(Linear) (Six-base) MAPSORT of: KIEE1220.seq  Check: 6502  from: 1  to: 723



KIEE1220.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     168     723 
   Size:        168     555
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      13     256     723 
   Size:         13     243     467

  Fragments arranged by size: 

                 467     243      13
 
ApoI r'AATT_y

Cuts at:      0     386     723 
   Size:        386     337
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      65     723 
   Size:         65     658
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     628     723 
   Size:        628      95
 
Bme1580I G_kGCm'C

Cuts at:      0     234     270     723 
   Size:        234      36     453

  Fragments arranged by size: 

                 453     234      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     528     606     723 
   Size:        528      78     117

  Fragments arranged by size: 

                 528     117      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     510     723 
   Size:        510     213
 
BsaAI yAC'GTr

Cuts at:      0     639     677     723 
   Size:        639      38      46

  Fragments arranged by size: 

                 639      46      38
 
BsaHI Gr'CG_yC

Cuts at:      0     164     723 
   Size:        164     559
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     564     723 
   Size:        564     159
 
BseYI C'CCAG_C

Cuts at:      0      42     723 
   Size:         42     681
 
Bsp1286I G_dGCh'C

Cuts at:      0     234     270     723 
   Size:        234      36     453

  Fragments arranged by size: 

                 453     234      36
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     168     723 
   Size:        168     555
 
BsrDI GCAATG_nn'

Cuts at:      0     188     723 
   Size:        188     535
 
BssSI C'ACGA_G

Cuts at:      0       4     723 
   Size:          4     719
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     159     347     631     723 
   Size:        159     188     284      92

  Fragments arranged by size: 

                 284     188     159      92
 
BstYI r'GATC_y

Cuts at:      0     607     712     723 
   Size:        607     105      11

  Fragments arranged by size: 

                 607     105      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     208     723 
   Size:        208     515
 
BtsI GCAGTG_nn'

Cuts at:      0     232     723 
   Size:        232     491
 
DraI TTT'AAA

Cuts at:      0     425     723 
   Size:        425     298
 
EarI CTCTTCn'nnn_

Cuts at:      0      32     250     723 
   Size:         32     218     473

  Fragments arranged by size: 

                 473     218      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      13     256     723 
   Size:         13     243     467

  Fragments arranged by size: 

                 467     243      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     559     591     704     723 
   Size:        559      32     113      19

  Fragments arranged by size: 

                 559     113      32      19
 
MfeI C'AATT_G

Cuts at:      0     345     723 
   Size:        345     378
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     243     723 
   Size:        101     142     480

  Fragments arranged by size: 

                 480     142     101
 
MslI CAynn'nnrTG

Cuts at:      0     193     723 
   Size:        193     530
 
MspA1I CmG'CkG

Cuts at:      0     548     723 
   Size:        548     175
 
NspI r_CATG'y

Cuts at:      0      98     723 
   Size:         98     625
 
PmlI CAC'GTG

Cuts at:      0     677     723 
   Size:        677      46
 
PstI C_TGCA'G

Cuts at:      0     673     723 
   Size:        673      50
 
SfcI C'TryA_G

Cuts at:      0     669     723 
   Size:        669      54
 
SmlI C'TyrA_G

Cuts at:      0     489     723 
   Size:        489     234
 
SphI G_CATG'C

Cuts at:      0      98     723 
   Size:         98     625
 
SspI AAT'ATT

Cuts at:      0     283     723 
   Size:        283     440
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     510     723 
   Size:        510     213
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     BbsI    BciVI Bme1580I     BmrI    BpuEI
    BsaAI    BsaHI    BseRI    BseYI Bsp1286I    BspMI    BsrDI    BssSI
    BstXI    BstYI    BtgZI     BtsI     DraI     EarI  Eco57MI    Hin4I
     MfeI     MmeI     MslI   MspA1I     NspI     PmlI     PstI     SfcI
     SmlI     SphI     SspI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI