(Circular) (Six-base) MAPSORT of: pFN21AE1220.seq Check: 9350 from: 1 to: 5428 pFN21AE1220 5428 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2173 2173 Size: 5428 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2018 2261 3491 4875 5143 1632 Size: 386 243 1230 1384 268 1917 Fragments arranged by size: 1917 1384 1230 386 268 243 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 4327 5304 1203 Size: 3124 977 1327 Fragments arranged by size: 3124 1327 977 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5428 AlwNI CAG_nnn'CTG Cuts at: 3171 4743 3171 Size: 1572 3856 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2391 2737 2963 3115 5224 1493 Size: 898 346 226 152 2109 1697 Fragments arranged by size: 2109 1697 898 346 226 152 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2753 1870 Size: 73 810 4545 Fragments arranged by size: 4545 810 73 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2749 4204 618 Size: 325 217 627 962 1455 1842 Fragments arranged by size: 1842 1455 962 627 325 217 BanII G_rGCy'C Cuts at: 1391 2747 1391 Size: 1356 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 928 Size: 971 171 4286 Fragments arranged by size: 4286 971 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3619 3653 1903 Size: 34 50 34 1598 34 3678 Fragments arranged by size: 3678 1598 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2633 3320 4530 2633 Size: 687 1210 3531 Fragments arranged by size: 3531 1210 687 BclI T'GATC_A Cuts at: 1548 1548 Size: 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4039 136 Size: 107 122 71 631 605 2367 1525 Fragments arranged by size: 2367 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 2239 2275 3489 4645 1790 Size: 449 36 1214 1156 2573 Fragments arranged by size: 2573 1214 1156 449 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2533 2611 3159 Size: 416 53 83 399 24 1116 78 548 Cuts at: 3159 4117 442 Size: 958 1753 Fragments arranged by size: 1753 1116 958 548 416 399 83 78 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4088 1239 Size: 2849 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2515 3553 4418 4716 2515 Size: 1038 865 298 3227 Fragments arranged by size: 3227 1038 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4091 882 Size: 617 2592 2219 Fragments arranged by size: 2592 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2644 2682 3219 493 Size: 711 1440 38 537 2702 Fragments arranged by size: 2702 1440 711 537 38 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 3235 3617 4252 Size: 53 83 186 936 636 1066 382 635 Cuts at: 4252 275 Size: 1451 Fragments arranged by size: 1451 1066 936 636 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5203 Fragments arranged by size: 5203 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2569 1186 Size: 1383 4045 BseYI C'CCAG_C Cuts at: 2047 4631 5127 2047 Size: 2584 496 2348 Fragments arranged by size: 2584 2348 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4855 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2878 3639 4667 1540 Size: 275 186 877 761 1028 2301 Fragments arranged by size: 2301 1028 877 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3489 3574 4645 3489 Size: 85 1071 4272 Fragments arranged by size: 4272 1071 85 BsmI GAATG_Cn' Cuts at: 2939 3032 2939 Size: 93 5335 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2275 2747 3489 3574 4645 1391 Size: 399 449 36 472 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 449 399 85 36 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2173 2783 844 Size: 1329 610 3489 Fragments arranged by size: 3489 1329 610 BsrBI CCG'CTC Cuts at: 1394 1823 3315 1394 Size: 429 1492 3507 Fragments arranged by size: 3507 1492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2193 3923 4097 65 Size: 1183 945 1730 174 1396 Fragments arranged by size: 1730 1396 1183 945 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4072 4259 1700 Size: 110 2262 187 2869 Fragments arranged by size: 2869 2262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 2009 3488 4500 2009 Size: 1479 1012 2937 Fragments arranged by size: 2937 1479 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5428 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2352 2636 2164 Size: 188 284 4956 Fragments arranged by size: 4956 284 188 BstYI r'GATC_y Cuts at: 1972 2612 2717 2758 3125 3510 3527 4979 5065 Size: 640 105 41 367 385 17 1452 86 Cuts at: 5065 5077 5423 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 640 385 367 346 105 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4876 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2213 487 Size: 683 56 987 3702 Fragments arranged by size: 3702 987 683 56 BtsI GCAGTG_nn' Cuts at: 2237 2944 3738 3758 2237 Size: 707 794 20 3907 Fragments arranged by size: 3907 794 707 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2430 2732 2430 Size: 302 5126 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4435 809 Size: 507 3119 1802 Fragments arranged by size: 3119 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2875 3764 5393 Size: 54 1425 50 275 18 1045 889 1629 Cuts at: 5393 8 Size: 43 Fragments arranged by size: 1629 1425 1045 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2875 1537 Size: 275 1063 4090 Fragments arranged by size: 4090 1063 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 2255 3358 1353 Size: 684 218 1103 3423 Fragments arranged by size: 3423 1103 684 218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4015 4389 4535 387 Size: 1064 138 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2018 2261 3491 4088 4875 5143 1239 Size: 393 386 243 1230 597 787 268 1524 Fragments arranged by size: 1524 1230 787 597 393 386 268 243 EcoRI G'AATT_C Cuts at: 2737 3115 2737 Size: 378 5050 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 4575 1678 Size: 2897 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 2709 2741 4069 Size: 32 135 32 992 32 113 32 1328 Cuts at: 4069 4101 4143 4175 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1328 992 135 113 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2772 3018 669 Size: 988 281 834 246 3079 Fragments arranged by size: 3079 988 834 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5428 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 2350 3027 2350 Size: 677 4751 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 2248 4542 4726 1305 Size: 801 142 2294 184 2007 Fragments arranged by size: 2294 2007 801 184 142 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3606 Fragments arranged by size: 3606 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 3386 3745 3904 518 Size: 648 1032 1188 359 159 2042 Fragments arranged by size: 2042 1188 1032 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2553 2844 3521 4669 4914 1606 Size: 947 291 677 1148 245 2120 Fragments arranged by size: 2120 1148 947 677 291 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4876 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2103 2786 4331 1132 Size: 97 874 683 1545 2229 Fragments arranged by size: 2229 1545 874 683 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5428 PciI A'CATG_T Cuts at: 4327 4327 Size: 5428 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5428 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PmlI CAC'GTG Cuts at: 2682 2682 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PstI C_TGCA'G Cuts at: 830 2678 2780 830 Size: 1848 102 3478 Fragments arranged by size: 3478 1848 102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2674 2776 3911 4592 4783 826 Size: 219 1629 102 1135 681 191 1471 Fragments arranged by size: 1629 1471 1135 681 219 191 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2755 1872 Size: 883 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2494 3532 4433 4695 820 Size: 197 926 551 1038 901 262 1553 Fragments arranged by size: 1553 1038 926 901 551 262 197 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2103 2786 1132 Size: 971 683 3774 Fragments arranged by size: 3774 971 683 SspI AAT'ATT Cuts at: 5 52 2288 3352 5 Size: 47 2236 1064 2081 Fragments arranged by size: 2236 2081 1064 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5428 StyI C'CwwG_G Cuts at: 513 1065 2867 513 Size: 552 1802 3074 Fragments arranged by size: 3074 1802 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2515 3455 3472 3625 3810 4255 325 Size: 1255 935 940 17 153 185 445 1498 Fragments arranged by size: 1498 1255 940 935 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3674 5386 96 Size: 274 80 33 51 1443 1697 1712 138 Fragments arranged by size: 1712 1697 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5388 5420 5388 Size: 32 5396 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5428 XmaI C'CCGG_G Cuts at: 1870 2753 1870 Size: 883 4545 XmnI GAAnn'nnTTC Cuts at: 3557 3557 Size: 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI