 371 GTTAC ^GT[ACGT]AC MfeI 345 CAATTG C^AATTG MslI 189 CATTGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 546 CAGCGG C[AC]G^C[GT]G MunI 345 CAATTG C^AATTG MvaI 586 CCTGG CC^[AT]GG NspI 94 GCATGC [GA]CATG^[CT] PmaCI 675 CACGTG CAC^GTG PmlI 675 CACGTG CAC^GTG PstI 669 CTGCAG CTGCA^G ScrFI 586 CCTGG CC^[ACGT]GG SfcI 669 CTGCAG C^T[GA][CT]AG SmlI 489 CTTGAG C^T[CT][GA]AG SphI 94 GCATGC GCATG^C SspI 281 AATATT AAT^ATT TfiI 351 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 608 GATCC GGATC(4/5) 713 GATCC BmyI 230 GGGCAC G[AGT]GC[ACT]^C 266 GTGCCC BsaAI 637 TACGTG [CT]AC^GT[GA] 675 CACGTG BsmAI 379 GTCTC GTCTC(1/5) 531 GTCTC Bsp1286I 230 GGGCAC G[AGT]GC[ACT]^C 266 GTGCCC BstYI 607 AGATCC [GA]^GATC[CT] 712 AGATCC DpnII 608 GATC ^GATC 713 GATC EarI 26 CTCTTC CTCTTC(1/4) 255 GAAGAG HgaI 551 GACGC GACGC(5/10) 164 GCGTC Ksp632I 26 CTCTTC CTCTTC(1/4) 255 GAAGAG MaeII 638 ACGT A^CGT 676 ACGT MboI 608 GATC ^GATC 713 GATC MflI 607 AGATCC [GA]^GATC[CT] 712 AGATCC NdeII 608 GATC ^GATC 713 GATC NlaIV 433 GGTTCC GG[ACGT]^[ACGT]CC 571 GGCTCC Sau3AI 608 GATC ^GATC 713 GATC SfaNI 195 GATGC GCATC(5/9) 148 GCATC XhoII 607 AGATCC [GA]^GATC[CT] 712 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BbvI BsrI BstF5I BstXI Cac8I Fnu4HI FokI ItaI MboII MnlI MseI NlaIII Tru9I TseI Tsp509I TspRI